Mixed hydrogel with characteristics of temperature sensitivity and photosensitivity, and three-dimensional (3D) printing method using mixed hydrogel

A 3D printing and water mixing technology, applied in 3D object support structures, manufacturing tools, additive manufacturing, etc., can solve the problems of poor processability of GelMA hydrogel, hindering GelMA hydrogel printability and 3D printing accuracy , to achieve the effect of improving temperature sensitivity

Active Publication Date: 2018-02-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the processability of low-concentration GelMA hydrogels is poor, which serio

Method used

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  • Mixed hydrogel with characteristics of temperature sensitivity and photosensitivity, and three-dimensional (3D) printing method using mixed hydrogel
  • Mixed hydrogel with characteristics of temperature sensitivity and photosensitivity, and three-dimensional (3D) printing method using mixed hydrogel
  • Mixed hydrogel with characteristics of temperature sensitivity and photosensitivity, and three-dimensional (3D) printing method using mixed hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) Mix methacrylamide-based gelatin (GelMA) and gelatin to prepare an aqueous solution, and add a photoinitiator to obtain a mixed hydrogel solution. The concentration of methacrylamide-based gelatin in the mixed hydrogel solution was 5% (w / v), the concentration of gelatin was 8% (w / v), and the concentration of photoinitiator was 0.5% (w / v). The initiator is LAP;

[0066] (2) Put the mixed hydrogel solution into the syringe of the 3D printer, and control the temperature of the syringe to 20.5°C, so that the mixed hydrogel can be extruded in filaments;

[0067] The print parameters are:

[0068] Extrusion air pressure: 275kPa, XY axis scanning speed: 200mm / min, needle size: 26G (inner diameter 0.26mm);

[0069] Preset grid support size: 9mm×9mm×5mm, line spacing 0.75mm;

[0070] The line diameter of the printed biomimetic hydrogel scaffold is about 0.27mm;

[0071] (3) The hydrogel is extruded onto the temperature-controlled printing platform for further temperature...

Embodiment 2

[0084] (1) Mix methacrylamide-based gelatin (GelMA) and gelatin to prepare an aqueous solution, and add a photoinitiator to obtain a mixed hydrogel solution. The concentration of methacrylamide-based gelatin in the mixed hydrogel solution was 10% (w / v), the concentration of gelatin was 6% (w / v), and the concentration of photoinitiator was 0.5% (w / v). The initiator is LAP;

[0085] (2) Put the mixed hydrogel solution into the syringe of the 3D printer, and control the temperature of the syringe to 21°C, so that the mixed hydrogel can be extruded in filaments;

[0086] The print parameters are:

[0087] Extrusion air pressure: 275kPa, XY axis scanning speed: 200mm / min, needle size: 26G (inner diameter 0.26mm);

[0088] Preset grid support size: 9mm×9mm×5mm, line spacing 0.75mm;

[0089] (3) The hydrogel is extruded onto the temperature-controlled printing platform for further temperature-controlled curing to form a preset grid support structure, and the temperature of the tem...

Embodiment 3

[0092] (1) Mix methacrylamide-based gelatin (GelMA) and gelatin to prepare an aqueous solution, and add a photoinitiator to obtain a mixed hydrogel solution. The concentration of methacrylamide-based gelatin in the mixed hydrogel solution was 15% (w / v), the concentration of gelatin was 4% (w / v), and the concentration of photoinitiator was 0.5% (w / v). The initiator is LAP;

[0093] (2) Put the mixed hydrogel solution into the syringe of the 3D printer, and control the temperature of the syringe to 21.5°C, so that the mixed hydrogel can be extruded in filaments;

[0094] The print parameters are:

[0095] Extrusion air pressure: 275kPa, XY axis scanning speed: 200mm / min, needle size: 26G (inner diameter 0.26mm);

[0096] Preset grid support size: 9mm×9mm×5mm, line spacing 0.75mm;

[0097] (3) The hydrogel is extruded onto the temperature-controlled printing platform for further temperature-controlled curing to form a preset grid support structure, and the temperature of the t...

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Abstract

The invention discloses mixed hydrogel with characteristics of temperature sensitivity and photosensitivity. Every 100ml of the mixed hydrogel is prepared from the following components: 5-30g of methacrylamide-based gelatin, 0.1-10g of gelatin and 0.2-1g of a photoinitiator. The gelatin is added into the mixed hydrogel, so that the printability of low-concentration methacrylamide-based gelatin hydrogel can be improved, and the balance between the physical properties and biological functions of GelMA hydrogel can be well maintained. The invention also discloses a method for curing a 3D printedbiomimetic hydrogel scaffold by two steps of temperature sensitivity and photosensitivity; the method comprises the following steps: (1) putting the mixed hydrogel into a needle cylinder of a 3D printer; (2) extruding the mixed hydrogel in the needle cylinder onto a temperature control printing platform under the condition of preset printing parameters, and controlling the temperature of the temperature control printing platform so as to enable the mixed hydrogel to be preliminarily cured into a preset support structure; (3) carrying out ultraviolet irradiation on the preliminarily cured support structure to enable the support structure to be subjected to ultraviolet crosslinking and curing so as to form a bionic hydrogel support.

Description

technical field [0001] The invention relates to the technical field of biomedical polymer materials, in particular to a hybrid hydrogel with temperature-sensitive and photosensitive properties and a 3D printing method thereof. Background technique [0002] Tissue engineering is an emerging discipline that focuses on the formation and regeneration of tissues and organs. The basic principle and method are to plant the normal tissue cells cultured and expanded in vitro on a tissue engineering porous scaffold with good biocompatibility and which can be gradually degraded and absorbed in vivo to form a cell-scaffold complex. Proliferate and differentiate, and then implant the complex into the lesioned part of the body tissue, continue to proliferate and secrete extracellular matrix in the body, and along with the gradual degradation of the material, form a new tissue or organ that is suitable for its own function and shape, thereby To achieve the purpose of repairing damaged tis...

Claims

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Application Information

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IPC IPC(8): C08L89/06C08F299/00C08F2/48A61L27/22A61L27/52B29C64/106B29C64/264B33Y10/00
CPCA61L27/222A61L27/52B33Y10/00C08F2/48C08F299/00C08L89/06C08L2203/02C08L2205/025
Inventor 尹俊索海瑞严梦玲傅建中
Owner ZHEJIANG UNIV
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