Hepaplastin test determination kit and preparation method thereof

A technology for hepatic thromboplastin and a kit, which is applied in the biological field, can solve the problems of widely different results, short storage time, poor stability and repeatability, etc., and achieves good repeatability and stability, high accuracy, and enhanced stability. sexual effect

Inactive Publication Date: 2016-03-02
WUHAN KING DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the different quality of HPT reagents used in the laboratory, the results of the same patient in different hospitals are very different, resulting in inconsistent test results and affecting the correct and timely diagnosis of the disease.
At the same time, the existing HPT reagents have the problems of poor stability and repeatability, and short storage time. Therefore, it is necessary to develop a safe, reliable, accurate, stable, and long-term storage HPT reagent for clinical diagnosis of diseases.

Method used

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  • Hepaplastin test determination kit and preparation method thereof

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Embodiment 1

[0031]Hepatic thromboplastin assay kit, including barium sulfate adsorbed plasma and rabbit brain powder extract reagents, the two reagents are stored in different containers, barium sulfate adsorbed plasma is composed of buffer system a and deficient coagulation factors VII, II, The bovine plasma of X and IX is mixed according to the volume ratio of 1:1. The rabbit brain powder extract reagent is composed of buffer system b, rabbit brain powder dissolved in buffer system b, and tissue factor esterification. The buffer system The content of each component in a is: the MPOS of 0.08wt%, the glycine of 4.5wt%, the Kathon of 0.05wt%, the rest are water, the content of rabbit brain powder is 7.6wt%, the content of tissue factor esterification product is 0.12wt %, the content of each component in buffer system b is: the TAPSO of 0.53wt%, the glycine of 1.7wt%, the polyethylene glycol 6000 of 0.92wt%, the sodium sulfate of 0.43wt%, the calcium chloride of 17mM, the calcium chloride of...

Embodiment 2

[0035] Hepatic thromboplastin assay kit, including barium sulfate adsorbed plasma and rabbit brain powder extract reagents, the two reagents are stored in different containers, barium sulfate adsorbed plasma is composed of buffer system a and deficient coagulation factors VII, II, The plasma of X and IX is mixed according to the volume ratio of 1:9. The blood plasma of deficient coagulation factors VII, II, X, and IX is a mixture of bovine plasma and sheep plasma with a volume ratio of 1:3. Rabbit brain powder extract reagent It is composed of buffer system b, rabbit brain powder and tissue factor esterified product dissolved in buffer system b; the content of each component in buffer system a is: 0.1wt% of TAPSO, 4wt% of glycine, 0.08wt% sodium azide, the rest is water, the content of each component in the buffer system b is: 0.5wt% of TAPSO, 2wt% of glycine, 0.85wt% of polyethylene glycol 6000, 0.5wt% of sodium sulfate, 16mM Calcium chloride, 0.05wt% of Kathon, 0.05wt% of Br...

Embodiment 3

[0039] Hepatic thromboplastin assay kit, including barium sulfate adsorbed plasma and rabbit brain powder extract reagents, the two reagents are stored in different containers, barium sulfate adsorbed plasma is composed of buffer system a and deficient coagulation factors VII, II, The pig plasma of X and IX is mixed according to the volume ratio of 1:4. The rabbit brain powder extract reagent is composed of buffer system b, rabbit brain powder dissolved in buffer system b, and tissue factor esterification. The buffer system The content of each component in a is: 0.01wt% HEPES, 5wt% glycine, 0.01wt% bovine serum albumin, and the rest is water. The content of each component in the buffer system b is: 0.6wt% TAPSO , 1.0wt% glycine, 1.0wt% polyethylene glycol 6000, 0.4wt% sodium sulfate, 20mM calcium chloride, 0.01wt% Proclin, 0.2wt% Brij-35, the rest is water, buffer liquid The pH value of system b is 6.0, the amount of rabbit brain powder in buffer system b accounts for 8wt% of ...

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Abstract

The invention discloses a hepaplastin test determination kit and a preparation method thereof. Through combination of a buffer solution system a containing multiple effective components, plasma without blood coagulation factors VII, II, X and IX, a buffer system b, rabbit brain powder and tissue factor ester, the hepaplastin test determination kit improves reagent stability. A hepaplastin test determination reagent with good accuracy, good repeatability and good effects is further obtained.

Description

technical field [0001] The invention relates to an in vitro diagnostic kit and a preparation method thereof, in particular to a hepatic thromboplastin test kit and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Abnormal liver function refers to that when the liver is damaged by certain pathogenic factors, it can cause the destruction of liver morphology and abnormal metabolism of liver function. If the damage is serious and extensive (one-time or long-term repeated damage), it will cause obvious abnormal changes in liver function such as material metabolism disorder, detoxification function reduction, bile formation and excretion disorder, and bleeding tendency, which is called abnormal liver function. [0003] Due to the variety of liver functions, there are many methods for liver function tests. Protein tests related to liver function include serum total protein, ratio of albumin to globulin, serum turbidity and floc t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573
Inventor 马全新吴艳芳
Owner WUHAN KING DIAGNOSTIC TECH CO LTD
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