Rat liver cancer model with different liver matrix hardness background and its preparation method

Abstract

The invention relates to a rat liver cancer model with different liver matrix hardness backgrounds and a preparation method of the rat liver cancer model. The method for preparing the rat liver cancer model with different liver matrix backgrounds is characterized by specifically comprising the following steps of: after grouping the rats, injecting pure carbon tetrachloride to an abdominal region in a hypodermic manner; injecting a carbon tetrachloride-olive oil solution to the abdominal region in the hypodermic manner; detecting through a texture analyzer to form the rat model with different liver matrix hardness; taking liver cancer cells which are in-vitro cultured and grown well and injecting the liver cancer cells to the rat to form hypodermic liver cancer cells; in-situ planting the formed liver cancer tumor source to the rat model with different liver matrix hardness to obtain the rat liver cancer model with different liver matrix hardness backgrounds. The rat liver cancer model with different liver matrix hardness backgrounds can be used for well displaying liver cancer malignant pathological characteristics under the liver matrix hardness interference, simulating and reproducing pathological characteristics of clinical liver matrix hardening background liver cancer and solving the problems that an ideal experiment system, an animal model and the like are lacked in reaching the development of the liver cancer due to the liver matrix hardness changes.

Description

Technical field [0001] The invention relates to the technical field of medical evaluation and detection, in particular to a rat liver cancer model with different liver matrix hardness background and a preparation method thereof. Background technique [0002] Ideal experimental animal disease models and their specific pathophysiological characteristics are an important basis for the study of corresponding diseases. They play a huge role in elucidating the molecular pathological mechanism of diseases and evaluating the effects of diagnosis and treatment. However, the complexity of the disease itself determines that experimental animal models are often only It can simulate a certain pathological stage / process of a disease or a certain specific pathological feature, and it is still far from reflecting the actual pathophysiological condition of the clinical disease. Animal disease models based on the physical characteristics of the disease are even rarer. The pathogenesis of liver can...

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Problems solved by technology

In the study of human liver cancer transplantation animal models, immunodeficient animals are often used, including nude mice, nude rats, combined immunodeficiency nude mice and severe combined immunodeficiency (SCID) mice. It has solved the problem of the growth of human liver cancer tissue in animals, but the impact of cellular immunity deficiency on metastasis research, human-mouse mixed cells in the liver cancer microenvironment, and high feeding conditions have always restricted the wide application of this type of model.

However, many induced liver fibrosis / cirrhosis animal models are based on pathological liver fibrosis and cirrhosis, lack of joint modeling with liver cancer, a common downstream disease, and especially the lack of detection of important physical parameters of matrix hardness, making this type of model Only limited to liver fibrosis research, unable to explore the impact of fibrosis on the occurrence and development of liver cancer

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