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Preparation method of rare-earth fluorescent silica nano particle

A technology of silica and nanoparticles, applied in chemical instruments and methods, nanotechnology, nano-optics, etc., can solve the problems of wide application of unfavorable markers, narrow emission spectrum band, time-consuming and labor-intensive, etc., to achieve easy biological functionalization, The preparation process is simple and the controllability is good

Active Publication Date: 2012-06-20
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

④ Emission spectral band is narrow
However, this cyclic bonding method is cumbersome, time-consuming and labor-intensive, which is not conducive to the wide application of this marker

Method used

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  • Preparation method of rare-earth fluorescent silica nano particle
  • Preparation method of rare-earth fluorescent silica nano particle
  • Preparation method of rare-earth fluorescent silica nano particle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Preparation of BHHCT-Eu silica nanoparticles

[0031] This example describes the preparation of blank silica nanoparticles in a water-in-oil microemulsion system, and then directly adding the pre-reacted rare earth complex precursor APTMS-BHHCT to the microemulsion system to modify the surface of the particles, and then adding Eu 3+ The process of preparing rare earth fluorescent silica nanoparticles by chelating rare earth ions with rare earth ion complex BHHCT.

[0032] (1) Preparation of blank silica nanoparticles in water-in-oil microemulsion system: Take a conical flask with a magnet, add 6mL cyclohexane, 2mL n-hexanol, 2mL Triton X-100, 0.6 ml ultrapure water to form a water-in-oil microemulsion system. After the solution was uniformly mixed, 60 μL of ammonia water (25%-28%) and 100 μL of TEOS were added in sequence, and reacted at room temperature for 24 hours. Under the catalysis of ammonia water, TEOS polymerized in the water core to form blank ...

Embodiment 2

[0036] Example 2: Application of Rare Earth Fluorescent Silica Nanoparticles in Rapid Immunochromatographic Detection

[0037] This example describes the implementation process of a rapid immunochromatographic detection system using rare earth fluorescent silica nanoparticles as markers and hepatitis B virus surface antigen (HBsAg) as a detection model.

[0038] (1) Preparation of rare earth fluorescent silica nanoparticles: same as in Example 1.

[0039] (2) Antibody labeling: Take 0.3mg anti-hepatitis B surface antigen monoclonal antibody and dialyze against 0.05M sodium acetate buffer (pH5.2) for 6h, then add NaIO to the antibody 4 To a final concentration of 0.01M, react at room temperature for 20 minutes to oxidize the hydroxyl groups of antibody sugar chain molecules into aldehyde groups. Then add glycerol to its final concentration of 30mM, mix and shake for 10min to stop the oxidation reaction. The antibody was again dialyzed with 0.05M sodium acetate buffer (pH5.2) ...

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Abstract

Provided is a preparation method of rare-earth fluorescent silica nano particle, relating to nano material particles. The preparation method comprises the following steps:: mixing water, oil, surfactant and co-surfactant to spontaneously form anisotropic transparent disperse system with stable thermodynamics, so as to obtain silica nano particle with hydroxyl at the surface thereof; reacting silane reagent with functional group with rare earth ionic complex to obtain rare earth ionic complex precursor; mixing the prepared rare earth ionic complex precursor with tetraethoxy silane, and then adding the mixture into the obtained silica nano particle with hydroxyl at the surface thereof, wherein the rare earth ionic complex is bonded on the surface of the silica nano particle; and adding rare earth ions into a water-in-oil micro-emulsion system to form fluorescent rare earth complex by chelating with the earth ionic complex fixed on the surface of the silica nano particle, sealing the reaction system by acetone, and rinsing to obtain the product rare-earth fluorescent silica nano particles. The operation is simple, the implementation is easy and the fluorescent intensity is higher.

Description

technical field [0001] The invention relates to a nano particle material, in particular to a preparation method of high-strength rare earth fluorescent silica nano particles. Background technique [0002] Immunoassays are increasingly used in the detection of biomarkers, bacteria, and viruses. The nanomarkers used in immunoassay largely determine the sensitivity and specificity of the assay. Various types of nanoparticles that have been developed, including colloidal gold, carbon black, up-conversion fluorescent nanoparticles, quantum dots, liposomes, etc., have shown excellent immunoassay results. However, these nanolabels still have their own shortcomings. For colloidal gold, carbon black markers, etc., they only rely on particle aggregation to generate optical signals, thus showing low sensitivity in immunochromatography. Although the later development of fluorescent nanolabels has greatly improved the sensitivity of immunoassays, they also have some drawbacks, such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N33/577B82Y20/00
Inventor 李庆阁王国磊许晔
Owner XIAMEN UNIV
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