Preparing method for pig thrombin freeze-dried powder

A technology for porcine thrombin and freeze-dried powder, which is applied in the field of preparation of porcine thrombin freeze-dried powder, can solve the problems of unavoidable secondary contamination risk of exogenous virus, complex prothrombin activation method, and many process steps, etc. Product safety, improving product safety, and the effect of simple process steps
CN105250226AActive Publication Date: 2016-01-20WUHAN HITECK BIOLOGICAL PHARMA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN HITECK BIOLOGICAL PHARMA
Publication Date
2016-01-20

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Abstract

The invention discloses a preparing method for pig thrombin freeze-dried powder. The preparing method comprises the following steps that anticoagulant pig plasma and gel are mixed and stirred for adsorption and pass through a column for elution, and a prothrombin solution is obtained; normal saline is added to rabbit brain powder, then the prothrombin solution and CaCl2 are added for zymogen activation, a crude thrombin enzyme solution is obtained, subjected to ultrafiltration for desalination and concentrated, viral inactivation is carried out, an obtained crude enzyme solution passes through DEAE-Sepharose Fast Flow chromatographic column, elution is carried out, target peaks are collected, and pig thrombin is obtained; the pig thrombin is added with mannitol or dextran 40, filtering, sterilizing, subpackaging, freeze drying and vacuum tamponing are carried out, an aluminum plastic combined cover is rolled, and a pig thrombin freeze-dried product is obtained; the freeze-dried product is subjected to dry heat treatment at 100 DEG C for secondary virus inactivation, and the pig thrombin freeze-dried powder is obtained after packaging. The specific activity of pig thrombin in the product is not lower than 130 U / mg, the whole process is simple in step and easy to implement, the product safety is improved, and the preparing method is suitable for industrial production.
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Description

technical field

[0001] The invention relates to a preparation process of porcine thrombin freeze-dried powder, in particular to a preparation method of porcine thrombin freeze-dried powder. Background technique

[0002] Thrombin is a serine proteolytic enzyme that plays an important role in the blood coagulation system. It can hydrolyze the 4 Arg-Gly peptide bonds of fibrinogen, transform the fibrinogen in the sol state into fibrin in the gel state, and promote Blood coagulation can also promote platelet aggregation, further accelerate blood coagulation, and achieve the purpose of rapid hemostasis.

[0003] At present, most of the thrombin preparations used clinically are prothrombin extracted from bovine blood or pig blood, which is activated by prothrombin activator to obtain a sterile freeze-dried product of thrombin, which has high specificity. It is a fast-acting local hemostatic drug, which is widely used in gastrointestinal bleeding and surgical hemostasis. (Song Ho...

Claims

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