Construction method of nasopharyngeal carcinoma tumor model and application

A construction method and technology of nasopharyngeal carcinoma, applied in the field of nasopharyngeal carcinoma tumor model construction, can solve the difficulty of nasopharyngeal carcinoma tumor model construction and other problems

Pending Publication Date: 2021-03-12
THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on this, it is necessary to provide a new method and application of nasopharyngeal carcinoma tumor model construction in view of the difficulty in constructing traditional nasopharyngeal carcinoma tumor models

Method used

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  • Construction method of nasopharyngeal carcinoma tumor model and application
  • Construction method of nasopharyngeal carcinoma tumor model and application
  • Construction method of nasopharyngeal carcinoma tumor model and application

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Experimental program
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preparation example Construction

[0054] In some embodiments, the method for preparing a nasopharyngeal carcinoma cell line capable of expressing luciferase comprises: infecting C666-1 cells with a lentivirus carrying a luciferase gene.

[0055] In some embodiments, the step of obtaining the nasopharyngeal carcinoma cell line suspension expressing luciferase comprises: adding the cell concentration to (0.8~1.2)×10 6 The luciferase-expressing nasopharyngeal carcinoma cell culture fluid per ml was mixed with Corning Matrigel. Experiments have proved that using Corning Matrigel to prepare nasopharyngeal carcinoma cell line suspension for subcutaneous tumor formation can speed up the growth of subcutaneous tumors and save experimental time.

[0056] In some embodiments, the tissue adhesive can be selected from 3M Vetbond.

[0057] In some embodiments, the step of taking subcutaneously cultured nasopharyngeal carcinoma tumors and cutting them into tumor tissue pieces includes: taking out the nasopharyngeal carcino...

Embodiment

[0065] Embodiment: the method specific steps of described model making are as follows:

[0066] Step S1: Establishing a nasopharyngeal carcinoma cell line with stable high expression of luciferase, specifically:

[0067] Step S1a: C666-1 cells in good growth state were cultured, and the day before virus infection, 2×10 5 C666-1 cells were cultured on 6-well culture plates, and the C666-1 cells in logarithmic growth phase were infected with luciferase (luciferase / Luc) gene lentivirus, added serum-free DMEM culture medium, and placed at 5% CO 2 , Cultivate in a 37°C incubator for 6 hours, then replace with normal medium, and observe the transfection effect after 48 hours;

[0068] Step S1b: Monoclonal screening was performed on the infected cells with a culture medium containing 3 μg / ml puromycin, and C666-1-Luc cells stably and highly expressing luciferase were selected.

[0069] Step S1c: After the purified luciferase-infected human nasopharyngeal carcinoma cell line C666-1...

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PUM

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Abstract

The embodiment of the invention provides a construction method of a nasopharyngeal carcinoma tumor model. The construction method comprises the following steps of inoculating nasopharyngeal carcinomacell line suspension under the skin of a non-human animal, and culturing; after the non-human animal bears the tumor, taking a nasopharyngeal carcinoma tumor cultured subcutaneously, and cutting the nasopharyngeal carcinoma tumor into tumor tissue blocks; inoculating the tumor tissue blocks on the nasopharynx of a normal non-human animal by using an inoculating needle; and adhering an inoculationport and the tumor tissue blocks by using tissue adhesive by using an inoculation needle, and culturing.

Description

technical field [0001] The invention relates to the technical field of disease models, in particular to a construction method and application of a nasopharyngeal carcinoma tumor model. Background technique [0002] Nasopharyngeal carcinoma is one of the most common tumors in my country, and it is also the head and neck tumor with the highest morbidity and mortality in my country. Among them, Guangdong, Guangxi, Fujian, Hunan and other provinces are more common. Nasopharyngeal carcinoma is mostly poorly differentiated squamous cell carcinoma, known for its high invasiveness, easy metastasis, and easy recurrence. The current treatment plan for nasopharyngeal carcinoma has achieved good clinical efficacy, but the treatment of metastatic nasopharyngeal carcinoma is difficult and the prognosis is extremely poor. Difference. Most patients have metastases when they are found, which brings great harm to patients, and further research on it is urgently needed. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/0271A61K49/0008A01K2207/12A01K2227/105A01K2267/0331
Inventor 康敏陈玮王仁生陈思霞周子燕银苑秀申明君覃雅婷
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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