Method for preparing polyving akohol/nanosized silica composite hydrogel scaffold through 3D printing

A technology of nano-silica and composite hydrogel, applied in medical science, prosthesis, etc., can solve the problems of low strength, insufficient fineness of the scaffold, and the inability to realize the controllable and adjustable scaffold structure and holes, and achieve internal Effect of controllable porosity and good softness

Inactive Publication Date: 2015-06-24
HUBEI UNIV OF TECH
View PDF3 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some shortcomings in the preparation of PVA hydrogel bioscaffolds - the shape of the scaffold and the internal pores of the scaffold cannot be adjusted conveniently, and the fineness of the scaffold is not high enough.
And PVA hydrogel is applied to articular cartilage replacement material, its strength is still low
[0003] Chinese Patent Publication No. CN101544767A discloses a method for preparing a biocompatible high-strength three-dimensional interconnected porous PVA hydrogel. The method uses polyvinyl alcohol as a matrix material, utilizes surfactants and soluble solid particles to form pores, and uses Three-dimensional porous polyvinyl alcohol hydrogel was prepare

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing polyving akohol/nanosized silica composite hydrogel scaffold through 3D printing
  • Method for preparing polyving akohol/nanosized silica composite hydrogel scaffold through 3D printing
  • Method for preparing polyving akohol/nanosized silica composite hydrogel scaffold through 3D printing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Step 1: Preparation of materials, including the following sub-steps:

[0033] Sub-step 1.1: Add 8g of PVA resin to 92g of deionized water, stir and disperse evenly, and then place it in a constant temperature water bath at 95°C to condense and reflux for 2 hours until the PVA is completely dissolved to obtain 100g of PVA solution;

[0034] Sub-step 1.2: Place 100g of the PVA solution prepared in sub-step 1.1 in a vacuum oven at 95°C for degassing for 30 minutes. The fumed nano-silicon oxide (SiO 2 ) in a beaker, fully stirred until the gas-phase nano-silica is uniformly dispersed in the PVA solution to obtain a mixed sol with thixotropic properties;

[0035] Step 2: Forming of the Bracket:

[0036] The model used is The Loctite ?200D Benchtop Robot's robotic dispensing machine drives a medical syringe connected to a pipette dripper with a diameter of 0.1mm to extrude the mixed sol prepared in step 1, adjust the XY axis platform movement speed to 100mm / min, and the Z ...

Embodiment 2

[0042] Step 1: Preparation of materials, including the following sub-steps:

[0043] Sub-step 1.1: Add 10g of PVA resin to 90g of deionized water, stir and disperse evenly, and then place it in a constant temperature water bath at 90°C for 3 hours to condense and reflux until the PVA is completely dissolved to obtain a PVA solution;

[0044] Sub-step 1.2: Place the PVA solution prepared in sub-step 1.1 in a 95°C vacuum oven for degassing for 30 minutes. Fumed silicon oxide (SiO 2 ) in a beaker, fully stirred until the nano-silica is uniformly dispersed in the PVA solution to obtain a mixed sol with thixotropic properties;

[0045] Step 2: Forming of the Bracket:

[0046] Use the robot dispensing machine model Sistema Dosificador Ultra 2800 to drive a medical syringe connected to a pipette dropper with a diameter of 0.5mm to extrude the mixed sol prepared in step 1, and adjust the moving speed of the XY axis platform to 220mm / min, Z Shaft stepping height 0.5mm, extruder extr...

Embodiment 3

[0053] Step 1: Preparation of materials, including the following sub-steps:

[0054] Sub-step 1.1: Add 12g of PVA resin into 88g of deionized water, stir and disperse evenly, and then condense and reflux in a constant temperature water bath at 95°C for 2 hours until the PVA is completely dissolved to obtain 100g of PVA solution;

[0055] Sub-step 1.2: Place 100g of the PVA solution prepared in sub-step 1.1 in a vacuum oven at 90°C for degassing for 60 minutes. After degassing, slowly add the degassed PVA solution into the gas phase containing 12g Silicon oxide (SiO 2 ) in a beaker, fully stirred until the nano-silica is uniformly dispersed in the PVA solution to obtain a mixed sol with thixotropic properties;

[0056] Step 2: Forming of the Bracket:

[0057] The model used is The Loctite ? The robot dispensing machine of 400D Benchtop Robot drives the medical syringe with a fine needle tube with a diameter of 0.3mm to extrude the mixed sol prepared in step 1, adjust the mo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Apparent viscosityaaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for preparing a polyving akohol/nanosized silica composite hydrogel scaffold through 3D printing. The method comprises the steps that firstly, polyving akohol (PVA) resin with the mass concentration of 8-12% is added into deionized water to be stirred and dispersed, and is completely decomposed in a thermostatic waterbath of 90 DEG C to 95 DEG C to obtain a PVA solution; the obtained PVA solution is slowly added into inorganic nano silicon oxide (SiO2), and is evenly stirred to obtain mixed collosol with the thixotropic property, wherein the mass ratio of the nano SiO2 to the PVA is (0.5-1):1; then, a robot dispenser is utilized for extruding the mixing collosol, and through 3D printing forming, a collosol scaffold sample is obtained; and finally, the sample is placed in a freezing chamber at the temperature of minus 20 DEG C to minus 40 DEG C to be frozen for 20 hours to 22 hours, after the sample is taken out of the chamber, the sample is unfrozen and melted for 2 hours to 4 hours at the room temperature, and the hydrogel scaffold of a controllable fine structure is obtained. The method can conveniently and rapidly prepare the biological scaffold, and controllability of a support structure and inner pores is achieved, so that the method adapts to needs of different application occasions.

Description

technical field [0001] The present invention relates to a method for rapid prototyping of hydrogel biological scaffolds, more specifically, the present invention relates to a method for preparing polyvinyl alcohol / nano-silica composite hydrogel scaffolds by 3D printing, which belongs to biomedical polymer materials technology field. Background technique [0002] Human beings often cause damage to articular cartilage due to aging, accidents and other reasons, and cartilage, as a tissue with very weak regenerative ability, is a major problem in its repair. At present, the most clinically used method is to repair with biological materials, but this method will cause the body's rejection reaction. With the development of tissue engineering technology, using biological scaffolds as carriers, combined with cartilage seed cells and growth factors, to construct cartilage tissue through in vivo or in vitro culture to repair injured tissue is a very promising repair method. The biol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/52A61L27/02A61L27/16
Inventor 李学锋李坚闫晗杨倩龙世军
Owner HUBEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap