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Method of preparing highly branched polysaccharide-fibroin hydrogel bracket

A polysaccharide and hydrogel technology, which is applied in the field of preparation of highly branched polysaccharide-silk fibroin hydrogel scaffolds, can solve the problem of difficult to support cell adhesion and proliferation growth, the large amount of organic raw materials diamine or hydrazine, and the inside of the scaffold and uneven surface crosslinking to achieve good controllable release behavior, improved mechanical properties, and high swelling properties

Inactive Publication Date: 2015-04-29
WUHAN TEXTILE UNIV
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Problems solved by technology

The disadvantage of this method is that the pullulan used is a linear polysaccharide, so in the process of drug controlled release, the drug is released rapidly in the hydrogel, and the amount of organic raw material diamine or hydrazine is large, and there is cytotoxicity, etc. question
The disadvantage of this method is that due to the cross-linking after freeze-forming, the cross-linking agent is difficult to diffuse into the inside of the stent, so that the cross-linking may only occur on the surface of the stent, and the cross-linking inside and on the surface of the stent is not uniform
The common disadvantages of the above methods are: these polysaccharides have a linear chain conformation or a low degree of branching, the strength of the obtained hydrogel scaffold is weak, and the drug is released rapidly in the hydrogel. Therefore, it is suitable for use as biomedical materials or as drug carriers When it is difficult to support cell adhesion and proliferation growth, and the drug release is too fast, the expected effect cannot be achieved

Method used

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  • Method of preparing highly branched polysaccharide-fibroin hydrogel bracket
  • Method of preparing highly branched polysaccharide-fibroin hydrogel bracket
  • Method of preparing highly branched polysaccharide-fibroin hydrogel bracket

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preparation example Construction

[0019] A kind of method for preparing highly branched polysaccharide-silk fibroin hydrogel support, described preparation method comprises the following steps:

[0020] a The dried sclerotia of tiger milk mushroom was crushed, and the fat was removed by Soxhlet extraction with ethyl acetate and acetone for 6 h sequentially. Both ethyl acetate and acetone were chemical pure reagents. Then soak the sclerotium of tiger milk mushroom after fat removal in physiological saline at 80 ℃ for 2 h, centrifuge; extract the residue under high pressure at 120 ℃ for 30 min, and centrifuge at 8000 rpm for 20 min to obtain the extract , after cooling the extract, centrifuge and collect the residue; the residue is centrifugally washed with deionized water and freeze-dried to obtain highly branched tiger milk mushroom polysaccharide, and can also be dried by other methods to obtain highly branched tiger milk mushroom polysaccharide, and the heavy precipitation classification method is used The o...

Embodiment 1

[0028] 0.9 g molecular weight is 4.81´10 6 The hyperbranched polysaccharides of tiger milk mushroom were dispersed in 50 mL of 20 wt% NaOH and isopropanol mixed solution, stirred in an ice-water bath for 2 h to form a uniform suspension, and 7.8 g of chloroacetic acid was dissolved in isopropanol, And slowly added dropwise to the polysaccharide suspension system, and reacted at 60 °C for 3 h. After stopping the reaction, cool to room temperature, then use 0.5 M acetic acid solution to neutralize to pH = 7, dialyze the above product with distilled water, concentrate by rotary evaporation, and freeze-dry to obtain carboxymethylated hyperbranched polysaccharide. The hyperbranched polysaccharides were dissolved in phosphate buffered saline solution with pH=7.4 to obtain a 20% solution, and 100 mg / mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Salt and 150 mg / mL N-hydroxysuccinimide were added to the above solution, stirred for 15 min to obtain an activated syst...

Embodiment 2

[0030] 0.9 g molecular weight is 4.81´10 6 The hyperbranched polysaccharides of tiger milk mushroom were dispersed in 50 mL of 20 wt% NaOH and isopropanol mixed solution, stirred in an ice-water bath for 2 h to form a uniform suspension, and 7.8 g of chloroacetic acid was dissolved in isopropanol, And slowly added dropwise to the polysaccharide suspension system, and reacted at 60 °C for 3 h. After stopping the reaction, cool to room temperature, then use 0.5 M acetic acid solution to neutralize to pH = 7, dialyze the above product with distilled water, concentrate by rotary evaporation, and freeze-dry to obtain carboxymethylated hyperbranched polysaccharide. The hyperbranched polysaccharides were dissolved in phosphate buffered saline solution with pH=7.4 to obtain a 40% solution, and 100 mg / mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Salt and 150 mg / mL N-hydroxysuccinimide were added to the above solution, stirred for 15 min to obtain an activated syst...

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Abstract

The invention discloses a method of preparing a highly branched polysaccharide-fibroin hydrogel bracket and belongs to the technical field of natural polymer materials. The method comprises the following steps: dispersing highly branched polysaccharide of pleurotus tuber-regium into NaOH and isopropanol, carrying out reaction with chloroacetic acid at the temperature of 60 DEG C, carrying out reaction, cooling, neutralization, dialysis and freeze drying to obtain carboxymethyl highly branched polysaccharide, dissolving the obtained carboxymethyl polysaccharide into a phosphoric acid buffer salt solution with a pH value to be 7.4, carrying out activation on 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride and N-hydroxysuccinimide for 15 minutes to 4 hours, and carrying out crosslinking reaction on an activated product and a fibroin solution for 6-48h at the temperature of 4-37 DEG C to obtain the highly branched polysaccharide-fibroin hydrogel bracket. The method is simple in operation and rich in raw material source; in addition, the prepared bracket material has the drug controlled release property and biocompatibility and is good in mechanical property; the highly branched polysaccharide-fibroin hydrogel bracket can be used for preparing an artificial tissue bracket.

Description

technical field [0001] The invention relates to a method for preparing a highly branched polysaccharide-silk fibroin hydrogel support. Belonging to the technical field of natural polymer materials, this highly branched polysaccharide-silk fibroin hydrogel three-dimensional penetrating porous scaffold can be widely used in industries such as tissue engineering materials and controlled release of biomedical drugs. Background technique [0002] Polymer hydrogel has a certain degree of elasticity and a three-dimensional through-porous structure, which is suitable for simulating the extracellular matrix, providing a three-dimensional microenvironment suitable for cell growth and biophysical signals between cells and the extracellular matrix, and maintaining the normal phenotype and physiology of cells. Function. Polysaccharides are a class of degradable natural polymers with abundant sources, and are widely used in tissue engineering and drug controlled release materials. Natur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/52A61L27/56A61K47/42A61K47/36A61K9/06
Inventor 陶咏真张如权徐卫林杨苇柏自奎赵青华
Owner WUHAN TEXTILE UNIV
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