Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting microRNA associated with Salmonella enteritidis infection of chickens

A technology of Salmonella enteritidis and Salmonella, applied in the field of bioengineering

Inactive Publication Date: 2014-09-10
SHANDONG AGRICULTURAL UNIVERSITY
View PDF1 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far no studies have identified microRNAs associated with Salmonella enteritidis infection in chickens by Solexa sequencing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting microRNA associated with Salmonella enteritidis infection of chickens
  • Method for detecting microRNA associated with Salmonella enteritidis infection of chickens
  • Method for detecting microRNA associated with Salmonella enteritidis infection of chickens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0039] The white Legion laying hens were divided into the test group and the control group. The test group was inoculated with Salmonella Enteritidis, and the control group was inoculated with phosphate buffered saline (PBS). Seven days after inoculation, small intestinal tissue samples were collected, and total RNA was extracted with a kit.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting microRNA associated with Salmonella enteritidis infection of chickens. The microRNA associated with Salmonella enteritidis infection of chickens is identified by a Solexa high-throughput sequencing technology, the functions of microRNA-related target genes and signaling pathways on which the target genes act are further analyzed by bioinformatic related technologies, microRNA and the information thereof are integrated and microRNA associated with Salmonella enteritidis infection is screened. The seventh day of chickens infected with Salmonella enteritidis is selected as a sample collection point; a plurality of individuals in treated groups and control groups are sequenced in a mixed pool, the number of the mixed pools within each group is equal to or greater than 3; since microRNA associated with Salmonella enteritidis infections of chickens is analyzed and identified for the first time in a large scale, the method lays a foundation for deeply studying the mechanism of action of microRNA in Salmonella enteritidis infection and provides the theoretical basis and scientific basis for molecular disease-resistant genetic breeding of chickens.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to a method for detecting microRNA associated with Salmonella enteritidis gallinarum infection. Background technique [0002] Salmonella enteritidis (Salmonella enteritidis) is a Gram-negative bacterium and is one of the world-recognized food-borne pathogens. threaten. This kind of bacteria mainly causes human poisoning and even death through animal food sources, mainly including meat, eggs, milk and related products. Salmonella can invade cells and release toxins. The invasion process is to first form a needle-like protrusion on its surface to contact the target cell. Some special proteins reach the target cell through the needle-like protrusion and destroy the cell membrane structure, so that Salmonella cells can invade into the cell. Release of toxic proteins in target cells. [0003] MicroRNA is a kind of endogenous non-coding RNA with regulatory function found in eukaryo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6869C12Q1/689C12Q2600/178C12Q2535/122Y02A50/30
Inventor 李显耀吴桂贤刘丽英齐玉凯王莎莎
Owner SHANDONG AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com