Fresh keeping agent and application thereof in transportation and fresh keeping of tissue culture seedlings out of culture medium
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A technology of preservatives and culture medium, applied in the field of preservatives, can solve the problems of increasing transportation costs, infectious bacteria, pollution loss and the like
Pending Publication Date: 2021-04-30
漳州悦阳生物科技有限公司
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[0003] When the current tissue culture seedlings are shipped and transported, if there is a culture medium, there will be a real problem of heavy transportation, increased transportation costs and easy to be infected with bacteria on the way, resulting in pollution and loss
If the medium is removed for transportation, the weight can be greatly reduced, the transportation cost can also be reduced, an
Method used
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Embodiment 1
[0030] In this embodiment, a preservative is made of a culture medium as a base, and white sugar and an active retaining agent are added to the base and mixed.
[0031] Wherein, the medium is MS medium, and the mass concentration of the sugar in the raw material is 20g / L; the activity maintaining agent is plant cytokinin 6-BA, and the mass concentration in the raw material is 5ppm .
[0032] In addition, the medium in this example is a freshly prepared MS medium, which is prepared by mixing components with the following mass concentrations:
[0033]
Embodiment 2
[0035] In this embodiment, a preservative is made of a culture medium as a base, and white sugar and an active retaining agent are added to the base and mixed.
[0036] Wherein, the medium is MS medium, and the mass concentration of the sugar in the raw material is 20g / L; when the activity maintaining agent is KT kinetin, its mass concentration in the raw material is 5ppm.
[0037] In addition, the medium in this example is a freshly prepared MS medium, which is prepared by mixing components with the following mass concentrations:
[0038]
Embodiment 3
[0040] In this embodiment, a preservative is made of a culture medium as a base, and white sugar and an active retaining agent are added to the base and mixed.
[0041] Wherein, the medium is MS medium, and the mass concentration of the sugar in the raw material is 20g / L; the activity maintaining agent is CPPU, and the mass concentration in the raw material is 0.6ppm.
[0042] In addition, the medium in this example is a freshly prepared MS medium, which is prepared by mixing components with the following mass concentrations:
[0043]
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Abstract
The invention discloses a fresh-keeping agent and application thereof in transportation and fresh keeping of tissue culture seedlings out of a culture medium. The fresh-keeping agent is prepared by taking the culture medium as a substrate and adding white sugar and an activity keeping agent into the substrate and mixing the raw materials, the fresh-keeping agent can be applied to the tissue culture seedlings out of the culture medium, nutrients required by plants and components for keeping plant activity are added and sprayed on seedlings out of the culture medium in a liquid fertilizer manner, so that on one hand, the nutrients required in the transportation process of the tissue culture seedlings are provided, on the other hand, the tissue culture seedlings can keep activity in the transportation process, meanwhile, the conditions of dead leaves, yellow leaves and weak growing power are greatly reduced, and the survival rate of the tissue culture seedlings is increased; and in addition, according to the scheme, the application method of the fresh-keeping agent is simple, operation is convenient, and the fresh-keeping agent can be suitable for industrial implementation.
Description
technical field [0001] The invention relates to the technical field of antistaling agents, in particular to an antistaling agent and its application in transportation and preservation of tissue cultured seedlings from culture medium. Background technique [0002] Plant tissue culture, that is, plant aseptic culture technology, is based on the theory that plant cells are totipotent, using isolated plant organs such as roots, stems, leaves, stem tips, flowers, fruits, etc.) tissues (such as cambium, epidermis, Cortex, medullary cells, endosperm, etc.) or cells (such as megaspores, microspores, somatic cells, etc.) and protoplasts can induce callus under sterile and suitable artificial medium and artificial conditions such as light and temperature Tissues, adventitious buds, adventitious roots, and finally the discipline of forming a complete plant. [0003] When the current tissue culture seedlings are shipped and transported, if the culture medium is carried, there will be a...
Claims
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