Corneal carrier material and preparation method thereof

A stent material and cornea technology, applied in the preparation of corneal stent materials and the field of corneal stent materials, can solve the problems of different ethical issues of corneal curvature of rejection reaction, fast degradation, poor mechanical properties, etc., so as to reduce the probability of rejection reaction, The effect of rational utilization of medical resources and improving the success rate of transplantation

Active Publication Date: 2019-04-05
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Tissue-engineered corneal carrier scaffolds made of natural biomacromolecules such as collagen, gelatin, and silk fibroin still have defects such as insufficient structural density, poor mechanical properties, and rapid degradation; tissue-engineered corneas made of synthetic materials Carrier scaffolds have the disadvantages of not ideal biocompatibility and high immunogenicity
In recent years, tissue-engineered corneal carrier scaffolds made of natural biomaterials such as xenogeneic (pig and other animal sources) corneal stroma that are closest to the natural state have become research hotspots, but their potential rejection, different corneal curvatures and Ethical issues make it difficult to become the best carrier material accepted by Chinese people forever

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  • Corneal carrier material and preparation method thereof
  • Corneal carrier material and preparation method thereof

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preparation example Construction

[0050] The invention provides a preparation method of corneal stent material, comprising:

[0051] The homogenate solution of human corneal stroma is decellularized and shaped to obtain a corneal stent material;

[0052] Wherein, the human corneal stroma is the remaining tissue of the donor cornea after penetrating keratoplasty, the remaining tissue of the donor cornea after lamellar keratoplasty, or the corneal stroma lens after femtosecond laser surgery.

[0053]The preparation method of the corneal stent material provided by the invention has the advantages of simple and convenient process, reasonable use of medical resources, and is suitable for large-scale production and clinical popularization and application. This method uses heterogeneous corneal stroma homogenate as raw material to reconstruct the artificial corneal scaffold material on the basis of retaining the components of the natural extracellular matrix of the cornea. On the one hand, the prepared corneal scaffo...

Embodiment 1

[0110] The present embodiment provides a kind of corneal stent material, and its preparation method comprises the steps:

[0111] (a) Under the dissecting microscope in the biosafety cabinet, remove the sclera and conjunctiva around the cornea from the remaining tissue of the donor cornea after penetrating keratoplasty, wash it three times with sterile PBS solution (1×), and then remove the sclera and conjunctiva around the cornea. The processed corneal tissue was stored in an ultra-low temperature refrigerator.

[0112] (b) Take it out and rinse with sterile PBS solution (1×) for rewarming, then place it in a low-temperature environment of -180°C, and rewarm to 20°C after 75s, repeat 3 times, and then rinse with sterile PBS repeatedly.

[0113] (c) After removing the surface moisture, put it into a grinding container and place it in a low temperature environment of -180°C, run it with a grinder for 20s-20s pre-cooling, break it into powder after three cycles, add ultrapure wa...

Embodiment 2

[0122] The present embodiment provides a kind of corneal stent material, and its preparation method comprises the steps:

[0123] (a) Under the dissecting microscope in the biosafety cabinet, remove the sclera and conjunctiva around the cornea from the remaining tissue of the donor cornea after lamellar keratoplasty, wash it three times with sterile PBS solution (1×), and then remove the sclera and conjunctiva around the cornea. The processed corneal tissue was stored in an ultra-low temperature refrigerator.

[0124] (b) Take it out and rinse with sterile PBS solution (1×) for rewarming, then place it in a low-temperature environment of -200°C, rewarm to 25°C after 45s, repeat this process 3 times, and then rinse it with sterile PBS repeatedly.

[0125] (c) After removing the surface moisture, put it into a grinding container and place it in a low temperature environment of -200°C, run it with a grinder for 40s--40s pre-cooling, break it into powder after three cycles, add ul...

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Abstract

The invention provides a corneal carrier material and a preparation method thereof and relates to the technical field of tissue engineering. The preparation method of the corneal carrier material, provided by the invention, comprises the steps of subjecting repeated-frozen-thawed human corneal stroma homogenate to decellularizing and then carrying out treatment molding. According to the method, the process is simple and convenient, medical resources are utilized reasonably, and thus, the method is applicable to large-scale production and clinical popularization and application. According to the method, homogeneous corneal stroma homogenate is used as a raw material, and an artificial corneal carrier material is reconstructed on the basis of reserving natural extracellular matrix componentsof cornea, so that the prepared corneal support material has good optical properties, a three-dimensional structure and biocompatibility. Through removing remaining antigenic components from cornea by decellularizing, the probability of an exclusive reaction after corneal transplantation can be lowered, and the success rate of transplantation is increased.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a preparation method of a corneal stent material and the corneal stent material. Background technique [0002] The cornea is a layer of transparent film located at the front of the eyeball, covering the iris, pupil and anterior chamber, and provides most of the refractive power for the eye. Because the cornea is in direct contact with the external environment, it is extremely vulnerable to mechanical trauma, thermal burns, and microbial infections, which can cause corneal lesions. In mild cases, the transparency of the cornea will decrease and affect the patient's vision. In severe cases, it will cause corneal opacity and blindness. According to WHO statistics, there are currently nearly 60 million blind patients with corneal disease in the world, and nearly 5 million people in my country. The vast majority of patients can be cured by corneal transplantation, but due t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3604A61L27/3687A61L27/3691A61L2430/16
Inventor 黄挺
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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