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Method building mice portable breast cancer tumor cell suspension orthotopic model

A breast cancer tumor and in situ model technology is applied in the field of establishing an in situ model of a mouse transplantable breast cancer tumor cell suspension, which can solve the problems of quantitative and inoculation time, which are difficult to effectively control the tumor formation rate of the tumor model and transplant rejection.

Inactive Publication Date: 2013-10-02
BENGBU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the orthotopic model of transplanted tumor is easy to quantify and the limitation of inoculation time is difficult to effectively control the tumor formation rate and cycle of the tumor model required for the experiment, and the insurmountable transplant rejection among various animal strains is urgently needed. Reproducible orthotopic models of transplanted tumors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Establish a mouse transplantable breast cancer tumor cell suspension orthotopic model in the following steps:

[0023] a) Spontaneous breast cancer tumors in mice were excised under sterile conditions, and washed 3 times with sterile phosphate buffered saline;

[0024] b) Grind the tumor mass in a 400-mesh mesh sieve, place the petri dish for collection under it, rinse the ground cells with sterile phosphate buffer solution, centrifuge twice at 2500r / min for 5min, and the cells sink to the bottom ;

[0025] c) Counting cells, take 10 μL of cells and drop them on the counting plate, and calculate according to the calculation formula (counting up does not count down, counting left does not count right), and the cell concentration is adjusted to 0.7×10 with DMEM culture medium. 6 cell / mL tumor cell suspension;

[0026] d) Select 20 C3H mice in group A, and inject 0.4 mL of tumor cell suspension subcutaneously in the axilla of each mouse's forelimb with a sterile syringe;...

Embodiment 2

[0030] Establish a mouse transplantable breast cancer tumor cell suspension orthotopic model in the following steps:

[0031] a) Spontaneous breast cancer tumors in mice were excised under aseptic conditions, and washed 4 times with sterile phosphate buffered saline;

[0032] b) Grind the tumor mass in a 400-mesh mesh sieve, place the petri dish for collection under it, rinse the ground cells with sterile phosphate buffer solution, centrifuge twice at 2500r / min for 5min, and the cells sink to the bottom ;

[0033] c) Counting cells, take 10 μL of cells and drop them on the counting plate, and calculate according to the calculation formula (counting up does not count down, counting left does not count right), and the cell concentration is adjusted to 0.9×10 with DMEM culture medium. 6 cell / mL tumor cell suspension;

[0034] d) Select 20 C3H mice in group B, and inject 0.3 mL of tumor cell suspension subcutaneously in the axilla of each mouse's forelimb with a sterile syringe;...

Embodiment 3

[0038] Establish a mouse transplantable breast cancer tumor cell suspension orthotopic model in the following steps:

[0039] a) Spontaneous breast cancer tumors in mice were excised under sterile conditions, and washed 3 times with sterile phosphate buffered saline;

[0040] b) Grind the tumor mass in a 300-mesh mesh sieve, place the petri dish for collection under it, wash the ground cells with sterile phosphate buffer solution, centrifuge twice at 2500r / min for 5min, and the cells sink to the bottom ;

[0041] c) Counting cells, take 10 μL of cells and drop them on the counting plate, and calculate according to the calculation formula (counting up does not count down, counting left does not count right), and the cell concentration is adjusted to 1.1×10 with DMEM culture medium. 6 cell / mL tumor cell suspension;

[0042] d) Select 20 C3H mice in group C, and inject 0.3 mL of tumor cell suspension subcutaneously in the axilla of each mouse's forelimb with a sterile syringe; ...

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Abstract

The invention provides a method building a mice portable breast cancer tumor cell suspension orthotopic model. The method building the portable breast cancer tumor cell suspension orthotopic model comprises the following steps that a mice spontaneous breast cancer tumor is grinded in a mesh screen, centrifugation is conducted, tumor cell suspension with cell contraction of (0.7-1.9) * 106 cell / mL is manufactured by nutrient solution in a deployed mode, 0.2 to 0.4 mL of the tumor cell suspension is injected under the front limb armpit skin of each mouse, the mice are bred normally for 9 to 21 days, and the construction of the mice portable breast cancer tumor cell suspension orthotopic model is finished. An injected tumor cell is formed by real C3H mice tumor cells inside the body of the mouse by grinding, the growing process of the breast cancer cell inside the mouse body is simulated, and the mice are bred normally without a particular environment after the injection is finished. After transplantation, lesions are found to be formed by the C3H at the front limb armpit of the mice are found through detection, the method building the mice portable breast cancer tumor cell suspension has the advantages of being high in occurrence rate, short in time, high in tumor formation rate, low in cost and the like, and therefore the method building the mice portable breast cancer tumor cell suspension can be widely applied to experiments of breast cancer treatment.

Description

technical field [0001] The invention relates to a method for establishing an in situ model of cancer cells, in particular to a method for establishing an in situ model of mouse transplantable breast cancer tumor cell suspension. Background technique [0002] Breast cancer is the number one common malignant tumor in women. Since the research on this tumor disease cannot be carried out directly in the body of tumor patients, it can be done with the help of experimental animals, usually through live animal experiments on C3H mice (C3H mice and immune Deficient mice are not immunodeficient). [0003] Experimental animal tumor models are an indispensable tool for anticancer drug experiments. There are three kinds of animal models currently used for tumor research: transplanted tumor orthotopic model, spontaneous tumor model and induced tumor model. It is rarely used in the screening experiments of anticancer drugs. [0004] At present, the orthotopic model of transplanted tumo...

Claims

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Application Information

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IPC IPC(8): A61D7/00
Inventor 蒋志文刘浩蒋琛琛
Owner BENGBU MEDICAL COLLEGE
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