Fibrinogen detection reagent/freeze-dried detection reagent for completely inhibiting function of platelets and preparation method of fibrinogen detection reagent/ freeze-dried detection reagent

Abstract

The invention relates to a fibrinogen detection reagent / freeze-dried detection reagent for completely inhibiting the function of platelets and a preparation method of the fibrinogen detection reagent / freeze-dried detection reagent. The detection reagent comprises a buffer solution, a supporting agent, rabbit brain congealed fat, a tissue factor, a platelet inhibitor, a platelet inhibiting enhancer, an organic solvent and a biological preservative; the freeze-dried detection reagent is a quantitative bottled freeze-dried product of the above detection reagent. According to the preparation method of the detection reagent, a buffer solution, a supporting liquid, a rabbit brain congealed fat liquid, a tissue factor liquid, a platelet inhibiting liquid, a platelet inhibiting enhancing liquid and the like are prepared; the liquids are mixed, and a biological preservative is added into an obtained mixture; and the supporting liquid is added to a newly obtained mixture in a full-dose manner. According to the preparation method of the freeze-dried detection reagent, the detection reagent is quantitatively bottled and frozen to be dried. According to the detection reagent / freeze-dried detection reagent provided by the invention, a thrombelastogram detection method is adopted, the function of platelets can be completely inhibited with no additional fibrinogen activators required; the false positive result of fibrinogens can be avoided; the accuracy and stability of detection can be ensured; a process can be simplified; and reagents can be saved.

Description

technical field [0001] The invention relates to a fibrinogen detection reagent and a preparation method thereof, in particular to a fibrinogen detection reagent / lyophilized detection reagent which completely inhibits platelet function and a preparation method thereof, belonging to the technical field of medical device manufacturing. Background technique [0002] Fibrinogen (English: Fibrinogen; abbreviation: FIB), also known as coagulation factor I, is a protein component synthesized by the liver, present in plasma, and involved in the process of platelet coagulation. [0003] It is currently believed that fibrinogen is an important factor in the process of platelet coagulation. [0004] Fibrinogen can promote the aggregation of platelets, the growth, proliferation and contraction of smooth muscle and endothelial cells, increase blood viscosity and peripheral resistance, cause endothelial cell damage, promote the synthesis of collagen and deoxyribonucleic acid, and promote t...

AI Technical Summary

Problems solved by technology

[0008] (3) Fibrinogen is elevated, blood is in a hypercoagulable state, blood flow velocity is slowed down, blood viscosity is increased, and thrombus is prone to occur;

Because platelet inhibitors in the detection reagents, such as atezimumab, eptifibatide, and tirofiban, inhibit platelets, the process is reversible and cannot inhibit all platelets. Therefore, the test results are falsely increased. False-positive results for fibrinogen can occur

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