Human gastric cancer cell line with 5-fluorouracil resistance and establishing method and application thereof

A gastric cancer cell line and a method for establishing a technology, applied in the biological field, can solve the problems of a large difference in the biological characteristics of gastric cancer and low biodiversity of human gastric cancer cell lines, and achieve high scientific research value and development significance, high homogeneity, and success. The effect of high tumor rate

Active Publication Date: 2015-03-11
SHANGHAI CHEMPARTNER CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a new human gastric cancer cell line with 5-fluorouracil resistance in view of the existing human gastric cancer cell lines with low biodiversity and large differences in biological characteristics from clinical gastric cancer. system and its establishment method and application

Method used

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  • Human gastric cancer cell line with 5-fluorouracil resistance and establishing method and application thereof
  • Human gastric cancer cell line with 5-fluorouracil resistance and establishing method and application thereof
  • Human gastric cancer cell line with 5-fluorouracil resistance and establishing method and application thereof

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Embodiment 1

[0048] The preparation of embodiment 1GAXC066 cell

[0049] Fresh clinical gastric cancer resection specimens were obtained from Nantong Cancer Hospital (patient sex: male, age: 72 years old, ethnicity: Han, native place: Nantong City, Jiangsu Province.), and the clinical diagnosis was poorly differentiated gastric adenocarcinoma.

[0050] Rinse with fresh HBSS buffer (containing 500 U / ml penicillin G, 500 μg / ml streptomycin sulfate and 1.25 μg / ml amphotericin B), cut into small pieces of 20-50 mg, subcutaneously puncture and inoculate in immunodeficient animals SCID mice (SCID mice were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.). 70-90 days after puncture inoculation, the tumor-bearing animals were sacrificed, and the tumor tissues were taken out for primary culture and subculture of cancer cells.

[0051] The primary culture steps are as follows: cut the tumor tissue into small pieces, place them in a culture bottle, and incubate at 37°C ...

Embodiment 2

[0054] Biological characteristics and application of embodiment 2GAXC066 cells

[0055] In the present invention, RPMI1640 is used for culturing and the GAXC066 cells are purified by a differential attachment method, so that they can grow long-term in vitro and pass stably. When the cells are passed to more than 20 generations, the cell properties are gradually stabilized, and the relevant biology, genetics and tissue source identification are carried out until the 50th generation has the same stable properties. Through experimental observation and verification, GAXC066 main cells grown in vitro are in the shape of large willow leaves and have no contact inhibition properties. Genetic studies have confirmed that the cells are aneuploid, with severe chromosome number and structural aberrations, consistent with the genetic characteristics of malignant tumors. The cells can form tumors in nude mice and have tumorigenicity. The corresponding relationship between the cells and th...

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Abstract

The invention discloses a human gastric cancer cell line with 5-fluorouracil resistance and an establishing method and application thereof. The human gastric cancer cell line is preserved in China Center for Type Culture Collection and has a preservation number of CCTCC NO:2013154. The human gastric cancer cell line has the advantages of stable characters, stable multiple passages, high tumor formation rate, short incubation period and good uniformity, and provides a novel experimental material closer to the biological characteristics of clinical tumor for the study of gastric carcinoma. The human gastric cancer cell line has tumorigenicity; the generated tumor has 5-fluorouracil resistance and can be used to analyze the in vitro and in vivo drug sensitivity and resistance correlation, and then establish in vitro and in vivo two related drug screening platforms; and the is cell line is ideal for basic research of gastric cancer and clinical early stage application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a human gastric cancer cell line with 5-fluorouracil drug resistance and its establishment method and application. Background technique [0002] Malignant tumors are currently the main diseases that seriously endanger human health. Among them, gastric cancer is one of the cancers with the highest incidence rate in the world, and it is also the second cause of cancer death in the world, second only to lung cancer. According to the Global Cancer Statistics report in 2011, more than 70% of the new cases and deaths of gastric cancer in the world now occur in developing countries, among which East Asia tops the list, with incidence rates reaching 42.4% for men and 18.3% for women. According to the data listed by the Clinical Oncology Collaborative Center (CSCO) of the Chinese Anti-Cancer Association, there are currently 934,000 new cases of gastric cancer in the world every ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027C12R1/91
Inventor 强福林胡刚汤旭蓁张一心方后顺汪宗宇秦宵然
Owner SHANGHAI CHEMPARTNER CO LTD
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