Double-sustained-release drug-loaded hydrogel dressing with semi-interpenetrating network entrapped double-layer microspheres as well as preparation method and application thereof

A semi-interpenetrating network and microsphere technology, which is applied in the field of double slow-release drug-loaded hydrogel dressings and its preparation, can solve the problems of inhomogeneous structure and inability to accurately release drugs, achieve good biocompatibility, and improve encapsulation rate, the effect of increasing the drug loading rate

Active Publication Date: 2021-11-16
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hydrogel drug-loaded dressings are degradable and have good biocompatibility, which can effectively solve the above problems. However, traditional hydrogel drug-loaded dressings have defects such as uneven structure and inability to accurately release drugs.

Method used

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  • Double-sustained-release drug-loaded hydrogel dressing with semi-interpenetrating network entrapped double-layer microspheres as well as preparation method and application thereof
  • Double-sustained-release drug-loaded hydrogel dressing with semi-interpenetrating network entrapped double-layer microspheres as well as preparation method and application thereof
  • Double-sustained-release drug-loaded hydrogel dressing with semi-interpenetrating network entrapped double-layer microspheres as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Orthogonal experiment is carried out the preparation of double-layer microspheres, relevant factors and corresponding levels are as follows:

[0030] Firstly, four sets of orthogonal experiments were carried out, and the morphology of the microspheres produced by the four sets of experiments was compared to determine the best formulation for the preparation of calcium alginate microspheres loaded with bovine serum albumin.

[0031] a. Measure 2mL of sodium alginate solution with a mass concentration of 4%, and load bovine serum albumin with the mass volume ratio of bovine serum albumin and sodium alginate solution at 12.5g / L for the experiment.

[0032] b. Measure 2 mL of sodium alginate solution with a mass concentration of 4%, and load bovine serum albumin with the mass volume ratio of bovine serum albumin to sodium alginate solution at 25 g / L for the experiment.

[0033] c. Measure 2mL of sodium alginate solution with a mass concentration of 2%, and loa...

Embodiment 2

[0050] Embodiment 2: Orthogonal test for the preparation of drug-loaded hydrogel, the relevant factors and corresponding levels are as follows:

[0051] Firstly, six sets of orthogonal experiments were carried out to change the content of cross-linking agent potassium persulfate and sodium thiosulfate, and the size and electron microscope morphology of the gel obtained by the six sets of experiments were compared to determine the best formula for hydrogel preparation.

[0052] a. Select 0.1 g of chitosan with a molecular weight of 500,000, 10 mL of glacial acetic acid, 0.4 mL of acrylic acid, 0.3396 mL of 2-ethyl methacrylate, and 0.095 mL of oligo(ethylene glycol) methyl ether methacrylate, 0.02 g potassium persulfate and 0.02 g sodium thiosulfate were tested;

[0053] b. Select 0.1 g of chitosan with a molecular weight of 500,000, 10 mL of glacial acetic acid, 0.4 mL of acrylic acid, 0.3396 mL of 2-ethyl methacrylate, and 0.095 mL of oligo(ethylene glycol) methyl ether metha...

Embodiment 3

[0064] Embodiment 3: the preparation of the hydrogel loaded with double-layer microspheres, the specific experimental process is as follows:

[0065] Put 0.1 g chitosan (molecular weight: 500,000, degree of deacetylation: 95%) in a beaker, take 10 mL of 1% acetic acid aqueous solution, stir until completely dissolved; weigh 0.4 mL of acrylic acid, 0.3396 mL of methacrylic acid 2-Ethyl ester and 0.095mL oligo(ethylene glycol) methyl ether methacrylate monomer were added to the chitosan solution, and stirred thoroughly for 30 min to make it evenly mixed.

[0066] The mixture of 0.03 g potassium persulfate and 0.03 g sodium thiosulfate was dissolved in 0.2 mL of aqueous solution, and the mixed solution was added into the mixed solution of chitosan and monomer, stirred for 20 min to fully dissolve, and 0.2 ~0.4 mL gentamicin sulfate and add 0.02~0.05 g double-layer microspheres.

[0067] The mixed solution was poured into the mold, nitrogen gas was continuously passed for 15 minu...

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Abstract

The invention belongs to the technical field of medical dressings for repairing burn skin wounds, and provides a double-sustained-release drug-loaded hydrogel dressing with double-layer microspheres entrapped by a semi-interpenetrating network. Acrylic acid is used as a pH-sensitive monomer. The pH / temperature dual-sensitive intelligent hydrogel takes methacrylic acid 2-ethyl ester and oligomeric (ethylene glycol) methyl ether methacrylate as temperature-sensitive monomers, and an anti-inflammatory drug gentamicin sulfate is entrapped; the preparation method comprises the following steps: preparing a calcium alginate core sphere entrapped with bovine serum albumin through a high-voltage electrostatic instillation method, coating the surface of the core sphere with a chitosan shell layer entrapped with an antibacterial drug azithromycin to form a double-layer microsphere, and loading the double-layer microsphere into the hydrogel to obtain the hydrogel dressing capable of precisely and slowly releasing two drugs and one protein. According to the pH / temperature change of a wound surface microenvironment, the hydrogel can achieve accurate controlled release of drugs and improve the utilization rate of the drugs, and is a novel double-sustained-release drug-loaded hydrogel dressing with a semi-interpenetrating network combined double-layer microsphere structure and good comprehensive performance.

Description

technical field [0001] The invention belongs to the technical field of medical dressings for repairing burned skin wounds, and in particular relates to a double-sustained-release drug-loaded hydrogel dressing containing double-layer microspheres in a semi-interpenetrating network and a preparation method and application thereof. Background technique [0002] Skin damage caused by burns can easily lead to bacterial infection, which can threaten the life and health of patients in severe cases. As a covering for wounds, medical dressings can replace damaged skin as a temporary barrier during the wound healing process, providing an environment conducive to wound healing. At present, most medical dressings are foam dressings. If there is a large amount of exudate in the wound, the foam that absorbs a large amount of exudate will seep back to the wound surface, and the wound surface will be prone to whitishness and ulceration after being soaked in the exudate for a long time. Nor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00C08F220/06C08F220/34C08F220/28
CPCA61L26/008A61L26/0014A61L26/0066A61L26/0023A61L26/0047A61L26/009C08F220/06A61L2300/41A61L2300/232A61L2300/622C08F220/34C08F220/286Y02A50/30
Inventor 刘阳安美文王馨悦所楠楠丁婧颜晓蝶张文慧郭继强杜苗苗
Owner TAIYUAN UNIV OF TECH
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