Human endometrial adenocarcinoma cell line and establishing method thereof

A technology of endometrium and its establishment method, which is applied in the field of human endometrial cancer cell line and its establishment, can solve the problems of large differences in biological properties and low biodiversity of endometrial cancer, and achieve stable properties and successful growth. High tumor rate and good uniformity

Active Publication Date: 2017-03-08
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing human endometrial cancer cell lines have low biodiversity and are quite different from the clinical biological characteristics of endometrial cancer.

Method used

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  • Human endometrial adenocarcinoma cell line and establishing method thereof
  • Human endometrial adenocarcinoma cell line and establishing method thereof
  • Human endometrial adenocarcinoma cell line and establishing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. The establishment of human endometrial cancer cell line hEMC20121018.

[0056] The postoperative tumor tissues of patients with endometrioid adenocarcinoma were clinically collected for primary cell culture of endometrial carcinoma.

[0057] The patient's medical records: Xu Fenglan, aged 62 years. The clinical manifestations are poorly differentiated adenocarcinoma with squamous metaplasia, 1 / 3 of the cervix is ​​infiltrated, and the cervix is ​​infiltrated.

[0058] Immediately after the tumor tissue was isolated, it was kept in an ice box at low temperature, and returned to the laboratory within 30 minutes for cell isolation and primary culture.

[0059] Primary culture: take fresh isolated tissue after surgery and infiltrate it in RPMI-1640 culture medium, send it to the laboratory for processing, wash the tissue specimen with PBS solution 5 times, trim the tissue with tissue scissors, remove necrosis, and then rinse with PBS solution in large quantities After ...

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Abstract

The invention discloses a human endometrial adenocarcinoma cell line and an establishing method thereof. The human endometrial adenocarcinoma cell line is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number CGMCC No. 12290. The establishing method comprises the following steps: (A) acquiring a fresh clinical endometrial adenocarcinoma surgical resection specimen, and performing cell separation primary culture with an II collagenase digestion method; (B) when the cell confluence degree is about 90 percent, performing cell passage culture to finish line establishment of hEMC20121018. The human endometrial adenocarcinoma cell line has a stable cell biological characteristic, an HER-2 gene encoding protein is expressed highly, and a tumor can be formed in a mammal. The human endometrial adenocarcinoma cell line can be directly applied to relevant important researches of cell biology, molecular biology and antitumor drug screening, and is an ideal human endometrial adenocarcinoma cell line applied to fundamental researches and pre-clinical applications.

Description

technical field [0001] The invention relates to the field of biological cells, in particular to a human endometrial cancer cell line and its establishment method. Background technique [0002] Endometrial cancer is one of the common reproductive tract malignancies in women, and endometrial cancer is found in developed regions such as the United States and Europe. At present, it is close to 50% of new gynecological malignant tumors. According to the report of the National Cancer Institute of the United States, there were 54,870 new cases of endometrial cancer in the United States in 2015, and 10,170 deaths. Although the incidence of endometrial cancer in developing countries is lower than that in developed countries, its mortality rate of 34% (21000 / 62000) is significantly higher than that of developed countries (21% (29000 / 136000). The vast majority of endometrial adenocarcinomas are more common in menopausal and postmenopausal women. Since the pathogenesis, invasion and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12R1/91
Inventor 辛彦孟丽荣孙丹
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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