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Method for improving success rate of colon cancer PDX model

A colon cancer and success rate technology, applied in the medical field, can solve the problems of instability and low success rate of colon cancer models, and achieve the effect of improving the tumor formation rate

Inactive Publication Date: 2018-10-23
FUDAN UNIV SHANGHAI CANCER CENT
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AI Technical Summary

Problems solved by technology

[0020] The purpose of the present invention is to solve the technical problems existing in the prior art: to provide a method for improving the success rate of colon cancer PDX models, especially a high-infiltration Method for preparing a high-metastasis and stable human colon cancer xenograft tumor mouse model

Method used

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  • Method for improving success rate of colon cancer PDX model
  • Method for improving success rate of colon cancer PDX model
  • Method for improving success rate of colon cancer PDX model

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0039] Example 1 Establishment of Human Colon Cancer Transplanted Tumor Mouse Model

[0040] 1. Specimen collection

[0041] Inclusion criteria: new colon cancer patients with clear pathological diagnosis; complete clinical data; signed informed consent;

[0042] Collect basic clinical data of patients, such as age, gender, TNM stage, etc. Basic data before treatment include tumor marker levels, blood routine, biochemistry, imaging examination results, etc. Treatment data include medication regimens, adverse reactions, etc. Follow-up data include treatment Effect, survival period, etc., the above data will be carried out at the same time during the research process, and a patient data database will be established;

[0043] Collect isolated patient tumor tissue through surgical resection and needle biopsy without other tissues attached; the size of the tissue sample is crucial to the success rate of the established model, and as many tissue samples as possible are obtained if ...

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Abstract

The invention relates to the field of medicines, relates to a method for establishing an animal model, and particularly relates to a method for establishing a human colon cancer transplanted carcinomamouse model. A preclinical tumor model is made through preprocessing mice with prostaglandin E2 and inoculating immunodeficient mice (Balb / c or NOD / SCID) with the in-vitro in-situ tumors of a patientagainst the defects of low establishing success rate and instability of existing colon cancer models. The invention provides a method for improving the success rate of a colon cancer PDX model, and especially provides the method for preparing the high-infiltration and high-metastasis stable human colon cancer transplanted carcinoma mouse model. The model has great values for preclinical evaluation, treatment and prognosis of the colon cancer.

Description

technical field [0001] The invention relates to the medical field, and relates to a method for establishing an animal model, in particular to a method for establishing a human colon cancer xenograft tumor mouse model. Background technique [0002] Colon cancer is a common malignant digestive tract tumor in gastrointestinal surgery, with high morbidity and mortality. In 2010, the incidence of new cases of colorectal cancer in my country ranked fifth among males (23.38%) and third among females (18.30%), and the mortality rates accounted for 11.39% and 8.64% respectively. And rise. In order to discover the specific targets of new drugs and accurately predict the curative effect, the study of tumor animal models is indispensable. [0003] There are three main types of animal tumor models currently used in preclinical research: genetically engineered models (GEMs), cell line-derived xenograft models, and patient-derived xenografts (patient-derived xenografts, PDX). GEMs often ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/13A61K31/5575
CPCA61K31/5575A61K35/13
Inventor 李文涛李晨蔚王广志张蕾周春燕
Owner FUDAN UNIV SHANGHAI CANCER CENT
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