A prothrombin time assay kit and preparation method thereof

A prothrombin time and kit technology, applied in biochemical equipment and methods, biological testing, and microbial determination/inspection, etc., can solve problems such as uncontrolled sensitivity of single-component reagents, and achieve high accuracy and stability. Good and repeatable effect

Active Publication Date: 2019-11-22
WUHAN KING DIAGNOSTIC TECH CO LTD
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the deficiencies in the above-mentioned existing background technology, the present invention provides a prothrombin time assay kit and a preparation method thereof. Combining with tissue factor esters solves the problem of uncontrolled sensitivity of single-component reagents, and further obtains prothrombin time determination reagents with better overall performance in terms of accuracy, repeatability and stability

Method used

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  • A prothrombin time assay kit and preparation method thereof
  • A prothrombin time assay kit and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Prothrombin time assay kit, including prothrombin time assay reagent, prothrombin time assay reagent consists of buffer system and thromboplastin dissolved in the buffer system, thromboplastin includes rabbit brain powder and tissue For factor esterification, the consumption of rabbit brain powder in the buffer system accounts for 7wt% of the buffer weight, and the consumption of tissue factor esterification in the buffer system accounts for 0.25wt% of the buffer weight; in the buffer system, the content of each component is : 0.32wt% TAPSO, 1.36wt% glycine, 0.92wt% polyethylene glycol 6000, 0.47wt% sodium sulfate, 0.03wt% sodium azide, 0.16wt% Brij-35, 14mM Calcium chloride, the rest is water, and the pH value of the buffer solution system is 5.0.

[0024]The method for preparing the prothrombin time assay kit comprises the following steps: (1) preparing a buffer system according to different proportions, warming the bath, weighing rabbit brain powder and adding the bu...

Embodiment 2

[0027] Prothrombin time assay kit, including prothrombin time assay reagent, prothrombin time assay reagent consists of buffer system and thromboplastin dissolved in the buffer system, thromboplastin includes rabbit brain powder and tissue For factor esterification, the consumption of rabbit brain powder in the buffer system accounts for 8wt% of the buffer weight, and the consumption of tissue factor esterification in the buffer system accounts for 0.1wt% of the buffer weight; the content of each component in the buffer system is : 0.48wt% TAPSO, 1.8wt% glycine, 1.0wt% polyethylene glycol 6000, 0.4wt% sodium sulfate, 0.2wt% sodium azide, 0.01wt% Brij-35, 20mM chlorine Calcium chloride, the rest is water, and the pH value of the buffer solution system is 6.3.

[0028] The preparation method of the prothrombin time assay kit is the same as in Example 1.

[0029] Apply the test kit obtained in this embodiment to measure the prothrombin time of the plasma to be tested on a hemagg...

Embodiment 3

[0031] Prothrombin time assay kit, including prothrombin time assay reagent, prothrombin time assay reagent consists of buffer system and thromboplastin dissolved in the buffer system, thromboplastin includes rabbit brain powder and tissue For factor esterification, the consumption of rabbit brain powder in the buffer system accounts for 2wt% of the buffer weight, and the consumption of tissue factor esterification in the buffer system accounts for 0.5wt% of the buffer weight; the content of each component in the buffer system is : 0.1wt% TAPSO, 0.5wt% glycine, 0.4wt% polyethylene glycol 6000, 1.0wt% sodium sulfate, 0.01wt% sodium azide, 0.2wt% Brij-35, 6mM chlorine Calcium chloride, the rest is water, and the pH value of the buffer system is 7.0.

[0032] The preparation method of the prothrombin time assay kit is the same as in Example 1.

[0033] Apply the test kit obtained in this embodiment to measure the prothrombin time of the plasma to be tested on a hemagglutination ...

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Abstract

The invention discloses a prothrombin time assay kit and a preparation method thereof. The prothrombin time assay kit, which combines a buffer liquid system which contains a plurality of active ingredients with rabbit brain powder and a tissue factor ester, solves the problem that a single-ingredient reagent is incontrollable in sensitivity; and subsequently, a prothrombin time assay reagent, which is high in accuracy, strong in repeatability and good in stability, and is applicable to various semiautomatic / full-automatic coagulometers, is obtained.

Description

technical field [0001] The invention relates to an in vitro diagnostic kit and a preparation method thereof, which are used for measuring the prothrombin time of human plasma samples. More specifically, it is a kit for measuring prothrombin time prepared by using rabbit brain powder and tissue factor esterified product and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Routine coagulation tests in hospital clinical laboratories, that is, four coagulation routines include prothrombin time measurement (PT), partial activated thromboplastin time measurement (APTT), thrombin time measurement (TT), fibrinogen concentration measurement (FIB) It is mainly used for screening and diagnosis of bleeding disorders, examination of thrombotic diseases and prethrombotic state, monitoring of various anticoagulation therapy and preoperative examination. Among them, PT is the main detection index that reflects the exogenous coagulation pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/56G01N33/86
Inventor 马全新吴艳芳
Owner WUHAN KING DIAGNOSTIC TECH CO LTD
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