A kind of ellagic acid reagent and its preparation method and activated partial thromboplastin time determination reagent, aptt kit

Abstract

The invention provides an ellagic acid reagent and a preparation method thereof as well as an activated partial thromboplastin time (APTT) determination reagent and an APTT kit and relates to the field of clinical diagnosis reagents. The preparation method comprises the following steps: adding ellagic acid into calcium-free purified water and rapidly stirring to uniformly disperse the ellagic acid; adding an alkaline buffering solution or inorganic alkali; rapidly adding an acidic buffering solution or organic acid and inorganic acid within 10min after the ellagic acid is added; adding metal ions; adding amino acid and a derivative thereof to obtain a buffering solution; furthermore, adding the calcium-free purified water into rabbit brain phospholipid and uniformly mixing and emulsifyingto obtain emulsified phospholipid; adding the emulsified phospholipid into the buffering solution and rapidly stirring and uniformly mixing to obtain the ellagic acid reagent. The technology is simpleand rapid and has detailed descriptions. The activated partial thromboplastin time determination reagent comprises the ellagic acid reagent and a calcium chloride reagent and has good stability. TheAPTT kit containing the activated partial thromboplastin time determination reagent can be used for directly replacing an imported high-stability APTT kit.

Description

technical field [0001] The invention relates to the field of clinical diagnostic reagents, and in particular to an ellagic acid reagent and a preparation method thereof, a reagent for measuring activated partial thromboplastin time, and an APTT kit. Background technique [0002] In the field of clinical examinations, it is known to measure blood coagulation time to study abnormalities of blood coagulation factors, among which APTT measurement is one of general clinical examinations of blood coagulation ability. APTT (activated partial thromboplastin time), namely activated partial thromboplastin time, refers to the clotting time that reflects the function of the intrinsic coagulation pathway that blood starts through contact with negatively charged foreign bodies during bleeding. APTT measurement is used not only for screening tests for the deficiency or abnormality of intrinsic coagulation factors but also for monitoring heparin therapy and the like. [0003] Most of the e...

AI Technical Summary

Problems solved by technology

In addition, as the type of synthetic phospholipids, mixing three types of phosphatidylethanolamine (PE), phosphatidylcholine (PC) and phosphatidylserine (PS) into the reagent has become the mainstream, but there are many types of synthetic phospholipids, and it is not easy to Obtained and expensive, in addition, the proportion of each component is difficult to control, and there are also dissolution problems, some of which will lead to precipitation and affect the quality of reagents

Existing reagents will have ellagic acid and phospholipid precipitation problems, ellagic acid buffer solution, raw material treatment such as ellagic acid will lose activity under alkaline conditions, whether the phospholipid emulsification is uniform, and the ratio of ellagic acid buffer solution to phospholipids etc. have directly affected the quality of domestic reagents, resulting in the market being still dominated by giants such as Siemens, Starco, and Werfen.

Images