Method for preparing high-strength double-network hydrogel stent by virtue of 3D printing

A 3D printing and hydrogel technology, applied in the fields of medical science, prosthesis, additive processing, etc., can solve the problems that are not conducive to the construction of fine and complex tissue scaffold structures, slow polymer chemical cross-linking reactions, and limitations, generally about several 100 microns to several millimeters, etc., to achieve good mechanical properties, low printing cost, and fine structure

Inactive Publication Date: 2015-05-20
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Laser cross-linking is performed while the scaffold is being printed. Due to the slow polymer chemical cross-linking reaction in aqueous solution, the accuracy of the printed and simultaneously cross-linked scaffold is limited, generally from hundreds of microns to several millimeters, which is not conduc

Method used

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  • Method for preparing high-strength double-network hydrogel stent by virtue of 3D printing
  • Method for preparing high-strength double-network hydrogel stent by virtue of 3D printing
  • Method for preparing high-strength double-network hydrogel stent by virtue of 3D printing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) 1.5g sodium alginate (SA), 15g N,N-dimethylacrylamide (DMAA) polymer monomer, 0.0029g ketoglutaric acid (KA) photoinitiator, 0.0093g N,N′ -Methylenebisacrylamide (MBAA) crosslinking agent and deionized water were mixed evenly to 100ml, and then the above 100ml mixed solution was slowly added to a beaker containing 22.5g of HA with a particle size of 20nm, and stirred evenly. Prepared as a mixed sol;

[0043] (2) Preforming of the hydrogel scaffold: use the model The Loctite ? The robot dispensing machine of 200D Benchtop Robot extrudes the mixed sol prepared in step (1) under the selected technical parameters, and 3D prints out the sol support with fine structure. Among them, the technical parameters selected by the robot dispensing machine are:

[0044] Dispenser extrusion rate (volume flow rate) 0.048ml / min

[0045] XY axis platform moving speed 180mm / min

[0046] Z-axis step height 0.4mm

[0047] Needle diameter 100μm;

[0048] (3) Put the printed sol scaff...

Embodiment 2

[0051] (1) 1.8g sodium alginate (SA), 19.8g N,N-bismethacrylamide (DMAA) polymer monomer, 0.0029g ketoglutaric acid (KA) photoinitiator, 0.0093g N,N ′-Methylenebisacrylamide (MBAA) cross-linking agent and deionized water are mixed evenly to 100ml, and then the above 100ml mixed solution is slowly added to a beaker containing 32.4g of HA with a particle size of 80nm, and stirred evenly , formulated into a mixed sol;

[0052] (2) Preforming of the hydrogel scaffold: using a Sistema Dosificador Ultra 2800 robotic dispensing machine, extrude the prepared mixed sol described in step (1) under the selected technical parameters, and 3D print out a fine The structure of the sol support, in which the technical parameters selected by the robot dispensing machine are:

[0053] Dispenser extrusion rate (volume flow rate) 0.072ml / min

[0054] XY axis platform moving speed 250mm / min

[0055] Z-axis step height 0.6mm

[0056] Needle diameter 200μm;

[0057] (3) Put the printed sol scaff...

Embodiment 3

[0061] (1) 2.0g sodium alginate (SA), 24g N,N-bismethacrylamide (DMAA) polymer monomer, 0.0029g ketoglutaric acid (KA) photoinitiator, 0.0093g N,N′ - Methylenebisacrylamide (MBAA) cross-linking agent and deionized water were mixed evenly to 100ml, and then the above 100ml mixed solution was slowly added to a beaker containing 40g of HA with a particle size of 200nm, stirred evenly, and prepared into a mixed sol;

[0062] (2) Preforming of the hydrogel scaffold: use the model The Loctite ? The robot dispensing machine of 400D Benchtop Robot extrudes the mixed sol prepared in step (1) under the selected technical parameters, and 3D prints out the sol support with fine structure, wherein the technical parameters selected by the robot dispensing machine for:

[0063] Dispenser extrusion rate (volume flow rate) 0.090ml / min

[0064] XY axis platform moving speed 220mm / min

[0065] Z-axis step height 0.5mm

[0066] Needle diameter 500μm;

[0067](3) Place the printed sol s...

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Abstract

The invention discloses a method for preparing a high-strength double-network hydrogel stent by virtue of 3D printing. The method comprises the following steps of adding a polymer monomer N, N-dimethyl acrylamide, an initiator, a crosslinking agent and sodium alginate (SA) into deionized water to form a solution and adding inorganic powder hydroxyapatite to obtain a sol; controlling and extruding the sol by a robot dispenser, and carrying out 3D printing molding to obtain a sol stent; placing the sol stent under ultraviolet light so that the monomer in the stent is subjected to photopolymerization and chemical cross-linking reaction to form a layer of chemically cross-linked network pre-molded hydrogel stent; immersing the pre-molded hydrogel stent into a CaCl2 aqueous solution so that SA in the stent is subjected to physical crosslinking to form a second layer of physically cross-linked network so as to obtain the hydrogel stent having physically and chemically cross-linked double-network. The hydrogel stent prepared by the method has higher mechanical strength and fine internal structure, and the three-dimensional morphology of the stent can be conveniently regulated and controlled to adapt to the complex application requirements of tissue engineering materials.

Description

technical field [0001] The invention belongs to the technical field of biomedical polymer materials, and in particular relates to a method for preparing a high-strength double-network hydrogel scaffold by 3D printing. Background technique [0002] In the field of biomedicine, organ and tissue transplantation and repair still face enormous difficulties and challenges. In recent years, 3D printing tissue engineering scaffold technology has played an increasingly important role in solving such problems. 3D printing tissue engineering scaffold technology is an emerging technology that combines three-dimensional rapid prototyping technology and tissue engineering technology to print scaffolds with good biocompatibility, excellent mechanical properties, ideal three-dimensional microstructure and controllable macroscopic shape. The scaffolds printed by this technology can be used for cell culture, allowing cells to grow, proliferate and differentiate in it to form tissues or organ...

Claims

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Application Information

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IPC IPC(8): C08F220/54C08F2/48C08J3/24C08L33/24C08L5/04C08K3/32B29C67/24B33Y10/00B33Y70/00A61L27/26A61L27/52
Inventor 李学锋黄大华闫晗袁亚龙世军
Owner HUBEI UNIV OF TECH
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