Myocardial patch and preparation method thereof

A scaffold and hydrogel technology, applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of low efficiency, high cost, immune rejection, etc., and achieve excellent mechanical properties, repair body damage, and excellent effect Effect

Inactive Publication Date: 2016-07-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Some scholars try to implant some decellularized vascular networks on the surface of the cell sheet, or use other methods, such as 3D printing technology, to print the capillary network directly on the surface of the cell sheet. These methods have some shortcomings. , the former is the problem of immune rejection, and the latter has the problems of low efficiency and high cost

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  • Myocardial patch and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 The preparation method of hydrogel scaffold and myocardial patch of the present invention

[0033] 1. Preparation method

[0034] (1) Preparation of hydrogel

[0035] Gelatin (type B kraft gelatin, product of Sigma Company) was weighed quantitatively, placed in PBS, and dissolved in a water bath at 50°C to accelerate the dissolution, so that the gelatin concentration reached 10% (mass volume ratio), and then sterilized by filtration with a 0.2 μm needle filter. Similarly, quantitatively weigh microbial transglutaminase (mTG, the activity is 100U / g, the product of Ajinomoto Co., Ltd., USA), and dissolve it in PBS so that the concentration of mTG reaches 10% (mass volume ratio), and filter after dissolving Sterilized spare. According to the ratio of adding 10 U units of mTG per gram of gelatin, the gelatin aqueous solution (keep the temperature at about 37° C.) and mTG were mixed to obtain a mixed solution.

[0036] (2) Cell preparation

[0037] Get the c...

Embodiment 2

[0044] Embodiment 2 The preparation method of hydrogel scaffold and myocardial patch of the present invention

[0045] 1. Preparation method

[0046] (1) Preparation of hydrogel

[0047] Gelatin (type B kraft gelatin, product of Sigma Company) was weighed quantitatively, placed in PBS, and dissolved in a water bath at 50°C to accelerate the dissolution, so that the gelatin concentration reached 10% (mass volume ratio), and then sterilized by filtration with a 0.2 μm needle filter. Similarly, quantitatively weigh microbial transglutaminase (mTG, the activity is 100U / g, the product of Ajinomoto Co., Ltd., USA), and dissolve it in PBS so that the concentration of mTG reaches 10% (mass volume ratio), and filter after dissolving Sterilized spare. According to the ratio of adding 10 U units of mTG per gram of gelatin, the gelatin aqueous solution (keep the temperature at about 37° C.) and mTG were mixed to obtain a mixed solution.

[0048] (2) Cell preparation

[0049] Get the c...

Embodiment 3

[0056] Embodiment 3 The preparation method of the hydrogel scaffold and myocardial patch of the present invention

[0057] 1. Preparation method

[0058] (1) Preparation of hydrogel

[0059] Gelatin (type B kraft gelatin, product of Sigma Company) was weighed quantitatively, placed in PBS, and dissolved in a water bath at 50°C to accelerate the dissolution, so that the gelatin concentration reached 10% (mass volume ratio), and then sterilized by filtration with a 0.2 μm needle filter. Similarly, quantitatively weigh microbial transglutaminase (mTG, the activity is 100U / g, the product of Ajinomoto Co., Ltd., USA), and dissolve it in PBS so that the concentration of mTG reaches 10% (mass volume ratio), and filter after dissolving Sterilized spare. According to the ratio of adding 10 U units of mTG per gram of gelatin, the gelatin aqueous solution (keep the temperature at about 37° C.) and mTG were mixed to obtain a mixed solution.

[0060] (2) Cell preparation

[0061] Get t...

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Abstract

The invention relates to a myocardial patch and a preparation method thereof and discloses a hydrogel scaffold.The hydrogel scaffold is prepared according to a method including steps: (1) using gelatin for preparing a gelatin solution with the concentration being 4-10%w/v, then preparing a transglutaminase solution, adding the transglutaminase solution into the gelatin solution according to a proportion that per gram of gelatin is added with not less than 5U of transglutaminase, and well mixing to obtain a hydrogel solution; (2) adding cells into the hydrogel solution, well mixing to obtain a hydrogel solution containing the cells, and gelatinizing to obtain the hydrogel scaffold.The hydrogel scaffold is excellent in mechanical property, cell growth normality is guaranteed, and body injuries can be effectively repaired.The myocardial patch prepared from the hydrogel scaffold is 2-4mm in thickness and convenient for surgical suture, and normality in growth of compound cells is ensured.Furthermore, the myocardial patch is excellently effective in myocardia repair and has a promising clinical application prospect.

Description

technical field [0001] The invention relates to a myocardial patch and a preparation method thereof. Background technique [0002] Cardiomyocytes are terminally differentiated cells that are difficult to regenerate after necrosis. The incidence of coronary heart disease is increasing day by day in today's society. Although the current treatment methods can complete the revascularization and avoid further damage to the myocardium, they cannot save the necrotic myocardium. For severe myocardial infarction patients, when the remaining myocardium cannot maintain heart function, heart transplantation has become the only effective treatment. patients cannot receive effective treatment. As a cell therapy method, stem cell implantation provides a very potential solution for the treatment of myocardial infarction. At present, many clinical studies have fully confirmed that stem cell implantation can improve cardiac function in patients with myocardial infarction. However, the big...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/22A61L27/50A61L27/52
CPCA61L27/222A61L27/3826A61L27/383A61L27/3873A61L27/50A61L27/52A61L2430/20C08L89/00
Inventor 杨刚肖正华任小梅钱宏龙海燕郭应强
Owner SICHUAN UNIV
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