Fresh keeping agent and application thereof in transportation and fresh keeping of tissue culture seedlings out of culture medium

Abstract

The invention discloses a fresh-keeping agent and application thereof in transportation and fresh keeping of tissue culture seedlings out of a culture medium. The fresh-keeping agent is prepared by taking the culture medium as a substrate and adding white sugar and an activity keeping agent into the substrate and mixing the raw materials, the fresh-keeping agent can be applied to the tissue culture seedlings out of the culture medium, nutrients required by plants and components for keeping plant activity are added and sprayed on seedlings out of the culture medium in a liquid fertilizer manner, so that on one hand, the nutrients required in the transportation process of the tissue culture seedlings are provided, on the other hand, the tissue culture seedlings can keep activity in the transportation process, meanwhile, the conditions of dead leaves, yellow leaves and weak growing power are greatly reduced, and the survival rate of the tissue culture seedlings is increased; and in addition, according to the scheme, the application method of the fresh-keeping agent is simple, operation is convenient, and the fresh-keeping agent can be suitable for industrial implementation.

Description

technical field [0001] The invention relates to the technical field of antistaling agents, in particular to an antistaling agent and its application in transportation and preservation of tissue cultured seedlings from culture medium. Background technique [0002] Plant tissue culture, that is, plant aseptic culture technology, is based on the theory that plant cells are totipotent, using isolated plant organs such as roots, stems, leaves, stem tips, flowers, fruits, etc.) tissues (such as cambium, epidermis, Cortex, medullary cells, endosperm, etc.) or cells (such as megaspores, microspores, somatic cells, etc.) and protoplasts can induce callus under sterile and suitable artificial medium and artificial conditions such as light and temperature Tissues, adventitious buds, adventitious roots, and finally the discipline of forming a complete plant. [0003] When the current tissue culture seedlings are shipped and transported, if the culture medium is carried, there will be a...

AI Technical Summary

Problems solved by technology

[0003] When the current tissue culture seedlings are shipped and transported, if there is a culture medium, there will be a real problem of heavy transportation, increased transportation costs and easy to be infected with bacteria on the way, resulting in pollution and loss

If the medium is removed for transportation, the weight can be greatly reduced, the transportation cost can also be reduced, and the loss of pollution caused by transportation can also be reduced; Then the probability of yellow leaves, dead leaves, and weakening of tissue culture seedlings will increase, which will further affect the survival rate of transplantation. Therefore, it will be a topic of positive and practical significance to conduct research on the fresh-keeping and transportation of tissue culture seedlings.

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