High-yield bacterial cellulose fermentation medium and fermentation method of bacterial cellulose

A technology of bacterial cellulose and fermentation medium, which is applied in the field of bacterial cellulose high-yield fermentation medium and bacterial cellulose fermentation, and can solve the problems of low bacterial cellulose production

Inactive Publication Date: 2012-12-19
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a high-yield fermentation medium for bacterial cellulose and a fermentat

Method used

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  • High-yield bacterial cellulose fermentation medium and fermentation method of bacterial cellulose
  • High-yield bacterial cellulose fermentation medium and fermentation method of bacterial cellulose
  • High-yield bacterial cellulose fermentation medium and fermentation method of bacterial cellulose

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specific Embodiment approach 1

[0017] Specific embodiment one: the bacterial cellulose high-yield fermentation culture medium of this embodiment consists of 3.5%~4.5% glucose, 0.30%~0.36% beef extract, 0.18%~0.22% yeast extract, 0.21%~0.23% Disodium hydrogen phosphate, 0.454%-0.458% dipotassium hydrogen phosphate, 2.22%-2.23% ethanol, 0.15%-0.25% sodium citrate and the rest distilled water.

[0018] The bacterial cellulose high-yield fermentation medium in this embodiment reduces the flow rate of the HMP pathway, which is beneficial to the synthesis of bacterial cellulose; at the Acetate node, the waste caused by the invalid carbon cycle entering the TCA cycle is reduced, so that more metabolic flow enters the Bacterial cellulose synthesis pathway.

[0019] The addition of ethanol and lactic acid in the bacterial cellulose high-yield fermentation medium of this embodiment can quickly oxidize and enter the TCA cycle for energy generation; in addition, due to the rapid oxidation of ethanol, ATP with high ener...

specific Embodiment approach 2

[0021] Specific embodiment two: the bacterial cellulose high-yield fermentation medium of the present embodiment consists of 3.8%~4.2% glucose, 0.32%~0.34% beef extract, 0.19%~0.21% yeast extract, 0.215%~0.225% by weight percentage Disodium hydrogen phosphate, 0.455%-0.457% dipotassium hydrogen phosphate, 2.225-2.226% ethanol, 0.18%-0.22% sodium citrate and the rest distilled water.

specific Embodiment approach 3

[0022] Specific embodiment three: the bacterial cellulose high-yield fermentation medium of the present embodiment consists of 3.92% glucose, 0.332% beef extract, 0.191% yeast extract, 0.218% disodium hydrogen phosphate, 0.456% dihydrogen phosphate Potassium, 2.226% ethanol, 0.2% sodium citrate and the rest distilled water.

[0023] This embodiment works best.

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Abstract

The invention relates to a high-yield bacterial cellulose fermentation medium and a fermentation method of bacterial cellulose and solves the present problem that the yield of bacterial cellulose is low. The fermentation medium consists of glucose, beef extract, yeast extract, disodium hydrogen phosphate, dipotassium hydrogen phosphate, ethanol, sodium citrate and distilled water. The fermentation method comprises the following steps: 1) activating strains; 2) culturing seeds; and 3) performing fermentation culture. By adopting the technology of the invention, the yield of bacterial cellulose is greatly increased and the average increase of the yield is more than 80%. The high-yield bacterial cellulose fermentation medium and the fermentation method of bacterial cellulose can be used in the production field of bacterial cellulose.

Description

technical field [0001] The invention relates to a bacterial cellulose fermentation medium and a bacterial cellulose fermentation method. Background technique [0002] Bacterial Cellulose (BC) is a kind of pure cellulose produced by microorganisms. It is a high molecular polymer composed of glucose monomer molecules polymerized by β-1,4-glycosidic bonds. Compared with plant fibers, BC does not contain lignin and hemicellulose, has excellent biocompatibility, biocompatibility, biocompatibility and good biodegradability, and is recognized as a safe and excellent performance fiber in the world. Novel biomaterials. It has broad application prospects in the fields of food, papermaking, acoustic equipment, oil exploration, artificial skin, and medical materials. [0003] Although BC has wide application prospects due to its superior performance, the low yield and high added value are the "bottlenecks" that limit its further application, which is determined by the metabolic charac...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01
Inventor 雷虹李元敬张基亮陈雪梅田华平文祥韩德权
Owner HEILONGJIANG UNIV
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