Bone-repairing hydrogel stent and preparation method thereof

A technology for hydrogel and bone repair, which is applied in the fields of pharmaceutical formulation, medical science, prosthesis, etc. It can solve problems such as unfavorable loading of cells and implantation of the body, limitation of the application range of hydrogel materials, and harsh molding conditions of the molding system. , to achieve good clinical application value, facilitate cell growth and proliferation, and controllable degradation rate

Active Publication Date: 2018-11-16
深圳市晶莱新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the porous hydrogel prepared by the above method will lead to a decrease in elastic modulus and a decrease in mechanical strength with the increase of porosity and pore connectivity, which limits it to the design of soft tissue regeneration and repair, thus limiting Applications of hydrogel materials
Secondly, because of the comp

Method used

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  • Bone-repairing hydrogel stent and preparation method thereof
  • Bone-repairing hydrogel stent and preparation method thereof

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preparation example Construction

[0034] In a second aspect, the present invention provides a method for preparing a hydrogel scaffold for bone repair, comprising the following preparation steps:

[0035] Step S1, preparation of polymer gel carrier material: in parts by weight, dissolve 10-20 parts of polymer gel solution, 1-5 parts of matrix metalloproteinase-responsive peptide chain, and 50-100 parts of photocrosslinking agent In the mixed solution of triethylamine and acetone, the chemical reaction is carried out with magnetic stirring at normal temperature. After the reaction is completed, the reactant is purified, and the purified product is freeze-dried and stored in a refrigerator at -20°C.

[0036] In detail, step S1 is to modify the polymer gel solution, and react the polymer gel solution and the matrix metalloproteinase-responsive peptide chain with a photocrosslinking agent in the environment of triethylamine and acetone, so that the polymer gel The gel solution is connected to the double bond of th...

Embodiment 1

[0051] Step S1: Dissolve 1g of collagen in 0.5M acetic acid solution at a concentration of 5mg / ml, add 0.1g of polypeptide GPLGVRGDG-NH2, and dissolve 50ml of acetone solution containing 10ml of triethylamine and 10ml of glycidyl methacrylate, Stir magnetically at 37°C for 2 days; then add the mixed solution into a dialysis bag, place it in an external environment of ultrapure water, and change the ultrapure water every 6 hours to fully remove residual organic solvents and impurity ions, and perform dialysis After 2 days, the dialysis-purified product was freeze-dried and stored in a refrigerator at -20°C until use;

[0052] Step S2: CaCl at 0.05M, 200ml 2 In the solution, add 20ml 5mg / ml silk fibroin solution, add 120ml, 0.05M Na 2 HPO 4 Solution, and at the same time dropwise add 1M NaOH solution to adjust the pH value between 7-8, place it in a water bath at 37°C and stir for 1 day, then use ultrapure water to filter and wash the precipitate, freeze-dry and grind to obtai...

Embodiment 2

[0055] Step S1: Dissolve 1 g of hyaluronic acid in PBS buffer solution at a concentration of 10 mg / ml, add 0.1 g of polypeptide GPLGVRG-NH2, and 150 ml of acetone solution dissolved in 30 ml of triethylamine and 30 ml of isocyanoethyl methacrylate, Stir magnetically at 37°C for 2 days; then add the mixed solution to 5 times the volume of acetone for precipitation, dissolve it in PBS, repeat this step 3 times to fully remove residual organic solvents and impurity ions, and freeze-dry the purified product , stored in the refrigerator at -20°C for later use;

[0056] Step S2: in 0.05M, 200ml Ca(NO 3 ) 2 In the solution, add 20ml of collagen solution dissolved in 0.5M acetic acid with a concentration of 5mg / ml, and add 120ml of 0.05M (NH 4 ) 2 HPO 4 solution, and at the same time dropwise added 1M NaOH solution to adjust the pH value between 7 and 8, placed in a 37°C water bath and stirred for 2 hours, aged for 2 days, filtered and washed the resulting precipitate with ultrapu...

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Abstract

The invention provides a bone-repairing hydrogel stent and a preparation method thereof. The bone-repairing hydrogel stent comprises a polymer gel carrier and mineralized nano bone particles. The polymer gel carrier comprises polymer gel and matrix metalloprotease responding peptide chains. The matrix metalloprotease responding peptide chains are directly crosslinked to the polymer gel. The bone-repairing hydrogel stent is prepared by 3D printing under the irradiation of UV rays. Furthermore, the invention also relates to a preparation method of the bone-repairing hydrogel stent. The providedbone-repairing hydrogel stent has the advantages of high porosity and mechanical strength, and can intelligently response to mesenchymal stem cells or bone cell systems, promote tissue growth, and induce bone healing. Moreover, the preparation technology is simple, and once moulding can be realized.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a hydrogel scaffold for bone repair and a preparation method thereof. Background technique [0002] 3D printing hydrogel materials exhibit excellent mechanical properties, drug-loading capacity, and bionic properties. At the same time, because they can be individually polymerized and formed according to the complex structure of the defect site, it is a new type of tissue engineering material that appears late but has great development potential. The material has broad application prospects in clinical practice. At the same time, 3D printing hydrogels also have great potential in the field of biomedical engineering at home and abroad, such as bionic artificial organs, and research on the interaction between stem cells / tumors and materials due to its advanced molding process and damaged tissue repair ability. great research value. [0003] In order to allow cells / tissues to gr...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/22A61L27/20A61L27/12A61L27/38A61L27/50A61L27/52A61L27/56A61L27/58B33Y70/00
CPCA61L27/12A61L27/20A61L27/227A61L27/24A61L27/3834A61L27/50A61L27/52A61L27/56A61L27/58A61L2300/412A61L2400/06A61L2400/12A61L2430/02B33Y70/00C08L89/00C08L5/04C08L5/08
Inventor 杨高洁吴苏州李晓云陈明惠
Owner 深圳市晶莱新材料科技有限公司
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