L-2-reanal biological preparation method

Abstract

The invention relates to an L-2-reanal biological preparation method, which includes: utilizing L-threonine as raw materials, stirring the L-threonine in water at the temperature of 15-50 DEG C under the catalytic action of L-threonine deaminase and whole cells with a leucine dehydogenase and coenzyme regenerating function, and obtaining L-2-reanal after reaction. In the L-2-reanal biological preparation method, water is used for substituting for buffer salt solution to form a water-phase reaction system, cost of raw materials is reduced, threonine deaminase and whole cells used for catalyzing can be produced in batch by fermentation of microorganisms, are low in cost and wide in sources. Biological enzymatic catalysis reaction is water-phase reaction, transformation conditions of the enzymic method are mild, raw materials are transformed thoroughly, post-treatment is simple, products are separated by isoelectric point crystallization technology and film evaporation separation technology jointly, and the L-2-reanal biological preparation method is capable of recovering water and ammonium formate is low in cost, free of discharge of waste water and waste residues, environment-friendly in process and applicable to industrialized production of the L-2-reanal.

Description

technical field [0001] The invention relates to a biological preparation method of L-2-aminobutyric acid. Background technique [0002] L-2-aminobutyric acid is a natural amino acid that inhibits the transmission of human nerve information. As an important chemical raw material and pharmaceutical intermediate, it has been widely used in drug synthesis. L-2-aminobutyric acid is the main raw material for the synthesis of the new antiepileptic drug levetiracetam, and it is also the key chiral precursor for the synthesis of the antibacterial and anti-tuberculosis drug ethambutol hydrochloride; A chiral intermediate of a chiral drug. [0003] Limited by technology and cost, the output of L-2-aminobutyric acid in my country cannot meet the demand of domestic and foreign trade export. There are chemical and biological methods for the preparation of L-2-aminobutyric acid. The traditional chemical methods, whether organic synthesis or chemical resolution, lose their competitivenes...

AI Technical Summary

Problems solved by technology

However, in the method of the invention, the three enzymes need to be cloned and expressed, then the cells are crushed and freeze-dried to obtain a freeze-dried powder enzyme preparation, which increases the cost of the catalyst; the reaction system needs to use phosphate as a buffer system, which increases the cost of raw materials . The post-reaction treatment of this process is only a preliminary treatment. According to this process, impurities such as inorganic salts and ammonium formate in the product are difficult to meet the requirements of raw materials