Renal mesenchymoma model and its establishing process and application

A method of establishing a method and a technology of an animal model, which is applied in the field of establishment of cell lines, can solve the problems of lack of research reports on animal models of mesoderm nephroma, and achieve the effects of easy production, high tumor formation rate, and wide application prospects

Inactive Publication Date: 2007-08-22
潮州市中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since there is no report on the successful establishment of a mesodermal tumor cell line, there is no research report on an animal model of human mesodermal nephroma

Method used

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  • Renal mesenchymoma model and its establishing process and application
  • Renal mesenchymoma model and its establishing process and application
  • Renal mesenchymoma model and its establishing process and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Establishment of a cell line with fibrosarcoma properties

[0054] 1. Materials and methods

[0055] 1 Department establishment process

[0056] Aborted embryos were obtained from the Obstetrics and Gynecology Department of Chaozhou Central Hospital, 8-13 weeks old, and the tissue was obtained with the consent of the patient or family members. Under aseptic conditions, excise embryonic thigh muscle tissue, soak in saline containing double antibodies (penicillin 100μg / ml, streptomycin 100μg / ml) and amphotericin B (2μg / ml), and use double antibodies and amphotericin Rinse three times each with sterile normal saline and DMEM culture solution of element B, and cut into 0.5-1mm 3 Small tissue pieces, seeded in a 24-well or 12-well culture plate. Attach small tissue pieces to the bottom of the culture plate, add a small amount of culture solution containing DMEM / 10% serum, and place the culture plate at 37°C with 5% CO 2 and a certain humidity of CO 2 Cultivat...

Embodiment 2

[0073] Example 2: Establishment of an animal model of mesodermal nephroma

[0074] MS0504-N cells were inoculated under the renal capsule of nude mice (the specific method was the same as in Example 1, except that the cells were implanted under the renal capsule), and the subcutaneously inoculated nude mice were 1-2 months after tumor inoculation, on the back of the nude mice. The growth of the tumor could be found (Figure 12), and the mass was removed for histopathological examination. All nude mice were subjected to pathological examination of the important organs of the whole body, paying attention to whether there were local lymph nodes and systemic metastasis. The rate of tumor formation under the renal capsule was 100%. The tumor and kidney tissues, as well as liver, lung and heart tissues were removed. From the gross appearance, the tumors are single or multiple, solid, and some tumors have a relatively complete renal capsule, and the tumor can also infiltrate into ext...

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Abstract

The establishing process of mesoblastic nephroma animal model includes the following steps: primary culture of embryo muscle cell of mammal; subculture after the cell grows to fuse; extracorporeal culture for 3-8 months to obtain cloning cell in the culture and eternal tumor cell line; passaging for several times after malignant cell transformation to obtain monoclinic cell strain via monoclonal culture; nude mouse hypodermic transplantation of the monoclinic cell strain; identifying the tumor cell strain by means of immunocytochemistry method or molecular biological method; transplanting the obtained fibrosarcoma cell strain to nude mouse renal diolame to obtain the mesoblastic nephroma animal model. The mesoblastic nephroma animal model provides the research of mesoblastic nephroma pathogenesis and treatment with technological platform.

Description

technical field [0001] The invention relates to a method for establishing a cell line, in particular to an animal model of mesodermal nephroma and its establishment method and application. Background technique: [0002] Tumor cells cultured in vitro are good biological models for studying the biological characteristics of tumor cells, the mechanism of carcinogenesis and cancer treatment. The conventional method for the establishment of tumor cell lines is to use tumor tissue for culture. If immortality is obtained after long-term culture in vitro, it is an immortalized tumor cell line. Another way to establish a tumor cell line is to culture normal cells, and then introduce oncogenes such as telomerase gene [Biochem Biophys Res Commun.2004; 315(3):643-651.], SV40 large T Antigen [Biochim Biophys Acta.2005; 1722(1):6-14.], myc gene [Cancer Res.2005; 65(6):2179-2185.] and human papillomavirus gene [Int JCancer.2004; 110(3):313-319.], the cells can survive for a long time, ob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/073C12N5/09
Inventor 杨立业陈强郑佳坤黄天华刘桂英李文玉张松温建成黄革
Owner 潮州市中心医院
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