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Method for compounding aquo-gel to porous tissue engineering rack

A porous scaffold and porous tissue technology, which is used in medical science, prosthesis, hybrid cell preparation, etc., can solve the problems of inability to effectively control the shape of regenerated organs, inability to maintain macroscopic shapes, and difficult macroscopic modeling. Matrix structure and biocompatibility, improved biological properties, wide range of effects

Inactive Publication Date: 2005-03-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This type of scaffold also has obvious disadvantages, that is, its mechanical strength is generally poor, it is difficult to shape macroscopically, and it cannot maintain a good macroscopic shape during tissue regeneration, so it cannot effectively control the shape of the regenerated organ; at the same time, its operability is relatively poor

Method used

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  • Method for compounding aquo-gel to porous tissue engineering rack
  • Method for compounding aquo-gel to porous tissue engineering rack
  • Method for compounding aquo-gel to porous tissue engineering rack

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1 Introduction of Polylactic Acid Porous Scaffold into Sodium Alginate Hydrogel Containing Chondrocytes

[0039] Prepare sodium alginate sol with a weight-volume concentration of 2%, put it in an autoclave and sterilize at 120°C for 30 minutes, and after cooling to room temperature, mix sodium alginate and chondrocyte suspension to make the final concentration of sodium alginate 1%. The polylactic acid stent sheet with an average pore size of 300 microns and a thickness of 2-4 mm is soaked in 75% ethanol solution, and the air in the stent is removed by vacuuming, so that the ethanol solution completely infiltrates the stent. After soaking in the ethanol solution for one day, the scaffold was soaked in a phosphate buffer solution with a pH of 7.4 for one day, during which the solution was changed three times to completely replace the ethanol in the scaffold. Add the sol and cell mixture dropwise on the surface of the treated polylactic acid scaffold. Under grav...

Embodiment 2

[0042] Example 2 Introduction of Polylactic Acid Porous Scaffold into Agar Hydrogel Containing Chondrocytes

[0043] Prepare agar sol with a weight-volume concentration of 2%, put it in an autoclave for 120°C high-temperature sterilization for 30 minutes, and after cooling to about 45°C, mix the agar sol with the chondrocyte suspension to make the final agar concentration 1%. The polylactic acid stent sheet with an average pore size of 300 microns and a thickness of 2-4 mm is soaked in 75% ethanol solution, and the air in the stent is removed by vacuuming, so that the ethanol solution completely infiltrates the stent. After soaking in the ethanol solution for one day, the scaffold was soaked in a phosphate buffer solution with a pH of 7.4 for one day, during which the solution was changed three times to completely replace the ethanol in the scaffold. The mixture of sol and cells was dropped on the surface of the treated polylactic acid scaffold. Under gravity and capillary ac...

Embodiment 3

[0045] Example 3 Introduction of Polylactic Acid Porous Scaffold into Chondrocyte-Containing Agar and Gelatin Hydrogel

[0046] Configure agar and gelatin mixed sols with a weight volume concentration of 2%, put them into an autoclave for 120°C high-temperature sterilization for 30 minutes, and after cooling to about 45°C, mix the agar sol, gelatin sol and chondrocyte suspension to make the final The concentrations of agar and gelatin were each 1%. The polylactic acid stent sheet with an average pore size of 300 microns and a thickness of 2-4 mm is soaked in 75% ethanol solution, and the air in the stent is removed by vacuuming, so that the ethanol solution completely infiltrates the stent. After soaking in the ethanol solution for one day, the scaffold was soaked in a phosphate buffer solution with a pH of 7.4 for one day, during which the solution was changed three times to completely replace the ethanol in the scaffold. The mixture of sol and cells was dropped on the surfa...

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Abstract

The present invention discloses the method of compounding aquogel into porous tissue engineering rack. Under sol state, aquogel is first led into porous rack, and the outer condition is altered to convert the sol into gel to obtain porous rack with compounded aquosol. According to requirement, the water sol may have cell growth factor for cell growth and differentiation may be added. The rack the present invention produces possesses the excellent mechanical performance of porous rack and the excellent biocompatibility of aquosol rack simultaneously. The present invention has wide application range, and is suitable for various porous rack with different structures and aquosols and various kinds of cell. The rack may be used widely in tissue engineering field.

Description

technical field [0001] The present invention relates to methods for compounding hydrogels into porous tissue engineering scaffolds. Background technique [0002] It has become a widely recognized method in tissue engineering research to use natural or synthetic materials to simulate the extracellular matrix, make it combine with cells, and then promote and induce cell growth to form tissues or organs, so as to achieve the purpose of repairing or reconstructing human organ defects. The preparation of cell scaffolds and their combination with cells are important issues to be solved in tissue engineering. [0003] According to the morphological structure of tissue regeneration scaffolds, currently widely used scaffolds are divided into two categories: porous scaffolds and hydrogel scaffolds. Porous scaffolds consist of materials and interstices between materials, which are usually air in the dry state and media in a cell culture environment. At present, a variety of methods h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61L27/00C12N15/02
Inventor 高长有龚逸鸿何丽娟马祖伟沈家骢
Owner ZHEJIANG UNIV
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