Construction method of human prostatic cancer fresh tissue hormone-independent prostatic cancer nude mouse model

Abstract

The invention discloses a construction method of a human prostatic cancer fresh tissue hormone-independent prostatic cancer nude mouse model. The construction method comprises the steps of subcutaneously hiding a slow-release capsule which contains 10mg of testosterone in a mouse for a week, mixing primary cells which are cultured by human prostatic cancer fresh tissues with an artificial basement membrane, inoculating to the unilateral renal capsule of a BULB/C nude mouse which is replenished with androgen, passaging to the prostate anterior lobe of the mouse to obtain a human hormone-dependent prostatic cancer nude mouse model, carrying out castration until the tumor of the nude mouse grows to be greater than 50mm<3>, passaging the tumor to a nude mouse which is surgically castrated and not replenished with androgen, and after the tumor grows up, carrying out passage to obtain the human prostatic cancer fresh tissue hormone-independent prostatic cancer nude mouse model. In such way, the human prostatic cancer fresh tissue hormone-independent prostatic cancer nude mouse model is closer to the human prostatic cancer; and by using a technology of planting human prostatic cancer fresh tissue via nude mouse variants and a primary cell culture technology as platforms, hormone-dependent and hormone-independent prostatic cancer nude mouse models with the same prostatic cancer tissue source are obtained at the same time.

Description

Technical field [0001] The invention relates to the establishment of an animal model, in particular to a method for constructing a human prostate cancer fresh tissue hormone-independent prostate cancer nude mouse model. Background technique [0002] In the study of the biological mechanism of the occurrence and development of prostate cancer and the evaluation of the effectiveness of treatment measures, various animal models of prostate cancer have become the key tools of the experiment. At present, many scholars at home and abroad have successfully established various animal models of prostate cancer, including the use of cell lines consistent with the biological performance of human primary prostate cancer and fresh tissues of human prostate cancer to construct nude mouse models. The ideal animal model of prostate cancer should be able to simulate the physiological process of the occurrence and development of human prostate cancer. The malignant degree, metastasis habits, histo...

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Problems solved by technology

[0009] The present invention aims to solve the problem in the prior art of using primary cells cultured from fresh human prostate cancer tissue to directly plant in situ and subcutaneously, and provides a new human prostate cancer tissue hormone-independent prostate cancer nude mouse How to build the model

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