Method for preparing high-strength double-network hydrogel stent by virtue of 3D printing

Abstract

The invention discloses a method for preparing a high-strength double-network hydrogel stent by virtue of 3D printing. The method comprises the following steps of adding a polymer monomer N, N-dimethyl acrylamide, an initiator, a crosslinking agent and sodium alginate (SA) into deionized water to form a solution and adding inorganic powder hydroxyapatite to obtain a sol; controlling and extruding the sol by a robot dispenser, and carrying out 3D printing molding to obtain a sol stent; placing the sol stent under ultraviolet light so that the monomer in the stent is subjected to photopolymerization and chemical cross-linking reaction to form a layer of chemically cross-linked network pre-molded hydrogel stent; immersing the pre-molded hydrogel stent into a CaCl2 aqueous solution so that SA in the stent is subjected to physical crosslinking to form a second layer of physically cross-linked network so as to obtain the hydrogel stent having physically and chemically cross-linked double-network. The hydrogel stent prepared by the method has higher mechanical strength and fine internal structure, and the three-dimensional morphology of the stent can be conveniently regulated and controlled to adapt to the complex application requirements of tissue engineering materials.

Description

technical field [0001] The invention belongs to the technical field of biomedical polymer materials, and in particular relates to a method for preparing a high-strength double-network hydrogel scaffold by 3D printing. Background technique [0002] In the field of biomedicine, organ and tissue transplantation and repair still face enormous difficulties and challenges. In recent years, 3D printing tissue engineering scaffold technology has played an increasingly important role in solving such problems. 3D printing tissue engineering scaffold technology is an emerging technology that combines three-dimensional rapid prototyping technology and tissue engineering technology to print scaffolds with good biocompatibility, excellent mechanical properties, ideal three-dimensional microstructure and controllable macroscopic shape. The scaffolds printed by this technology can be used for cell culture, allowing cells to grow, proliferate and differentiate in it to form tissues or organ...

AI Technical Summary

Problems solved by technology

Laser cross-linking is performed while the scaffold is being printed. Due to the slow polymer chemical cross-linking reaction in aqueous solution, the accuracy of the printed and simultaneously cross-linked scaffold is limited, generally from hundreds of microns to several millimeters, which is not conducive to construction. Fine and complex tissue scaffold structure

At the same time, due to the different light intensity received by different parts of the sol, the degree of polymerization and crosslinking reactions will also be different, which will affect the uniformity of the properties of the hydrogel.

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