Nanometer carboxylated polystyrene microsphere with spacer arm and preparation method thereof

A technology of polystyrene microspheres and spacer arms, which is applied in the field of nano-scale carboxylated polystyrene microspheres and its preparation, can solve the problems of steric hindrance, influence the coupling rate and biological activity of biological macromolecules, etc. Steric hindrance, simple and economical equipment, improved coupling rate and utilization effect

Inactive Publication Date: 2012-06-13
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When coupling biomacromolecules on the surface of microspheres, it will inevitably cause certain steric hindrance, which will affect the coupling rate and biological acti

Method used

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  • Nanometer carboxylated polystyrene microsphere with spacer arm and preparation method thereof
  • Nanometer carboxylated polystyrene microsphere with spacer arm and preparation method thereof
  • Nanometer carboxylated polystyrene microsphere with spacer arm and preparation method thereof

Examples

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Embodiment 1

[0027]Wash styrene four times with 5% NaOH solution, then wash styrene with distilled water until neutral, separate the water layer, add anhydrous calcium chloride and dry overnight. The dried styrene was distilled under reduced pressure and stored in a refrigerator at 4°C. Preparation concentration is the potassium persulfate (KPS) aqueous solution of 0.05%, pipettes the potassium persulfate solution of 40ml and joins in the Erlenmeyer flask of 250ml, adds 0.0024g (0.006%) sodium dodecylsulfonate (SDS), waits for SDS After completely dissolving, add 4ml of mixed monomers of styrene and acrylic acid (the mass ratio of mixed monomers to water phase is 1:9), wherein 3.6ml of styrene and 0.4ml of acrylic acid. Nitrogen was passed through for 10 minutes to remove oxygen, and then emulsified on a shaker for 30 minutes. The flask was placed in a constant temperature water bath shaker at 60° C., and reacted for 10 h at a speed of 200 rpm to prepare carboxylated polystyrene microsphe...

Embodiment 2

[0030] Wash styrene four times with 5% NaOH solution, then wash styrene with distilled water until neutral, separate the water layer, add anhydrous calcium chloride and dry overnight. The dried styrene was distilled under reduced pressure and stored in a refrigerator at 4°C. Preparation concentration is the potassium persulfate (KPS) aqueous solution of 0.1%, pipettes the potassium persulfate solution of 40ml and joins in the Erlenmeyer flask of 250ml, adds 0.016g (0.04%) sodium dodecylsulfonate (SDS), waits for SDS After completely dissolving, add 4ml of mixed monomers of styrene and acrylic acid (the mass ratio of mixed monomers and water phase is 1:9), wherein 3.8ml of styrene and 0.2ml of acrylic acid. Nitrogen was passed through for 10 minutes to remove oxygen, and then emulsified on a shaker for 30 minutes. The flask was placed in a constant temperature water bath shaker at 70°C, and reacted for 16 hours at a speed of 200 rpm to prepare carboxylated polystyrene microsph...

Embodiment 3

[0033] Wash styrene four times with 5% NaOH solution, then wash styrene with distilled water until neutral, separate the water layer, add anhydrous calcium chloride and dry overnight. The dried styrene was distilled under reduced pressure and stored in a refrigerator at 4°C. Preparation concentration is the potassium persulfate (KPS) aqueous solution of 0.3%, pipettes the potassium persulfate solution of 40ml and joins in the Erlenmeyer flask of 250ml, adds 0.08g (0.2%) sodium dodecylsulfonate (SDS), waits for SDS After completely dissolving, add 4ml of mixed monomers of styrene and acrylic acid in total (the mass ratio of mixed monomers and water phase is 1:9), wherein styrene is 3.98ml, and acrylic acid is 0.02ml. Nitrogen was passed through for 10 minutes to remove oxygen, and then emulsified on a shaker for 30 minutes. The flask was placed in a constant temperature water bath shaker at 80°C, and reacted at a speed of 200 rpm for 24 hours to prepare carboxylated polystyren...

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Abstract

The invention relates to a nanometer carboxylated polystyrene microsphere with a spacer arm and a preparation method thereof. A nanometer carboxylated polystyrene microsphere A is prepared by taking styrene and acrylic acid as a mixed monomer, taking water as a reaction medium, taking potassium persulfate as an initiator and taking sodium dodecyl sulfate as an emulsifier with an emulsion polymerization method. In the presence of EDC (Ethylidene Dichloride) serving as a coupling agent and Sulfo NHS, 4-aminobutyric acid, 6-amidocaproic acid or 8-aminocaprylic acid is connected to the surface of the microsphere, so that the nanometer carboxylated polystyrene microsphere with the spacer arm is prepared. The spacer arm is introduced into the surface of the nanometer microsphere, so that the steric hindrance of a coupling macromolecule is lowered, the activity and utilization ratio of the macromolecule are increased, and a nonspecific interaction between the macromolecule and the polystyrene microsphere is reduced. Meanwhile, the grain diameter of the microsphere can be adjusted by adjusting the using amount of the emulsifier, the length of the space arm and surface density, so that the nanometer microsphere has a wide application prospect in the fields of immune diagnosis, solid phase extraction and the like when serving as a carrier as well as a template.

Description

technical field [0001] The invention belongs to the field of preparation of new nanometer materials, and mainly relates to a nanoscale carboxylated polystyrene microsphere with spacer arms and a preparation method. Background technique [0002] Polymer microspheres have the characteristics of good sphericity, small size, large specific surface area, and the surface is easy to be endowed with various functional groups by means of copolymerization and modification. application. Especially in the field of biological sciences, it is of great significance to the research of clinical diagnosis, testing, immunology, cytology, etc. , 2000, 18(4): 31) Since the 1970s, many researchers have developed a variety of effective microsphere preparation methods such as emulsion polymerization, microemulsion polymerization, dispersion polymerization and suspension polymerization. Emulsion polymerization is one of the main methods for the design and synthesis of microspheres, which can prepa...

Claims

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Application Information

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IPC IPC(8): C08F212/08C08F220/06C08F2/26C08F8/34C08F8/32C08J7/12
Inventor 王冬梅徐亮赵海峰
Owner TIANJIN MEDICAL UNIV
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