The invention discloses a micro-molecular nucleotide adaptor medicament for hepatitis C virus and application thereof. The DNA adaptor sequence is the nucleotide sequence represented by SEQ IN No. 1-29. The method comprises the following steps: firstly, building a random single-chain DNA library; secondly, synthesizing a double-chain DNA library; thirdly, SELEX selecting; fourthly, PCR amplifying; fifthly, cloning and detecting sequence; and sixthly, testing DNA adaptor efficiency by level tests on cells in vitro. The micro-molecular nucleotide adaptor is applied to preparing medicaments in treating hepatitis C virus and preventing hepatitis C virus infecting human cells. The DNA adaptor can be directly used as agonist and diagnostic reagent of hepatitis C virus, can be used for preventing, detecting and treating hepatitis C virus, and overcomes the defects that combined treatment with alpha interferon and ribavirin has strong side effect when alpha interferon or antivirus medicaments such as alpha interferon, ribavirin and the like which are combined are used for treating hepatitis C virus in clinic, and that hepatitis C virus has no vaccine to prevent currently.