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Method for preparing injectable chitosan hydrogen for tissue engineering

A tissue engineering and injectable technology, applied in medical science, prosthesis, coating, etc., can solve problems affecting cell activity, histocompatibility, structural instability, etc., and achieve the effect of mild implementation conditions and simple operation process

Inactive Publication Date: 2006-07-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the above chitosan hydrogel is a physical gel, its structure is unstable, and the presence of salt and non-degradable polyisopropylacrylamide will affect the activity of cells and tissue compatibility.

Method used

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  • Method for preparing injectable chitosan hydrogen for tissue engineering
  • Method for preparing injectable chitosan hydrogen for tissue engineering
  • Method for preparing injectable chitosan hydrogen for tissue engineering

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0025] Example 1: Weigh 800 mg of chitosan and place it in a 250 ml Erlenmeyer flask, add 100 ml triple distilled water and 420 μl MA (0.48 mmol), after the CS is completely dissolved, add 930 mg EDAC (0.48 mmol), then stir the reaction at room temperature 24h. In order to remove unreacted MA and other small molecular products, the mixed solution was placed in a dialysis bag with a cut-off molecular weight of 10,000 Da, dialyzed in a large amount of triple-distilled water at room temperature for 3 days, and the triple-distilled water was changed 2 to 3 times a day. Finally, the liquid was frozen and freeze-dried to obtain MA-grafted chitosan (CM). The CM yields are all greater than 90%, and the structural changes are shown in figure 2 ; MA grafting amount is about 23%, see Figure 4 ; Swellable in water. The above 400mg of CM was dissolved in 50ml of three-distilled water containing 210μl of LA (0.2mmol), and after complete dissolution, 460mg of EDAC (0.24mmol) was added. ...

example 2

[0026] Example 2: Weigh chitosan 800mg and place it in a 250ml Erlenmeyer flask, add 100ml three-distilled water and 420μl MA (0.48mmol), after CS is completely dissolved, add 232.5mg EDAC (0.12mmol), then at room temperature, stir Reaction 24h. In order to remove unreacted MA and other small molecular products, the mixed solution was placed in a dialysis bag with a cut-off molecular weight of 10,000 Da, dialyzed in a large amount of triple-distilled water at room temperature for 3 days, and the triple-distilled water was changed 2 to 3 times a day. Finally, the liquid was frozen and freeze-dried to obtain MA-grafted chitosan (CM). The CM yields are all greater than 90%, and the MA grafting amount is about 11.74%, see Figure 4 .

example 3

[0027] Example 3: Dissolve 400mg CM with about 23% MA grafting in 50ml triple-distilled water containing 210μl LA (0.2mmol), and add 115mg EDAC (0.06mmol) after complete dissolution. After the mixture was stirred at room temperature for 24 hours, the mixture was placed in a dialysis bag with a cut-off molecular weight of 10,000 Da, dialyzed in a large amount of triple-distilled water at room temperature for 3 days, and the triple-distilled water was changed 2 to 3 times a day. Finally, the liquid was frozen and freeze-dried to obtain MA and LA grafted chitosan (CML). The yield of CML is greater than 90%, and the amount of LA grafting is about 43.2%, see Figure 5 .

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Abstract

The invention discloses a method for preparing an injection chitose aqueous gel used in tissue engineering, grafting the methacrylic acid and lactic acid onto the chitose molecular chain in order with carbodiimide condensation method, the got chitose derivative possesses the polymerized carbon-to-carbon unsaturated bond and water solubility. Employing the redox initiating system of ammonium peroxodisulfate and tetramethylethylenediamine to make the chitose derivative form into chitose aqueous gel through double bond cross-linking. The chitose aqueous gel is non toxic, biodegradable and biological compatibility. The process of the invention is simple and can be used for injection aqueous gel cradle in the tissue engineering.

Description

technical field [0001] The invention relates to a preparation method of injectable chitosan hydrogel for tissue engineering. Background technique [0002] Injectable hydrogel scaffold is a fluid hydrogel prepolymer compounded with allogeneic or autologous cells and then directly injected into the defect site of the body, or directly injected into the body without compounding with cells, and the material reaches the defect site A scaffold with certain mechanical strength, shape and exchangeable with body fluids can be formed in situ under physical or chemical stimuli. The scaffold directly injected into the body without compounding with the cells can utilize the cells of the tissues surrounding the injection to expand, grow and proliferate to form tissues. Its advantages mainly lie in the in vivo culture environment of cells and minimal invasiveness. At the same time, hydrogel materials usually have good biocompatibility, and their aqueous environment is conducive to the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/52
Inventor 高长有洪奕沈家骢
Owner ZHEJIANG UNIV
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