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Method for establishing model of oral cancer and lymphatic metastasis of mice

A technology for transferring models and constructing methods, which is applied in the directions of active ingredients of heterocyclic compounds, animal husbandry, etc., can solve the problems of incompetence of models, and achieve the effect of simple and natural method, ideal effect and high morbidity rate.

Inactive Publication Date: 2012-07-25
GUANGXI MEDICAL UNIVERSITY
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Problems solved by technology

If it is necessary to explore the temporal and spatial continuity of cancer from development to metastasis, the model is not competent

Method used

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Experimental program
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Embodiment Construction

[0017] (1) Animal selection and grouping: 102 6-week-old male Balb / c mice were used as experimental mice, and randomly divided into experimental group and control group;

[0018] The conditions of the experimental mice are: male Balb / c 6 weeks old, clean grade, body weight (23.52±1.15 g), at room temperature 18-25°C, humidity 30%-50%, standard feed, 24h light-dark cycle conditions feeding;

[0019] (2) Reagent preparation: use 4-nitroquinoline-1-oxide (4NQO) (manufactured by Sigma, USA), prepare reagent A at a concentration of 0.1% with sterile distilled water, and store it in a refrigerator at 4°C in the dark. When used, make 200mg / L reagent A with tap water, put it in a light-proof bottle and let the mice drink freely; tap water is reagent B;

[0020] (3) Animal feeding. The mice in the experimental group were fed with reagent A, and the mice in the control group were only fed with reagent B for 20 weeks each; after 20 weeks, the mice in the experimental group were stopped ...

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Abstract

The invention discloses a method for establishing a model of oral cancer and lymphatic metastasis of mice, which is characterized by including mouse selection and grouping, reagent preparation, mouse feeding, experimental observation and conclusion drawing. The method specifically includes: using 200mg / L 4-nitroquinoline-1-oxide to feed Balb / c (Bagg albino / c) mice for 20 weeks by a drinking method; continuously using tap water to feed the mice to the fortieth week and observing to the fortieth week; obtaining the model of oral cancer and lymphatic metastasis of the mice which have development metastasis extremely similar to human oral cancer, namely location diversification, a full metastasis process of normal mucosa, dysplasia, carcinoma in situ, squamous carcinoma and cervical lymph nodes. The method is simple, natural and ideal in effect, guarantees high morbidity, diversified disease locations (lip, palate, gum, tongue and mouth floor) and high submandibular lymph node metastasis rate, and a novel powerful tool for preventing and treating human oral cancer is provided.

Description

technical field [0001] The invention relates to a method for constructing an animal model of oral cancer and lymphatic metastasis, in particular to a method for constructing a model of mouse oral cancer and lymphatic metastasis. Background technique [0002] Oral cancer is a common malignant tumor that occurs in the oral mucosa. It is characterized by squamous cell carcinoma, including cancers that occur in the tongue, buccal, floor of the mouth, lips, and gums. Infiltration and metastasis occur earlier, and Lymphatic metastasis is the main disease. Once metastasis occurs, the survival rate will be significantly reduced. At present, there is no radical cure in clinical practice. Therefore, research on animal models of oral cancer, especially lymphatic metastasis models, has been paid attention to. At present, the establishment of mouse oral cancer models mostly adopts xenograft tumor method, such as orthotopic transplantation of human oral cancer cell lines in nude mice, or...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K31/47
Inventor 于大海李晶黎明武
Owner GUANGXI MEDICAL UNIVERSITY
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