Method for artificial fecundation of hybridization frog

Abstract

A method for manually breeding hybrid frogs belongs to the technical field of seafood product breeding and aims to provide the method for manually breeding hybrid frogs with high survive rate, rapid growing speed and strong disease resistance. The method has the key technical point that a sexually mature female frog is selected, a sucker is inserted in the matrix of the frog from the cloacal opening thereof, after mature egg grains are sucked out of the matrix, the egg grains are wedged in a glass service filled with physiologic saline; the other sexually mature male frog is selected, the needle tube of an injector is directly inserted in the spermary of the male frog, and the physiologic saline is injected in the spermary, after a sperm suspension is formed, the sperm suspension is drawn out and wedged in the glass service containing the egg grains, the glass service is bobbled to cause the sperm and the egg cell to combine, thereby generating a germ cell, and then the germ cell is transplanted in a hatching pond, the germ cell disperses through lightly moving the water in the pond, and the growth and the hatch of the embryo are managed according to the conventional method, thus the hybrid frog is obtained. The survive rate of the hybrid frog reaches over 95 percent, the growing time is five to six months, and the avoirdupois of the mature frog can reach about 1200g.

Description

technical field [0001] The invention relates to a method for artificially breeding hybrid frogs, belonging to the technical field of aquaculture. Background technique [0002] Frog is an important economic animal and has many important economic values: frog meat is not only a rare delicacy, but also has high medicinal value; frog skin is a good raw material for grass leather; High-quality lubricating oil for instruments; the head, limbs and internal organs of frogs are high-quality feed. Frogs are good at catching insects and are known as "farmland guards". In the past, people mainly obtained frogs from nature. With the development of society, the increase in the catch of frogs and the deterioration of the growth environment of frogs have resulted in fewer and fewer wild frogs. In order to meet the increasing demands of human beings, for a long time, the source of frogs has mainly relied on artificial breeding. At present, the species of artificially bred frogs are mainly...

AI Technical Summary

Problems solved by technology

However, due to the breeding and mixed breeding of single or multi-species frogs for a long time, their close relatives and blood types are similarly mated and reproduced, resulting in the degradation of their germplasm, weakened disease resistance, and decreased survival rate, which is generally only 30-40%; The growth rate is slow, from ovulation, fertilization, hatching, tadpoles, to commercial frogs, generally takes about one year