Intelligent hydroxybutyl chitosan hydrogel stent based on 3D printing technology and preparation method thereof

A technology for printing hydroxybutyl chitosan water and hydroxybutyl chitosan, which is used in medical science, prosthesis, additive processing, etc.

Inactive Publication Date: 2017-04-05
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Intelligent hydroxybutyl chitosan hydrogel stent based on 3D printing technology and preparation method thereof
  • Intelligent hydroxybutyl chitosan hydrogel stent based on 3D printing technology and preparation method thereof
  • Intelligent hydroxybutyl chitosan hydrogel stent based on 3D printing technology and preparation method thereof

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Embodiment 1

[0022] (1) Take hydroxybutyl chitosan with a hydroxybutyl substitution degree of 1.8, dissolve it in pure water to form a sol of 8.0 mg / mL, load it in a 3D printing cylinder, and let it stand at 4°C for defoaming, and then Place it in a water bath at 30°C and raise the temperature for 15 minutes to make it undergo a gelation transition to obtain a hydrogel that can be used for 3D printing.

[0023] (2) Use the 3D bioprinter to adjust the 3D printing parameters, which are: the temperature of the barrel is 30°C, the inner diameter of the needle is 0.20mm, the printing speed is 5mm / s, the distance between the XY axes is 2, 3, 4mm, and the lines between layers The rotation angle is 90°, the Z-axis step height is 0.2mm, the Z-axis layer height is 25 layers, the extrusion pressure is 2.5Bar, and the printing platform temperature is 35°C. Finally, a preliminarily formed grid-like 3D printed hydroxybutyl chitosan hydrogel scaffold was obtained.

[0024] (3) Soak the preliminarily for...

Embodiment 2

[0027] (1) Take hydroxybutyl chitosan with a hydroxybutyl substitution degree of 1.1, dissolve it in pure water to form a sol of 10.0 mg / mL, load it in a 3D printing cylinder, and let it stand at 4°C for defoaming, and then Place it in a water bath at 28°C and raise the temperature for 20 minutes to cause a gelation transition to obtain a hydrogel that can be used for 3D printing.

[0028] (2) Use the 3D bioprinter to adjust the 3D printing parameters, which are: the temperature of the barrel is 28°C, the inner diameter of the needle is 0.20mm, the printing speed is 8mm / s, the distance between the XY axes is 1.0mm, and the step height of the Z axis is 0.3 mm, the extrusion pressure is 2.1Bar, and the temperature of the printing platform is 35°C. The interlayer angle of the lines from the first layer to the third layer is 90°, and the interlayer angle of the lines from the third layer to the fifth layer is 0°. Finally, a preliminary formed 3D printed hydroxybutyl chitosan hydr...

Embodiment 3

[0032] (1) Take hydroxybutyl chitosan with a hydroxybutyl substitution degree of 2.8, dissolve it in pure water to form a sol of 9.0 mg / mL, load it in a 3D printing cylinder, and let it stand at 3°C ​​for defoaming, and then Place it in a water bath at 25°C and raise the temperature for 20 minutes to make it undergo a gelation transition to obtain a hydrogel that can be used for 3D printing.

[0033] (2) Use the 3D bioprinter to adjust the 3D printing parameters, which are: the temperature of the barrel is 25°C, the inner diameter of the needle is 0.13mm, the printing speed is 3mm / s, the distance between the XY axes is 1.0mm, and the step height of the Z axis is 0.2 mm, the extrusion pressure is 3.1Bar, and the temperature of the printing platform is 30°C. The interlayer angle of the lines from the first layer to the third layer is 90°, and the interlayer angle of the lines from the third layer to the fifth layer is 0°. Finally, a preliminary formed 3D printed hydroxybutyl ch...

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Abstract

The invention discloses an intelligent hydroxybutyl chitosan hydrogel stent based on the 3D printing technology and a preparation method of the intelligent hydroxybutyl chitosan hydrogel stent based on the 3D printing technology. The intelligent hydrogel, thermo-sensitive hydroxybutyl chitosan, which is of a single system and has high responsiveness to temperature and ion strength is adopted, the feature that sol-gel phase change is caused by the temperature and the ion strength of the thermo-sensitive hydroxybutyl chitosan is utilized, and the formed hydrogel stent is obtained through adjustment and control over the temperature and 3D printing parameters; then, through inorganic-salt-induced phase change, the hydrogel stent is evenly shrunk, and the mechanical strength and the precision of the hydrogel stent are further improved. The intelligent hydroxybutyl chitosan hydrogel stent based on the 3D printing technology overcomes the defects that 3D printing hydrogel is generally poor in formability, low in mechanical strength, low in precision and the like. Compared with existing common methods of adopting photosensitizer, complex-system hydrogel and the like, the intelligent hydroxybutyl chitosan hydrogel stent based on the 3D printing technology does not need introducing of any photosensitizer or cross-linking agent, is simple in system and gentle in technology; and the prepared 3D stent has high mechanical strength and is provided with a precise internal structure, and the prepared 3D stent can be widely applied to tissue engineering and tissue repair of the cartilage, the skin, the vessel, the nerve, the myocardium and the like.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a hydroxybutyl chitosan intelligent hydrogel scaffold based on 3D printing technology and a preparation method thereof, which can be applied to tissue engineering and tissue repair. Background technique [0002] In recent years, the application of 3D printing technology in the fields of biomedicine and regenerative medicine has attracted extensive attention from researchers. 3D printed tissue engineering scaffolds have unique advantages in accuracy, pore adjustment, internal structure, and personalization, and are developing rapidly in the fields of bone tissue regeneration, drug sustained release, soft tissue repair, and conceptual models. The materials commonly used in 3D printing mainly include metal materials, bioceramics, synthetic polymers and natural degradable polymer hydrogels. Among them, natural degradable polymer hydrogel has an extracellular matrix-lik...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/50A61L27/52A61L27/56A61L27/60B33Y10/00B33Y70/00B33Y80/00
CPCA61L27/20A61L27/50A61L27/507A61L27/52A61L27/56A61L27/60A61L2430/06A61L2430/20A61L2430/32B33Y10/00B33Y70/00B33Y80/00C08L5/08
Inventor 魏长征王晓彤蒋丽霞
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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