Biological detoxification method for camellia seed cakes
A tea seed cake and biological technology, applied in the field of biological detoxification, can solve the problems of increasing feed cost and loss of nutritional components, and achieve the effects of small investment, no three wastes, and poor palatability
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Embodiment 1
[0021] The strains used were 1 strain of Lactobacillus delbrueckii, 1 strain of Bacillus subtilis, and 1 strain of Saccharomyces cerevisiae. The steps are:
[0022] (1) Preparation of liquid strains: take protein 5.0 g, beef extract 5.0 g, yeast powder 4.0 g, glucose 10.0 g, Tween 80 0.5 mL, dipotassium hydrogen phosphate 1.0 g, sodium acetate 3.0 g, triammonium citrate 2.0 g of magnesium chloride, 0.2 g of magnesium chloride, 0.05 g of manganese sulfate, and 1000 mL of distilled water were mixed evenly, and then sterilized at 115 °C for 15 minutes to form a Lactobacillus liquid culture medium, cooled to room temperature, and then connected to cultivated mature slant bacteria were incubated at 41 °C in an anaerobic shaker for 12 h; 10.0 g of glucose, 5.0 g of peptone, 3.0 g of beef extract, 3.0 g of sodium chloride, and 1000 mL of water were mixed, and sterilized under 0.1 MPa pressure for 25 min, cooled to room temperature, and then inserted into the cultured Bacillus subtil...
Embodiment 2
[0026] The strains used were 1 strain of Lactobacillus delbrueckii, 1 strain of Bacillus subtilis, and 1 strain of Saccharomyces cerevisiae. The steps are:
[0027] (1) Preparation of liquid strains: take protein 7.0 g, beef extract 7.0 g, yeast powder 5.0 g, glucose 10.0 g, Tween 80 0.8 mL, dipotassium hydrogen phosphate 1.0 g, sodium acetate 3.0 g, triammonium citrate 2.0 g of magnesium chloride, 0.3 g of magnesium chloride, 0.06 g of manganese sulfate, and 1000 mL of distilled water were mixed evenly, and then sterilized at 115 °C for 20 min to form a Lactobacillus liquid culture medium. After cooling to room temperature, the cultured mature slant bacteria was added. 15.0 g of glucose, 6.0 g of peptone, 4.0 g of beef extract, 4.0 g of sodium chloride and 1000 mL of water were mixed and sterilized under 0.1 MPa pressure. After cooling to room temperature for 25 min, the cultured Bacillus subtilis slant strain was inserted, and incubated at 37 °C and 200 r / min for 20 h on a ...
Embodiment 3
[0031] The strains used were 1 strain of Lactobacillus delbrueckii, 1 strain of Bacillus subtilis, and 1 strain of Saccharomyces cerevisiae. The steps are:
[0032] (1) Preparation of liquid strains: take protein 10.0 g, beef extract 5.0 g, yeast powder 3.0 g, glucose 12.0 g, Tween 80 0.5 mL, dipotassium hydrogen phosphate 1.0 g, sodium acetate 3.0 g, triammonium citrate 2.0 g of magnesium chloride, 0.2 g of magnesium chloride, 0.05 g of manganese sulfate, and 1000 mL of distilled water were mixed evenly, and then sterilized at 115 °C for 15 minutes to form a Lactobacillus liquid culture medium. 12.0 g of glucose, 5.0 g of peptone, 3.0 g of beef extract, 3.0 g of sodium chloride, and 1000 mL of water were mixed well, and sterilized under 0.1 MPa pressure. After cooling to room temperature for 25 min, the cultured Bacillus subtilis slant strain was inserted, and incubated at 35 °C and 200 r / min for 24 h on a shaker; 60.0 g of glucose, 4.0 g of yeast extract, 2.0 g of sodium ch...
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