37 results about "Rabbit brain" patented technology

The invention discloses a hepaplastin test determination kit and a preparation method thereof. Through combination of a buffer solution system a containing multiple effective components, plasma without blood coagulation factors VII, II, X and IX, a buffer system b, rabbit brain powder and tissue factor ester, the hepaplastin test determination kit improves reagent stability. A hepaplastin test determination reagent with good accuracy, good repeatability and good effects is further obtained.

The present invention provides one kind of brain extract extracted from brain tissue of mammal, including pig, dog, ox, sheep, deer, horse and rabbit. The brain extract contains polypeptide, free lysine, free glutamic acid and total sugar in the weight ratio of 1-200 to 0.1-100 to 0.1-80 to 0.1-12, and has molecular weight of 5,000-10,000. The brain extract contains active matters capable of regulating and improving cerebral metabolism and may be used in treating cerebral vascular diseases and corresponding cerebral metabolic disturbance sequela.

The invention relates to an egg laying poultry feed additive for supplying and adding a dietary factor. The constituents and the weight percentages thereof are as follows: soy protein bioactive peptides powder 25-30%, allium mongolicum powder 15-20%, sunflower seed powder 2%-5%, linseed 3%-5%, hemp seed powder 3%-5%, rabbit brain powder 5%-10%, perillaseed powder 5%-10%, black sesame seed powder 5%-10%, parslane herb powder 5%-10%, brown sugar 5%-10%, chicken's gizzard-membrane powder 2%-5%, dried orange peel powder 3%-5%, and cucumber seed powder 2%-5%. The egg laying poultry are fed with the feed additive and basal feed by the ratio of 1:10-1:20. Through the transformation of the feed additive in the egg laying poultry bodies, poultry eggs with low cholesterol and high HDL content (about 15 mg) are obtained. The poultry eggs eaten by people can increase the HDL level in human body by 80% and above, obviously relive the symptom of arteriosclerosis, and radically cure cardio-cerebrovascular disease after people eat the eggs for a long period. The feed additive has the advantages of high effective constituents and rich functional constituents. The materials in the feed additive are natural plants which grow widely, are easy to grow, get and free of toxins, can be eaten by people, fed to the poultry and used as medicine, and is suitable for the development and application of the poultry eggs with high nutritional value and health protection function.

The invention discloses a preparing method for pig thrombin freeze-dried powder. The preparing method comprises the following steps that anticoagulant pig plasma and gel are mixed and stirred for adsorption and pass through a column for elution, and a prothrombin solution is obtained; normal saline is added to rabbit brain powder, then the prothrombin solution and CaCl2 are added for zymogen activation, a crude thrombin enzyme solution is obtained, subjected to ultrafiltration for desalination and concentrated, viral inactivation is carried out, an obtained crude enzyme solution passes through DEAE-Sepharose Fast Flow chromatographic column, elution is carried out, target peaks are collected, and pig thrombin is obtained; the pig thrombin is added with mannitol or dextran 40, filtering, sterilizing, subpackaging, freeze drying and vacuum tamponing are carried out, an aluminum plastic combined cover is rolled, and a pig thrombin freeze-dried product is obtained; the freeze-dried product is subjected to dry heat treatment at 100 DEG C for secondary virus inactivation, and the pig thrombin freeze-dried powder is obtained after packaging. The specific activity of pig thrombin in the product is not lower than 130 U/mg, the whole process is simple in step and easy to implement, the product safety is improved, and the preparing method is suitable for industrial production.

The invention relates to a thromboelastography heparin quantitative detection kit, which comprises a buffer agent, a blood coagulation factor Xa, rabbit brain congealed fat, a coagulation factor activator, a supporting agent, and a biological preservative. The preparation method comprises the following steps: preparing a buffer solution, a blood coagulation factor Xa stock solution, a blood coagulation factor activator stock solution, a biological preservative solution, and a rabbit brain congealed fat stock solution; taking the support agent and adding the buffer solution to prepare a supportsolution; adding the blood coagulation factor Xa, the blood coagulation factor activator, and the rabbit brain congealed fat stock solution to the supporting solution according to the requirements ofkit specification, adding the biological preservative solution after uniformly mixing, then adding the buffer solution to the specified amount, subpackaging and lyophilizing after uniformly mixing. The thromboelastography heparin quantitative detection kit provided by the invention can quantitatively detect the heparin in a sample by a thromboelastography, has good correlation in a linear range,meets the heparin detection limit standard and has a coefficient of variation of not more than 10%, and the sample adopts whole blood without extra treatment; the integrity of protein in a clotting cascade is also not required, and the inspection operating process is simplified, thereby facilitating doctors and patients.

