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Preparation method of injectable double-cross-linked hydrogel for tissue engineering

A tissue engineering and injectable technology, applied in the field of preparation of injectable double-cross-linked hydrogel, achieves the effects of good biological inertness, simple operation process and strong mechanical properties

Active Publication Date: 2012-11-21
烟台沃德麦克斯纳米科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Hydrogel is a hydrophilic polymer network, which can absorb a large amount of water, but will not dissolve in water due to the physical cross-linking and chemical cross-linking between polymer chains, and can only swell and maintain a certain shape.

Method used

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  • Preparation method of injectable double-cross-linked hydrogel for tissue engineering
  • Preparation method of injectable double-cross-linked hydrogel for tissue engineering
  • Preparation method of injectable double-cross-linked hydrogel for tissue engineering

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Such as figure 1 As shown in a, add dextran into deionized water, stir magnetically until the dextran is completely dissolved, and make an aqueous solution of dextran with a concentration of 1% by mass. Add sodium periodate (NaIO 4 ), wherein the molar ratio of sodium periodate to the structural unit of dextran was 1:2, and the reaction was stirred in the dark at room temperature (25°C) for 4 hours, then 5ml of ethylene glycol was added, and the stirring was continued for 15 minutes to terminate the reaction. The product was put into a dialysis bag (molecular weight cut-off: 3500) for dialysis for 3 days, and the water was changed 4 times a day. Then the product was taken out and placed in a plastic petri dish, pre-frozen in a low-temperature refrigerator for 12 hours, and the pre-frozen product was freeze-dried to finally obtain a white product of partially aldylated dextran.

Embodiment 2

[0037] Such as figure 1 As shown in b, take gelatin and dissolve it in 0.1M phosphate solution (pH 5.0) to obtain a gelatin aqueous solution with a mass percent concentration of 4%. After it is completely dissolved (the solution is a transparent liquid), add ethylenediamine (ED). Mix evenly with magnetic stirring, adjust to pH=5.0 with 6M HCl, then add carbodiimide (EDC), and react at room temperature for 4 hours; wherein the molar ratio of carboxyl, ethylenediamine and carbodiimide on the gelatin is 1: 40:2. Put the product into a dialysis bag (cut off Mw=3500) and dialyze for 3 days, changing the water 3~4 times a day. After the dialysis is complete, the dialysate is poured into a plastic watch glass, placed in a low-temperature refrigerator for pre-freezing for 24 hours, and the pre-frozen material is freeze-dried to finally obtain a white product of modified gelatin.

Embodiment 3

[0039] Such as figure 1 As shown in c, take 8g of four-walled polyethylene glycol and dissolve it in 200ml of anhydrous dichloromethane, remove a small amount of water in the four-walled polyethylene glycol by azeotropic distillation, cool to room temperature, add triethylamine, and then Acryloyl chloride was added dropwise under the protection of nitrogen, and reacted for 48 hours at room temperature, wherein the molar ratio of four-walled polyethylene glycol, triethylamine and acryloyl chloride was 1:4:8. After that, it was purified by precipitation with excess ether, dissolved again in dichloromethane, precipitated with excess ether, the purification process was repeated three times, and dried under reduced pressure at room temperature to obtain a white product.

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Abstract

The invention relates to a preparation method of injectable double-cross-linked hydrogel for tissue engineering, which comprises the following steps that: an aqueous solution of aminated gelatin, an aqueous solution of aldehyde dextran, and an aqueous solution of terminated vinyl poly ethylene glycol are mixed according to a volume ratio of 4:4:3, then are cross-linked for 10-20 minutes at a temperature of 35 to 40 DED C, and finally are cross-linked for 3 to 8 minutes by ultraviolet irradiation. The preparation method provided by the invention has a simple operation process and mild implementation conditions; and the double-cross-linked hydrogel obtained by the invention is non-toxic and biodegradable, has good mechanical properties and biocompatibility, and can be used for injectable hydrogel scaffold in the tissue engineering.

Description

technical field [0001] The invention belongs to the field of hydrogel preparation, in particular to a preparation method of an injectable double-crosslinked hydrogel which can be used for tissue engineering. Background technique [0002] Hydrogel is a hydrophilic polymer network, which can absorb a large amount of water, but will not dissolve in water due to the physical cross-linking and chemical cross-linking between polymer chains, and can only swell and maintain a certain shape. Hydrogels have good biocompatibility and water permeability, and hydrogel materials with different microstructures and properties can be obtained through artificial synthesis. These properties make hydrogels widely used in the field of biomedicine. [0003] Injectable hydrogel for tissue engineering is to inject cell / carrier complexes into the body to form new tissues, so as to achieve the purpose of repairing body defects. This method has the characteristics of small trauma and simple operation...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/22A61L27/20A61L27/18C08B37/02C08H1/00
Inventor 莫秀梅耿晓华
Owner 烟台沃德麦克斯纳米科技有限公司
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