Brain extract and its prepn and use

A technology of extract and brain tissue, which is applied in the field of biological preparations, can solve problems such as adverse reactions, achieve the effects of relieving the disease, improving neuron metabolism, and being easy to use

Inactive Publication Date: 2006-05-17
诺氏制药(吉林)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to some inconveniences in the application of injections, there are also many adverse reactions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Take 10Kg of fresh and healthy pig brain, add 10L water for injection, homogenize with a high-pressure homogenization pump (GRB1500-4S, Shanghai Donghua high-pressure homogenization), freeze at -35°C, and heat it after freezing at 15°C Keep it at 80°C for 15 minutes, then naturally cool down to room temperature, centrifuge at 10,000 rpm at 4°C in a high-speed refrigerated centrifuge for 30 minutes, then separate the supernatant and the precipitate, and then use the supernatant for use;

[0042] Add 0.5% trypsin [Trypsin, 1:250, Amresco 0458] of the precipitate weight to the precipitate, enzymatically hydrolyze at 37°C for 4 hours, and control the pH of enzymatic hydrolysis to 8.0, then add the aforementioned supernatant to adjust the pH to 3.5. Heat to 80°C, keep it for 15 minutes, then naturally cool to room temperature, centrifuge at 4°C at 4000 rpm for 25 minutes in a low-speed refrigerated centrifuge, then filter the supernatant and adjust the pH of the filtrate 7.0, he...

Embodiment 2

[0044] Take 10Kg of fresh and healthy dog ​​brain, add 10L water for injection, homogenize it under high pressure, freeze at -35℃, after freezing at 20℃, heat to 85℃, keep it for 15 minutes, and then naturally cool to room temperature at 1 Centrifuge in a high-speed refrigerated centrifuge for 25 minutes at a speed of 10,000 rpm at ℃, then separate the supernatant and the precipitate, and then use the supernatant for use;

[0045] Add 0.3% trypsin [Trypsin, 1:250, Amresco 0458] of the precipitate weight to the precipitate, enzymatically hydrolyze at 40°C for 4 hours, and control the pH of enzymatic hydrolysis to 8.0, then add the supernatant to adjust the pH to 3.0, heat to 85°C, keep for 20 minutes, then naturally cool to room temperature, centrifuge at 3500 rpm at 2°C in a low-speed refrigerated centrifuge for 30 minutes, then filter the supernatant and adjust the pH of the filtrate 7.0, heat to 85°C, keep it for 30 minutes, then naturally cool down to room temperature, centrifu...

Embodiment 3

[0047] Take 10Kg of fresh and healthy cow brain, add 15L water for injection, homogenize it under high pressure, freeze at -30℃, and after freezing at 30℃, heat it to 90℃ for 30 minutes, and then naturally cool to room temperature at 2 Centrifuge on a high-speed refrigerated centrifuge for 20 minutes at a speed of 11000 rpm at ℃, then separate the supernatant and the precipitate, and then use the supernatant for use;

[0048] Add 0.1% trypsin [Trypsin, 1:250, Amresco 0458] by weight of the precipitate to the precipitate, enzymatically hydrolyze at 45°C for 6 hours, and control the pH of enzymatic hydrolysis to 7.5, then add the aforementioned supernatant to adjust the pH to 3.5. Heat to 90°C, keep it for 25 minutes, then cool to room temperature naturally, centrifuge at 1°C at 3500 rpm for 25 minutes in a low-speed refrigerated centrifuge, then filter the supernatant and adjust the pH of the filtrate 7.5. Heat to 90°C, keep it for 20 minutes, then naturally cool down to room tempe...

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Abstract

The present invention provides one kind of brain extract extracted from brain tissue of mammal, including pig, dog, ox, sheep, deer, horse and rabbit. The brain extract contains polypeptide, free lysine, free glutamic acid and total sugar in the weight ratio of 1-200 to 0.1-100 to 0.1-80 to 0.1-12, and has molecular weight of 5,000-10,000. The brain extract contains active matters capable of regulating and improving cerebral metabolism and may be used in treating cerebral vascular diseases and corresponding cerebral metabolic disturbance sequela.

Description

Technical field [0001] The present invention relates to a brain extract and its preparation method and application. Specifically, it relates to a brain extract containing polypeptides, free amino acids and total sugars extracted from mammalian brain tissues other than humans, and The preparation method and use of the extract belong to the field of biological preparations. Background technique [0002] The content of free amino acids in brain tissue is highest with glutamic acid, followed by taurine, and then aspartic acid. Glutamate is the main excitatory neurotransmitter in the brain. Brain injury may lead to an imbalance of brain tissue ions, cause an excitotoxic cascade related to glutamate and other excitatory amino acids, and cause the death of neurons in the brain tissue around the initial injury site. Lysine is one of the eight essential amino acids. It plays an important role in the metabolism of the organism. The lack of lysine can cause protein dysfunction, affect growt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K9/14A61K9/16A61K9/19A61K9/20A61K9/48A61K35/30A61P9/10A61P25/00
Inventor 杨尚华
Owner 诺氏制药(吉林)有限公司
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