Biological detoxification method for camellia seed cakes

A tea seed cake and biological technology, applied in the field of biological detoxification, can solve the problems of increasing feed cost and loss of nutritional components, and achieve the effects of small investment, no three wastes, and poor palatability

Inactive Publication Date: 2012-06-13
林元山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From this point of view, although physical and chemical solvent leaching of tea saponin can reduce saponin, when the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The strains used were 1 strain of Lactobacillus delbrueckii, 1 strain of Bacillus subtilis, and 1 strain of Saccharomyces cerevisiae. The steps are:

[0022] (1) Preparation of liquid strains: take protein 5.0 g, beef extract 5.0 g, yeast powder 4.0 g, glucose 10.0 g, Tween 80 0.5 mL, dipotassium hydrogen phosphate 1.0 g, sodium acetate 3.0 g, triammonium citrate 2.0 g of magnesium chloride, 0.2 g of magnesium chloride, 0.05 g of manganese sulfate, and 1000 mL of distilled water were mixed evenly, and then sterilized at 115 °C for 15 minutes to form a Lactobacillus liquid culture medium, cooled to room temperature, and then connected to cultivated mature slant bacteria were incubated at 41 °C in an anaerobic shaker for 12 h; 10.0 g of glucose, 5.0 g of peptone, 3.0 g of beef extract, 3.0 g of sodium chloride, and 1000 mL of water were mixed, and sterilized under 0.1 MPa pressure for 25 min, cooled to room temperature, and then inserted into the cultured Bacillus subtil...

Embodiment 2

[0026] The strains used were 1 strain of Lactobacillus delbrueckii, 1 strain of Bacillus subtilis, and 1 strain of Saccharomyces cerevisiae. The steps are:

[0027] (1) Preparation of liquid strains: take protein 7.0 g, beef extract 7.0 g, yeast powder 5.0 g, glucose 10.0 g, Tween 80 0.8 mL, dipotassium hydrogen phosphate 1.0 g, sodium acetate 3.0 g, triammonium citrate 2.0 g of magnesium chloride, 0.3 g of magnesium chloride, 0.06 g of manganese sulfate, and 1000 mL of distilled water were mixed evenly, and then sterilized at 115 °C for 20 min to form a Lactobacillus liquid culture medium. After cooling to room temperature, the cultured mature slant bacteria was added. 15.0 g of glucose, 6.0 g of peptone, 4.0 g of beef extract, 4.0 g of sodium chloride and 1000 mL of water were mixed and sterilized under 0.1 MPa pressure. After cooling to room temperature for 25 min, the cultured Bacillus subtilis slant strain was inserted, and incubated at 37 °C and 200 r / min for 20 h on a ...

Embodiment 3

[0031] The strains used were 1 strain of Lactobacillus delbrueckii, 1 strain of Bacillus subtilis, and 1 strain of Saccharomyces cerevisiae. The steps are:

[0032] (1) Preparation of liquid strains: take protein 10.0 g, beef extract 5.0 g, yeast powder 3.0 g, glucose 12.0 g, Tween 80 0.5 mL, dipotassium hydrogen phosphate 1.0 g, sodium acetate 3.0 g, triammonium citrate 2.0 g of magnesium chloride, 0.2 g of magnesium chloride, 0.05 g of manganese sulfate, and 1000 mL of distilled water were mixed evenly, and then sterilized at 115 °C for 15 minutes to form a Lactobacillus liquid culture medium. 12.0 g of glucose, 5.0 g of peptone, 3.0 g of beef extract, 3.0 g of sodium chloride, and 1000 mL of water were mixed well, and sterilized under 0.1 MPa pressure. After cooling to room temperature for 25 min, the cultured Bacillus subtilis slant strain was inserted, and incubated at 35 °C and 200 r / min for 24 h on a shaker; 60.0 g of glucose, 4.0 g of yeast extract, 2.0 g of sodium ch...

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PUM

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Abstract

The invention relates to a biological detoxification method for camellia seed cakes. According to the invention, dipotassium hydrogen phosphate, magnesium chloride, 2000-4000U/g of acidic cellulase, microbe strains, and the like are added to crushed camellia seed cake; the mixture is well mixed by stirring, and is subject to a reaction for 10-15 days under a temperature of 40-50 DEG C under the effects of both the microbes and the acidic cellulase; an obtained material is dried by baking, and is crushed, such that a finished product is obtained. Tea saponin content in the obtained product is lower than a safety index of a feedstuff standard, and the nutritive value of the product is higher than non-fermented camellia seed cakes. Protein content of the product reaches 15-20%, which is improved by more than 5%. Free amino acid content of the product is higher than 5%, which is improved by more than 25%. Reducing sugar content in the product is higher than 5%, saponin content in the product is lower than 1.0%, and a detoxification rate of the product is higher than 90%. The product can be directly used as a feedstuff, such that a problem of camellia seed cake intoxication in prior art is solved. The production method is advantaged in simple technology and good effect. With the method, non-toxic camellia seed cakes satisfying the requirements of the feedstuff standard can be provided.

Description

technical field [0001] The invention relates to a biological detoxification method combining microorganisms and enzymes of tea seed cakes. Background technique [0002] Camellia oleifera is an important oil crop in the hilly areas of southern my country, accounting for more than 80% of the national woody edible oil crops. Tea seed cake, also known as tea meal, alias tea bran, tea dry, tea seed cake. The purple-brown particles are the residue left after the wild camellia oil fruit is pressed. Tea seed cakes after oil extraction also contain a large amount of starch, protein, crude fat, soluble sugar and tea saponin, etc., which can be re-extracted and utilized. The area of ​​Camellia oleifera forests in my country is about 4 million hectares, and it is the country with the most varieties, the widest distribution and the largest output of Camellia oleifera in the world. According to statistics, the area of ​​tea plantations in my country is about 1 million hectares. In rece...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23L1/015A23L5/20
Inventor 林元山李海翔吴永尧
Owner 林元山
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