A method of assessing microplastic reproductive toxicity

By using the FXR1 protein phase separation reporter system and fluorescence microscopy, the problem of the inability to rapidly and mechanistically assess the reproductive toxicity of microplastics in existing technologies has been solved, enabling rapid, visualized, and high-throughput assessment of reproductive toxicity, which is suitable for large-scale screening.

CN122631602APending Publication Date: 2026-08-25HAINAN UNIV
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Patent Information

Application Number
CN202610672623.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-15
Publication Date
2026-08-25

AI Technical Summary

Technical Problem

Existing technologies lack rapid, mechanism-related, visualized, and high-throughput methods for assessing the reproductive toxicity of microplastics, especially failing to reflect the reproductive toxicity effects of early molecular events.

Method used

The FXR1 protein phase separation reporter system was used to observe the effect of microplastics on the phase separation phenomenon of FXR1 protein through in vitro phase separation system or intracellular reporter system using fluorescence microscopy imaging technology, and the reproductive toxicity risk was quantitatively assessed by fluorescence bleaching recovery technology.

Benefits of technology

It enables rapid assessment of microplastic reproductive toxicity within 24 hours, reflects the reproductive toxicity mechanism in a mechanism-related manner, visualizes and detects droplet morphology changes, and quantifies the reproductive toxicity risk, thus avoiding the ethical issues of animal experiments.

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Abstract

The application discloses a method for evaluating the reproductive toxicity of microplastics, comprising the following steps: (1) providing a FXR1 protein phase separation report system, which is selected from an in vitro phase separation system or an intracellular report system; (2) contacting a microplastic sample to be tested with the FXR1 protein phase separation report system; (3) observing and detecting the influence of the microplastic sample to be tested on the FXR1 protein phase separation phenomenon in the report system by using fluorescence microscopic imaging technology, wherein the influence includes the morphological change of FXR1 protein phase separation droplets and / or the flowability change of FXR1 protein phase separation droplets; (4) evaluating the reproductive toxicity risk level of the microplastic sample to be tested based on the detection result of step (3).
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