Preparation method and product of germinated wheat rich in gamma-aminobutyric acid

A technology of aminobutyric acid and wheat kernels, which is applied in the field of preparation of germinated wheat kernels rich in γ-aminobutyric acid, can solve the problems of poor effect and unstable yield, and achieve the goal of wide sources, simple operation, and promotion of enzyme activity Effect

Inactive Publication Date: 2013-11-20
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the invention of the present invention is: for the method for preparing gamma-aminobutyric acid by the existing bioenrichment method, mainly utilize tealeaves, rice product to enrich GABA, there are disadvantages such...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Select whole and plump wheat grains with no defects in the wheat germ as raw materials, wash them with water three times to remove the dust on the surface of the wheat grains, and then soak them in alcohol for 10 minutes with an alcohol concentration of 75%. After soaking, wash with sterile water 3 times to remove alcohol, and finally dry the moisture on the surface of the wheat grains with filter paper, so that the wheat grains can be pretreated for later use. This step can ensure that the pretreated grains are free from mold contamination in the subsequent process.

[0034] Transfer the pretreated wheat kernels to a petri dish, then add compound culture solution to the petri dish, submerge the pretreated wheat kernels in the compound culture solution, let the pretreated wheat kernels fully absorb the compound culture solution for 1 hour, and then transfer the petri dish to the incubator The germination culture was carried out in a dark place, the temperature in the in...

Embodiment 2

[0038] Select whole and plump wheat grains with no defects in the wheat germ as raw materials, wash them with water 5 times to remove the dust on the surface of the wheat grains, and then soak them in alcohol for 5 minutes with an alcohol concentration of 90%. After soaking, wash it twice with sterile water to remove the alcohol, and finally use filter paper to dry the moisture on the surface of the wheat grains, so that the wheat grains can be pretreated and set aside. This step can ensure that the pretreated grains are free from mold contamination in the subsequent process.

[0039] Transfer the pretreated wheat kernels to the petri dish, then add the compound culture solution to the petri dish, submerge the pretreated wheat kernels in the compound culture solution, let the pretreated wheat kernels fully absorb the compound culture solution for 2 hours, and then transfer the petri dish to the incubator The germination culture was carried out in a dark place, the temperature ...

Embodiment 3

[0043] Select whole and plump wheat grains with no defects in wheat germ as raw materials, wash them with water twice to remove the dust on the surface of the wheat grains, and then soak them in alcohol for 18 minutes with an alcohol concentration of 75%. After soaking, wash 5 times with sterile water to remove alcohol, and finally dry the moisture on the surface of the wheat grains with filter paper, so that the wheat grains can be pretreated and set aside.

[0044] Transfer the pretreated wheat kernels to a petri dish, then add compound culture solution to the petri dish, submerge the pretreated wheat kernels in the compound culture solution, let the pretreated wheat kernels fully absorb the compound culture solution for 1.5 hours, and then transfer the petri dish to the culture dish Germination was carried out in an incubator protected from light. The temperature in the incubator was 37° C., and the incubation time was 7.5 hours to obtain temperature-controlled cultured whea...

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Abstract

The invention discloses a preparation method and product of germinated wheat rich in gamma-aminobutyric acid, and the method is used for solving the problems of bad existing effect and unstable yield since GABA is enriched by utilizing tea and rice products according to an existing method for preparing gamma-aminobutyric acid based on a biological concentration method. The method comprises the following steps of: raw material selection, pretreatment, imbibition, temperature control training and germination termination. Wheat seeds are dramatically taken as raw materials, the improvement of GAD enzyme activity in wheat seeds is effectively promoted by controlling germination conditions, a cultivated substrate and the pH value, and meanwhile the GAD activity is ensured by controlling the pH value, the activity of GABA aminopherase is inhibited, and the content of gamma-aminobutyric acid in germinated wheat is greatly improved, so that the nutritive value of wheat is greatly improved. Experimental results show that the content of GABA in the prepared germinated wheat is 31.08 mg per 100 g at most, and achieves domestic and international leading level of similar methods.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a method for preparing germinated wheat grains rich in gamma-aminobutyric acid and a product thereof. The grains are processed to prepare wheat grains rich in gamma-aminobutyric acid. Background technique [0002] The chemical name of γ-aminobutyric acid is: 4-aminobutyric acid, and the molecular formula is: NH 2 CH 2 CH 2 CH 2 COOH, abbreviated as: GABA, whose amino group is at the γ-C position, is a natural amino acid that is not composed of proteins. [0003] γ-aminobutyric acid is a naturally occurring functional amino acid and an important inhibitory neurotransmitter. It exists in a small amount in the brain and bone marrow of mammals; in addition, it is contained in vegetables and fruits A certain amount of gamma-aminobutyric acid, but the content is very small. As one of the important inhibitory neurotransmitters in the animal brain, γ-aminobutyric acid plays a very imp...

Claims

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Application Information

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IPC IPC(8): A23L1/185A23L1/305A23L33/175
Inventor 张志清徐杰刘虹白琦申光辉韩新锋杨勇秦文
Owner SICHUAN AGRI UNIV
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