Preparation of polyglycolic acid-porcine islet complex

A technology of polyglycolic acid and complexes, which is applied in the field of preparation of porcine islet transplantation complexes, can solve the problems of difficult survival and in vitro culture of porcine islet cells, and achieve the goal of improving the survival time in vitro, increasing the number, and improving the success rate Effect

Inactive Publication Date: 2009-05-20
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that there is a difference between the in vitro culture environment of pig islets and the environment in vivo, which makes it difficult for pig islet cells to survive in vitro and difficult to cultivate in vitro, and to provide a polyglycolic acid-porcine islet complex Preparation

Method used

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  • Preparation of polyglycolic acid-porcine islet complex
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  • Preparation of polyglycolic acid-porcine islet complex

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specific Embodiment approach 1

[0012] Specific embodiment 1: The polyglycolic acid-porcine islet complex of this embodiment is prepared according to the following steps: 1. Treat the polyglycolic acid scaffold, and embed the polyglycolic acid scaffold with a polylysine solution with a concentration of 10 mg / mL for 30 min , and then put it into a clean bench to dry it, then soak it in an ethanol solution with a volume concentration of 75% for 30 minutes, then wash it with PBS buffer solution for 3 to 4 times, then put it into a clean bench to dry it, and irradiate it with a UV lamp 1h; 2. Three-dimensional microgravity cell culture, put the polyglycolic acid scaffold into the tissue culture container of the three-dimensional microgravity tissue cell culture system, add 1mL medium, and then place the porcine islet cells evenly on the polyglycolic acid scaffold, Then add RPMI-1640 culture medium, then empty the air bubbles in the tissue culture container, cover the tissue culture container tightly, and put the ...

specific Embodiment approach 2

[0016] Specific embodiment two: The difference between this embodiment and specific embodiment one is: the relative molecular mass of poly-lysine in step one poly-lysine solution is 1.5×10 4 ~3×10 4 . Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0017] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the relative molecular mass of polylysine in step one polylysine solution is 2×10 4 ~2.5×10 4 . Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a preparation method of a polyglycolic acid and pig islet compound, relates to a preparation method of a pig islet transplanting compound, and solves the problems that as current in vitro cultivating environment of pig islet is different from in vivo environment, pig islet cells have difficult in vitro survival and uneasy in vitro cultivation. The preparation method of the polyglycolic acid and pig islet compound comprises the following steps: 1. polyglycolic acid stent is processed; and 2. three-dimensional micro-gravity cells are cultivated. The polyglycolic acid and pig islet compound that is prepared by the preparation method raises the in vitro cultivating quality and in vitro survival time of pig islet cells, can improve the activity and insulin secreting function of pig islet cells, increases the number of pig islet cells with activity and insulin secreting function, and then achieves the goal for raising islet transplanting success ratio and provides physical foundation for curing diabetes mellitus in future.

Description

technical field [0001] The invention relates to a preparation method of a porcine islet transplantation compound. Background technique [0002] The pig insulin molecule is the closest to the human insulin molecule, with only one amino acid difference. Porcine insulin is similar to human insulin in its blood sugar setting point. Porcine islet cells can survive and proliferate in human serum. Clinical experiments have proved that porcine islets can effectively and safely treat human type 1 diabetes. [0003] Pig islet tissue comes from a wide range of sources, and it is the main source of islet cell transplantation for human type 1 diabetes and some type 2 diabetes. [0004] The key to the success of islet transplantation is whether the patient has obtained a considerable number of active and functional porcine islet cells. [0005] In vitro culture before transplantation is the main method to preserve porcine islet grafts. This method can not only reduce the immunogenicity ...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/071
Inventor 宋纯黄玉东姜再兴谷红波
Owner HARBIN INST OF TECH
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