Method for producing avian adenovirus inactivated vaccine through LMH clone line

An inactivated vaccine, avian adenovirus technology, applied in veterinary vaccines, biochemical equipment and methods, vaccines, etc., can solve the problems of low vaccine protection rate, low virus titer, poor cell viability, etc. High, reduce production costs, improve the effect of valence
CN106798918AInactive Publication Date: 2017-06-06广州博恒生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广州博恒生物科技有限公司
Publication Date
2017-06-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for producing avian adenovirus inactivated vaccine through an LMH clone line. The method comprises the following steps: firstly, preparation continuous cell line LMH cell; secondly, virus-seed reproduction; thirdly, virus collection, concentration and purification; fourthly, virus content determination; fifthly, inactivation and emulsification of virus, and forming emulsion inactivated vaccine. Compared with the traditional technical method for producing avian adenovirus through cell culture, the method has the advantages that high-quality inactivated vaccine with higher antigen titer through the optimization of the concentration of digestive LMH cell pancreatin-EDTA, the culture time of LMH cell and the inoculation concentration and harvest time of virus, injection immunization is performed at different doses, and all the vaccine protection rates reach 100 percent, so that the immune efficiency of the avian adenovirus type IV inactivated vaccine produced through the method is high, and the vaccine has complete immune protection effect on avian adenovirus type IV.
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Description

Technical field

[0001] The invention belongs to the technical field of veterinary biological products, and specifically relates to a method for producing an avian adenovirus inactivated vaccine by using an LMH cell line. Background technique

[0002] Avian group I adenovirus is a common infectious pathogen of poultry, and it has a worldwide distribution. It has been found that poultry of all ages are susceptible, but the pathogenicity is different in view of the breed and age of poultry. At present, it has been found that avian group I adenovirus is highly pathogenic to chickens. The main lesions are inclusion body hepatitis and pericardial effusion-hepatitis syndrome. The disease can directly cause more than 30% of chickens at 20-40 days old in chicken farms. In addition, it is also clinically often concurrently infected with infectious bronchitis virus, reovirus, H9 subtype avian influenza virus, etc., resulting in obvious respiratory diseases, arthritis, and severe egg product...

Claims

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