Method for preparing injectable chitosan hydrogen for tissue engineering

Abstract

The invention discloses a method for preparing an injection chitose aqueous gel used in tissue engineering, grafting the methacrylic acid and lactic acid onto the chitose molecular chain in order with carbodiimide condensation method, the got chitose derivative possesses the polymerized carbon-to-carbon unsaturated bond and water solubility. Employing the redox initiating system of ammonium peroxodisulfate and tetramethylethylenediamine to make the chitose derivative form into chitose aqueous gel through double bond cross-linking. The chitose aqueous gel is non toxic, biodegradable and biological compatibility. The process of the invention is simple and can be used for injection aqueous gel cradle in the tissue engineering.

Description

technical field

[0001] The invention relates to a preparation method of injectable chitosan hydrogel for tissue engineering. Background technique

[0002] Injectable hydrogel scaffold is a fluid hydrogel prepolymer compounded with allogeneic or autologous cells and then directly injected into the defect site of the body, or directly injected into the body without compounding with cells, and the material reaches the defect site A scaffold with certain mechanical strength, shape and exchangeable with body fluids can be formed in situ under physical or chemical stimuli. The scaffold directly injected into the body without compounding with the cells can utilize the cells of the tissues surrounding the injection to expand, grow and proliferate to form tissues. Its advantages mainly lie in the in vivo culture environment of cells and minimal invasiveness. At the same time, hydrogel materials usually have good biocompatibility, and their aqueous environment is conducive to the pr...

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