Ruminant staphylococcus separation, bacteria propagation and capsular polysaccharide expression culture medium
A technology for staphylococci and ruminants, applied in the direction of bacteria, microorganisms, and methods based on microorganisms, etc., can solve the problems that the medium cannot meet the technical requirements, the virulence cannot be restored, and the virulence of the virus has decreased, so as to maintain the virulence of the passage Strong ability, strain preservation, and the effect of maintaining virulence
Inactive Publication Date: 2007-10-31
新疆维吾尔自治区畜牧科学院兽医研究所
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Problems solved by technology
The defect of these mediums is that after staphylococci are isolated, the capsular polysaccharide as the main immunogen and virulence factor is expressed at a low concentration or even not expressed, and disappears after 2-4 generations of continuous passage, resulting in a rapid decline in the virulence of pathogenic bacteria, even if Virulence cannot be restored by returning this animal
However, in the process of production and scientific research, a culture medium that can still maintain its virulence after isolation, enrichment and continuous passage of staphylococci is required, which can provide support for strain preservation, passage, vaccine protection test, and capsular polysaccharide extraction. Therefore, currently The culture medium does not meet the technical requirements
Method used
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[0016] A: Weigh 100g of fresh rabbit brain and 50g of rabbit heart, cut into pieces, grind and homogenate, soak the tissues in 250ml of double-distilled water at 4°C for 48 hours, boil for 15 minutes, filter through filter paper, and add water to 500ml.
[0017] B: Weigh 5g of hydrolyzed milk protein, Peptone 5g, tryptone 5g, soybean peptone 5g, lactose 10g, sodium chloride 30g, dipotassium hydrogen phosphate 5g, magnesium sulfate 0.1g, ferrous sulfate 0.01g, L-cysteine hydrochloride 0.1g, in order Add 400ml of double-distilled water, stir well, and heat to melt.
[0018] C: Mix the above two items of A and B, add water to 1000ml, aliquot, sterilize at 115°C for 15 minutes, and store at 4°C.
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The invention discloses a separating, enriched and capsular polysaccharide expressing medium of ruminant staphylococcus, which comprises the following steps: choosing milky protein hydrolysate, protease leather, tryptone, soy peptone, lactin, common salt, dipotassium hydrogen phosphate, magnesium sulfate, ferrosi sulfas, L-cysteine hydrochlorate, rabbit brain heart drench and double distilled water as raw material; shearing fresh rabbit brain and heart; grinding; homogenizing; soaking with double distilled water under 4 deg. c; boiling for 15 min; filtering with filter paper; compensating water; getting the rabbit brain heart drench. This invention possesses strong ability of bacterial maintain and passage toxic force, which is fit for separating staphylococcus.
Description
1. Technical field [0001] The invention relates to a bacterial culture medium, in particular to a ruminant staphylococcus isolation, enrichment and capsular polysaccharide expression culture medium. 2. Background technology [0002] Staphylococcus is widely distributed in nature, and pathogenic Staphylococcus often causes various suppurative diseases, sepsis or sepsis in humans and animals. Among them, Staphylococcus aureus, Staphylococcus mimicus and other pathogenic bacteria are common pathogens. Traditional vaccines prepared by using live or inactivated bacterial vaccines, isolated peptide sugars, toxoids, adhesins, etc. have no significant effect on the prevention and treatment of staphylococcal infections. The reason for the ineffectiveness of traditional vaccines is that most pathogenic strains have a capsule (mucus layer / microcapsule). Capsular polysaccharides have anti-phagocytic activity and prevent neutrophils from recognizing antibodies to staphylococcal cell wa...
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IPC IPC(8): C12N1/20C12R1/44
Inventor 马文戈黄炯薛英王延
Owner 新疆维吾尔自治区畜牧科学院兽医研究所
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