The invention discloses a prothrombin time assay kit and a preparation method thereof. The prothrombin time assay kit, which combines a buffer liquid system which contains a plurality of active ingredients with rabbit brain powder and a tissue factor ester, solves the problem that a single-ingredient reagent is incontrollable in sensitivity; and subsequently, a prothrombin time assay reagent, which is high in accuracy, strong in repeatability and good in stability, and is applicable to various semiautomatic/full-automatic coagulometers, is obtained.

The invention relates to the field of medical detection, in particular to an extraction buffer solution, a rabbit brain extraction solution, a PT detection reagent and a PT detection kit. The extraction buffer solution comprises citrate or phosphate, anhydrous sodium acetate and water. With adding of the electrolyte, the repulsive force of charges between phospholipids is increased, so that the PTreagent is not easy to precipitate. The stability is enhanced; and the repeatability during testing is improved.

The invention relates to a fibrinogen detection reagent/freeze-dried detection reagent for completely inhibiting the function of platelets and a preparation method of the fibrinogen detection reagent/freeze-dried detection reagent. The detection reagent comprises a buffer solution, a supporting agent, rabbit brain congealed fat, a tissue factor, a platelet inhibitor, a platelet inhibiting enhancer, an organic solvent and a biological preservative; the freeze-dried detection reagent is a quantitative bottled freeze-dried product of the above detection reagent. According to the preparation method of the detection reagent, a buffer solution, a supporting liquid, a rabbit brain congealed fat liquid, a tissue factor liquid, a platelet inhibiting liquid, a platelet inhibiting enhancing liquid and the like are prepared; the liquids are mixed, and a biological preservative is added into an obtained mixture; and the supporting liquid is added to a newly obtained mixture in a full-dose manner. According to the preparation method of the freeze-dried detection reagent, the detection reagent is quantitatively bottled and frozen to be dried. According to the detection reagent/freeze-dried detection reagent provided by the invention, a thrombelastogram detection method is adopted, the function of platelets can be completely inhibited with no additional fibrinogen activators required; the false positive result of fibrinogens can be avoided; the accuracy and stability of detection can be ensured; a process can be simplified; and reagents can be saved.

The invention provides an ellagic acid reagent and a preparation method thereof as well as an activated partial thromboplastin time (APTT) determination reagent and an APTT kit and relates to the field of clinical diagnosis reagents. The preparation method comprises the following steps: adding ellagic acid into calcium-free purified water and rapidly stirring to uniformly disperse the ellagic acid; adding an alkaline buffering solution or inorganic alkali; rapidly adding an acidic buffering solution or organic acid and inorganic acid within 10min after the ellagic acid is added; adding metal ions; adding amino acid and a derivative thereof to obtain a buffering solution; furthermore, adding the calcium-free purified water into rabbit brain phospholipid and uniformly mixing and emulsifyingto obtain emulsified phospholipid; adding the emulsified phospholipid into the buffering solution and rapidly stirring and uniformly mixing to obtain the ellagic acid reagent. The technology is simpleand rapid and has detailed descriptions. The activated partial thromboplastin time determination reagent comprises the ellagic acid reagent and a calcium chloride reagent and has good stability. TheAPTT kit containing the activated partial thromboplastin time determination reagent can be used for directly replacing an imported high-stability APTT kit.

The invention discloses an in-vitro diagnostic kit for measuring prothrombin time. The in-vitro diagnostic kit comprises a prothrombin time measuring reagent and a plastic bottle for containing the prothrombin time measuring reagent, wherein the prothrombin time determination reagent is composed of a buffer solution system and thromboplastin, and the thromboplastin is obtained by taking rabbit brain powder as a raw material through extraction; the buffer solution system is prepared from the following raw materials: a buffer agent, a calcium ion reagent, a surfactant, amino acid, trehalose andprocline 300. In the kit disclosed in the present invention, the buffer solution system is creatively prepared by compounding the buffer agent, the calcium ion reagent, the surfactant, the amino acid,the trehalose and the proclin 300; through mutual cooperation and synergy with thromboplastin, the stability of the prothrombin time determination reagent can be improved on the basis of ensuring theaccuracy and sensitivity of the prothrombin time determination reagent.

The invention discloses a processing method for industrially producing rabbit brain powder. The method is characterized by comprising the following processes of: taking fresh rabbit brain, soaking the rabbit brain into cold acetone, fishing out the rabbit brain, removing blood vessels and meninges, adding the treated rabbit brain into the cold acetone, mixing the rabbit brain and the cold acetoneuniformly by using a homogenizer, performing centrifugation to remove the acetone solvent, and drying the rabbit brain powder. The method has the advantages that: because the traditional mortar for grinding is substituted by using the homogenizer, the activity of tissue factors in the rabbit brain is kept; the taken fresh rabbit brain is directly soaked into the cold acetone to substitute the traditional brine washing, so that the velocity of removing the blood vessels and the meninges in the rabbit brain is improved and the activity of the tissue factors is improved; the velocity of gas jet impact drying for rabbit brain powder protection with gas is improved by 2 to 3 times compared with the velocity of the traditional vacuum drying, and the rabbit brain powder is thinner, higher in activity and purer in color; and the whole method is high in production efficiency, and can realize industrialized large-scale production.

