Fermentation medium for extracting astragalus polysaccharides
A fermentation medium, astragalus polysaccharide technology, applied in microorganism-based methods, microorganisms, bacteria and other directions, can solve the problems of no extraction rate reference data, low utilization rate of Chinese medicinal materials, and unstable extraction, and achieve good extraction rate and effect, beneficial to proliferation, and the effect of broad practical application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0027] (1) The fermentation medium for extracting astragalus polysaccharides is prepared from the following components
[0028] According to whey powder: 5.158 g; peptone: 0.456 g; glucose: 0.12 g; yeast powder: 0.312 g; inorganic salt (potassium dihydrogen phosphate and dipotassium hydrogen phosphate): 0.194 g; astragalus powder: 16 g; water: 200 The weight ratio in ml weighed each component, added to a 500ml Erlenmeyer flask, sterilized in a pressure cooker at 121°C for 20min, and adjusted the pH to 6.9 for later use.
[0029] (2) Expanded cultivation of fermentation strains
[0030] (1) Pass the fermentation strain LZMYFGM9 on the slant of MRS medium, and the fermentation conditions are (the inoculum size is 10 3 cfu):
[0031] Fermentation time: 16-20 hours;
[0032] Fermentation environment: anaerobic fermentation;
[0033] Fermentation temperature: 36.5-37.5°C;
[0034] Initial pH: 6.0-6.4.
[0035] (2) Then, insert it into the primary or secondary seed medium, and...
Embodiment 2
[0048] The fermentation medium for extracting astragalus polysaccharide is prepared from the following components:
[0049] According to whey powder: 38.69 g; peptone: 3.42 g; glucose: 0.9 g; yeast powder: 2.34 g; inorganic salt (potassium dihydrogen phosphate and dipotassium hydrogen phosphate): 1.455 g; astragalus powder: 120 g; The weight ratio of L Weigh each component, put it into a 10L anaerobic fermentation tank, sterilize at 121°C for 20 minutes, and adjust the pH to 6.9 for later use.
[0050] All the other steps are the same as in Example 1.
[0051] The total weight of the obtained extract is 28.68g, the extraction rate of astragalus polysaccharide is 23.90%, and the content of astragalus polysaccharide is 52.78%.
Embodiment 3
[0053] According to whey powder: 128.95 g; peptone: 11.4 g; glucose: 3.0 g; yeast powder: 7.8 g; inorganic salt (potassium dihydrogen phosphate and dipotassium hydrogen phosphate): 4.85 g; astragalus powder: 400 g; water: 5 The weight ratio of L Weigh each component, add it to a 10L anaerobic fermentation tank, sterilize at 121°C for 20 minutes, adjust the pH to 6.9 for later use.
[0054] All the other steps are the same as in Example 1.
[0055] The total weight of the obtained extract was 78.04g, the extraction rate of astragalus polysaccharide was 19.51%, and the content of astragalus polysaccharide was 41.20%.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com