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Pharmaceutical composition and application thereof in preparation of drug for treating bone marrow hematopoietic function disorder

A technology of hematopoietic function and composition, applied in the field of pharmaceutical composition, which can solve the problem of weakening the short-term differentiation ability of cells

Inactive Publication Date: 2014-02-19
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the overexpression of HOXB4 as a transcription factor may affect other biological aspects of cells, and increasing the expression level of HOXB4 can enhance CD34 + The proliferation and self-renewal of cells also weakens the short-term differentiation ability of cells (Zhong Bo, Ye Feng, Wang Tao, etc. Cloning and identification of survivin gene promoter fragments with different activities [J]. Journal of Third Military Medical University, 2011 ,33(11):1115-1118; Bell E.Transplantation:Making space for HSCs.Nature Biotechnology.2008;8(1):4-5)

Method used

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  • Pharmaceutical composition and application thereof in preparation of drug for treating bone marrow hematopoietic function disorder
  • Pharmaceutical composition and application thereof in preparation of drug for treating bone marrow hematopoietic function disorder
  • Pharmaceutical composition and application thereof in preparation of drug for treating bone marrow hematopoietic function disorder

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Embodiment Construction

[0013] The present invention will be described in further detail below in conjunction with embodiment.

[0014] Materials and Methods

[0015] 1 Carriers and reagents

[0016]pAD-SDF-1-IRES-EGFP, pAD-HOXB4-IRES-EGFP, pAD-SDF-1-(GlySer)3-HOXB4-IRES- The EGFP adenovirus vector was constructed by the applicant and can be distributed to the outside world. For the construction method, see "Zhang Pei, Chen Tingting, Ye Feng et al. Effects of mesenchymal stem cells expressing SDF-1 / HOXB4 fusion gene on in vitro expansion and stemness maintenance of hematopoietic stem cells." Journal of Third Military Medical University. 2013.35 (7). 589 -594."

[0017] The medium DMEM+10% fetal bovine serum was purchased from Invitrogen Company, the MACS magnetic separation kit was purchased from Miltenyi Company, the FITC-labeled mouse anti-human CD45 and control IgG2b and IgG1 were purchased from BD Company, and the lymphocyte separation liquid TBD Company.

[0018] 1.2 Sources of experimental ...

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Abstract

The invention relates to a pharmaceutical composition, comprising the medicinal ingredients of mesenchymal stem cells (MSCs) of expressing SDF-1 / HOXB4 fusion gene and CD34<+> hematopoietic stem cells (HSCs). An adenoviral expression vector is built by the invention; the SDF-1, HOXB4 and fuse protein SDF-1 / HOXB4 thereof are respectively expressed; the effects of cotransplantation of the mesenchymal stem cells of expressing the SDF-1 / HOXB4 fusion gene combined with the fetal blood CD34<+> hematopoietic stem cells on radiation-injured mice are observed. The result shows that hematopoietic reconstitution and implantation can be facilitated by cotransplantation of the mesenchymal stem cells (MSCs) of expressing the SDF-1 / HOXB4 fusion gene combined with the fetal blood CD34<+> hematopoietic stem cells (HSCs), and the success rate of transplantation is also improved.

Description

technical field [0001] The invention relates to a pharmaceutical composition, in particular to a medicine for treating bone marrow hematopoietic dysfunction. Background technique [0002] Hematopoietic Stem Cells (HSCs) transplantation is one of the effective methods for the treatment of blood diseases, bone marrow hematopoietic dysfunction caused by radiotherapy and chemotherapy for malignant solid tumors, and various ischemic anemias (Borhane G; Mickie B.Transplantation of human hematopoietic repopulating cells: mechanisms of regeneration and differentiation using human-mouse xenografts. Current Opinion in Organ Transplantation. 2008;13(1):44-52.). However, the low homing rate and limited self-proliferation ability of HSCs after transplantation have become the bottleneck problem restricting the hematopoietic reconstruction of bone marrow. In recent years, with the progress of basic and clinical research on bone marrow mesenchymal stem cells (MSCs), many studies have confi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P7/00A61K35/14
Inventor 张勇张佩陈婷婷范文霞钱峰华裴莉徐双年邹仲敏
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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