Disclosed is a cranial nerves rehabilitation medicine and its preparation method, wherein the medicament is prepared from the following raw materials (by weight portion), rabbit brain 100-200, lichen 100-200, white fungus 50-70, buthus martensi kirsch 100-200, silkworm 80-150, earthworm 80-150, rhizoma Gastrodiae 80-150, Chinese angelica root 50-100, rhizome of Sichuan lovage 100-200, Sichuan aconite root 50-100, lubei 80-150, tabasheer 100-200, southern star 100-200, draconic dentes 80-150, amber 80-150, polygala root 80-150, calamus 50-80, Chinese data seeds 100-200.

The invention discloses a feed additive for promoting growth. The additive is prepared from 11 parts of soybean protein active peptide powder, 10 parts of allium mongolicum regel powder, 5 parts of sunflower seed powder, 10 parts of flaxseed powder, 8 parts of rabbit brain powder, 9 parts of black sesame powder, 12 parts of brown sugar, 3 parts of endothelium corneum gigeriae galli powder, 11 parts of potassium iodide, 3 parts of sodium selenite, 2 parts of swallow bone powder and 3 parts of pomegranate peel. Thus, rich nutrients are provided, the additive can be easily absorbed by poultry by being added into feed, the feed absorption rate is increased, and palatability of the feed is improved.

The present invention provides a preparation method of thrombin. The preparation method comprises that a prothrombin activator is added to a prothrombin-containing solution to activate prothrombin soas to obtain a thrombin-containing solution, and the prothrombin activator is selected from a recombinant lipidated tissue factor or a recombinant lipidated tissue factor and calcium ions. The methodfurther includes preparing the prothrombin-containing solution before activating prothrombin. The method further comprises the step of purifying and further treating the thrombin-containing solution to obtain a thrombin finished product. According to the preparation method disclosed by the invention, the recombinant lipidated tissue factor is used as an activator, so that compared with rabbit brain powder, the safety of a thrombin product can be remarkably improved, and the production cost is reduced; compared with soybean phospholipid and egg yolk phospholipid, the thrombin specific activityis obviously improved, and the method is very suitable for modern large-scale industrial production.

The invention relates to a traditional Chinese medicine for tonifying kidney, strengthening heart and spleen, and enhancing function of human body, and a preparation method thereof. The preparation method comprises the following steps: adding water into ten gouts of traditional Chinese medicine comprising prepared rhizome of rehmannia, angelica, sauted white paeony root, asparagus, dwarf lilyturf turber, sauted Atractylis ovata, desertliving cistanche, tuckahoe with pine, sauted spina date seed and honey-soaked milkwort, then decocting the mixture thrice, mixing the thrice decoction, condensing filtered decoction into extract, adding red ginseng, dog kidney, rabbit brain, amber, natural indigo, sharpleaf galangal fruit and grassleaf sweelflag rhizome into the extract, crushing and sieving the components respectively, then evenly mixing the fine powder, adding proper amount of starch into the fine powder to prepare granules, and filling the granules into hollow capsules to obtain the traditional Chinese medicine capsule. The traditional Chinese medicine not only has good curative effect on kidney essence deficiency and heart-spleen deficiency, and has the efficacies of replenishing qi to invigorate the spleen, enriching blood to nourish the heart, nourishing the kidney and replenishing essence, but also has the advantages of short treatment course by oral administration, quick effect, little toxic and side effects, and convenience for carrying, and simultaneously has the advantages of rich raw material sources, simple preparation method and low cost.

The invention provides a polypeptide-factor cosmetic and a preparation method thereof. The polypeptide-factor cosmetic is prepared from the following raw materials in parts by weight: 0.2 to 0.6 part of carbomer 974, 0.5 to 3.5 parts of honey, 0.5 to 1.5 parts of a ginseng extract, 0.5 to 3.5 parts of a lecithin extract, 4-8 parts of a plant extract, 6-10 parts of a rabbit brain extract, 4 to 8 parts of xanthan gum, 0.5 to 1.5 parts of peregal, 0.5 to 3.5 parts of glycerol, 0.8 to 1.2 parts of octadecanol, 0.5 to 1.5 parts of hexadecanol, 0.5 to 1.5 parts of glyceryl stearate, 0.5 to 3.5 parts of ethylparaben, 2-6 parts of hyaluronic acid and 6-10 parts of sodium hydroxide. The polypeptide-factor cosmetic can be used for effectively decomposing and fading black spots and chromatosis, improving skin color, diminishing inflammation, removing acnes, resisting aging and preventing wrinkles; and the polypeptide-factor cosmetic can be used for resisting oxidation and protecting skin from being damaged by ultraviolet rays, is safe and non-toxic, is easily available in raw materials and is suitable for being industrially produced.