Application of RAG2 gene knockout rat in establishing personalized tumor treatment model

A gene knockout and RAG2 technology, applied in animal husbandry and other fields, can solve problems such as ethical restrictions, high cost, inconvenient operation, etc., and achieve the effect of high tumor formation rate, short cycle and simple process

Inactive Publication Date: 2015-12-16
INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Due to ethical constraints, high cost, inconvenient operation, etc., experiments using primates close to humans are limited, and clinical observation and research are relatively shallow

Method used

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  • Application of RAG2 gene knockout rat in establishing personalized tumor treatment model
  • Application of RAG2 gene knockout rat in establishing personalized tumor treatment model
  • Application of RAG2 gene knockout rat in establishing personalized tumor treatment model

Examples

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Embodiment 1

[0034] Example 1. Establishing a Personalized Tumor Therapy Model Using RAG2 Gene Knockout Rats

[0035] 1. Obtaining RAG2 knockout rats

[0036] The rat RAG2 gene is located on chromosome 2 of the rat and consists of 3 exons, and the coding sequence is located in exon 3. RAG2 gene knockout rats were created using TALEN technology. The TALEN target sequence designed for the rat RAG2 gene (GeneID: 295953, http: / / www.ncbi.nlm.nih.gov / gene / 295953) is as follows: CTAAAGATTCCTGCTACctcccaccctcttcgttacCCAGCTACTTGCTCGT, where The recognition sequence of the left TALE is as follows: CTAAAGATTCCTGCTAC; the recognition sequence of the right TALE is as follows: ACGAGCAAGTAGCTGG; the middle is a spacer sequence. The sequence of the repeat variable double amino acid residue site (RepeatVariantDi-residue, RVD) of the left TALE recognition sequence is as follows: HDNGNINININNNINGNGHDHDNGNNHDNGNIHD. The RVD sequence for the right TALE recognition sequence is as follows: NIHDNNNINNHDNIININNNN...

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Abstract

The invention discloses application of a RAG2 gene knockout rat in establishing a personalized tumor treatment model. The personalized humanized RAG2 gene knockout rat tumor treatment model is successfully established by transplanting tumor tissues of a patient by using the RAG2 gene knockout rat as a carrier. In the treatment model, tumor models with different abnormal paths are established, so that the condition of the tumor disease of the patient is trustily simulated. Drugs and treatment methods with tumor-resisting functions are evaluated by virtue of characteristics of each patient. The method for establishing the personalized tumor treatment model by virtue of the RAG2 gene knockout rat is simple in step, and the established RAG2 gene knockout rat tumor treatment model has the advantages of being short in period, high in tumor formation rate and low in expense, and can be used for forming tumors for different types of tumor tissues, so that the model can be widely applied to personalized treatment of tumors.

Description

technical field [0001] The invention relates to the application of RAG2 gene knockout rats in establishing individualized tumor treatment models. Background technique [0002] Tumor is one of the most threatening diseases to human beings. Compared with other diseases, the effective rate of clinical treatment of tumor is still low. With the continuous deepening of human genomics, pharmacogenomics and tumor molecular biology research, researchers have a more comprehensive understanding of the characteristics of multiple causes and heterogeneity of tumors. Genome sequencing studies have found that there are few gene mutation duplications among tumors of the same pathological type; the gene mutation spectrum among different individuals of the same type of tumor is very different, which is the biological basis for the different clinical phenotypes of tumors of the same pathological type , It is also the reason why different individuals of the same type of tumor respond very diff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
Inventor 雍伟东王婷婷邱彬邓然
Owner INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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