Fermentation medium for extracting astragalus polysaccharides

A fermentation medium, astragalus polysaccharide technology, applied in microorganism-based methods, microorganisms, bacteria and other directions, can solve the problems of no extraction rate reference data, low utilization rate of Chinese medicinal materials, and unstable extraction, and achieve good extraction rate and effect, beneficial to proliferation, and the effect of broad practical application prospects

Active Publication Date: 2012-08-22
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional water extraction and alcohol precipitation method is the most commonly used extraction method at present, and the extraction rate of astragalus polysaccharide is about 5.34% on average; the ultrafine pulverization method, the extraction rate of astragalus polysaccharide in the superfine powder is about 4.69%, and the extraction rate of astragalus polysaccharide in the coarse powder is about 4.69%. The extraction rate is only about 2.16%; the extraction rate of astragalus polysaccharide by ultrasonic cell crushing method is about 7.6%; the extraction rate of astragalus polysaccharide by microwave extraction method is about 6.55%; the extraction rate of astragalus polysaccharide by ultrafiltration is lower than that by water extraction and alcohol precipitation method , but

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) The fermentation medium for extracting astragalus polysaccharides is prepared from the following components

[0028] According to whey powder: 5.158 g; peptone: 0.456 g; glucose: 0.12 g; yeast powder: 0.312 g; inorganic salt (potassium dihydrogen phosphate and dipotassium hydrogen phosphate): 0.194 g; astragalus powder: 16 g; water: 200 The weight ratio in ml weighed each component, added to a 500ml Erlenmeyer flask, sterilized in a pressure cooker at 121°C for 20min, and adjusted the pH to 6.9 for later use.

[0029] (2) Expanded cultivation of fermentation strains

[0030] (1) Pass the fermentation strain LZMYFGM9 on the slant of MRS medium, and the fermentation conditions are (the inoculum size is 10 3 cfu):

[0031] Fermentation time: 16-20 hours;

[0032] Fermentation environment: anaerobic fermentation;

[0033] Fermentation temperature: 36.5-37.5°C;

[0034] Initial pH: 6.0-6.4.

[0035] (2) Then, insert it into the primary or secondary seed medium, and...

Embodiment 2

[0048] The fermentation medium for extracting astragalus polysaccharide is prepared from the following components:

[0049] According to whey powder: 38.69 g; peptone: 3.42 g; glucose: 0.9 g; yeast powder: 2.34 g; inorganic salt (potassium dihydrogen phosphate and dipotassium hydrogen phosphate): 1.455 g; astragalus powder: 120 g; The weight ratio of L Weigh each component, put it into a 10L anaerobic fermentation tank, sterilize at 121°C for 20 minutes, and adjust the pH to 6.9 for later use.

[0050] All the other steps are the same as in Example 1.

[0051] The total weight of the obtained extract is 28.68g, the extraction rate of astragalus polysaccharide is 23.90%, and the content of astragalus polysaccharide is 52.78%.

Embodiment 3

[0053] According to whey powder: 128.95 g; peptone: 11.4 g; glucose: 3.0 g; yeast powder: 7.8 g; inorganic salt (potassium dihydrogen phosphate and dipotassium hydrogen phosphate): 4.85 g; astragalus powder: 400 g; water: 5 The weight ratio of L Weigh each component, add it to a 10L anaerobic fermentation tank, sterilize at 121°C for 20 minutes, adjust the pH to 6.9 for later use.

[0054] All the other steps are the same as in Example 1.

[0055] The total weight of the obtained extract was 78.04g, the extraction rate of astragalus polysaccharide was 19.51%, and the content of astragalus polysaccharide was 41.20%.

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Abstract

The invention discloses a fermentation medium for exracting astragalus polysaccharides. The fermentation medium is prepared from the following compositions in parts by weight: 3.908-6.408 parts of whey powder, 0.445-0.467 parts of peptone, 0.04-0.2 parts of glucose, 0.102-0.522 parts of yeast powder, 0.142-0.246 parts of inorganic salt, 12.54-19.46 parts of astragalus powder and 200 parts of water, wherein the inorganic salt comprises potassium dihydrogen phosphate and dipotassium hydrogen phosphate. The fermentation medium for exracting astragalus polysaccharides, disclosed by the invention, can be used for obviously and stably improving the extraction ratio of astragalus polysaccharides and ensuring that the content of astragalus polysaccharides in extraction products is obviously improved.

Description

technical field [0001] The invention relates to a bacterial culture medium, in particular to a fermentation medium for extracting astragalus polysaccharide. Background technique [0002] Astragalus polysaccharide is the active ingredient of traditional Chinese medicine Astragalus. It is a plant polysaccharide with a molecular weight of about 37,500 connected by α-glucoside bonds. After hydrolysis, glucose, lactose and arabinose can be detected. The molar ratio of the three sugars is: 1:0.95 :0.70. Astragalus polysaccharide is an effective immune enhancer, and can induce the proliferation of probiotics in the body and promote the micro-ecological balance of the body. Research reports have shown that astragalus polysaccharides also have health benefits such as enhancing cell metabolism, promoting blood circulation, anti-virus, anti-tumor, and anti-stress, and have been widely used as functional ingredients or taken directly. [0003] At present, the astragalus polysaccharide...

Claims

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Application Information

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IPC IPC(8): C12N1/20C08B37/00C12R1/46
Inventor 张凯李建喜张景艳孟嘉仁杨志强王学智
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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