In-vivo tumor engineering human oophoroma tumor model established on basis of collagen aquogel

A collagen hydrogel, ovarian cancer technology, applied in the field of medical tumor models, can solve the problems of lack of stromal cells, limited space, and difficulty in reproducing the tumor microenvironment in vitro, and achieve a simple method, less trauma, and rich sources. Effect

Inactive Publication Date: 2014-01-08
GUANGXI MEDICAL UNIVERSITY
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, in vitro model studies have also proved that the introduction of materials provides a three-dimensional environment for tumor cells, which is closer to the in v

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In-vivo tumor engineering human oophoroma tumor model established on basis of collagen aquogel
  • In-vivo tumor engineering human oophoroma tumor model established on basis of collagen aquogel
  • In-vivo tumor engineering human oophoroma tumor model established on basis of collagen aquogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] 1. Culture of ovarian cancer cells

[0032] Human ovarian cancer cell SKOV3 was purchased from ATCC and cultured in DMEM medium with 10% fetal bovine serum. Cells were grown to more than 80% confluence for passage. Change the medium every two days. Cells were placed at 37°C, 5% CO 2 cultured in an incubator.

[0033] 2. Preparation of injectable collagen hydrogel

[0034] Preparation method of collagen hydrogel: Type collagen is extracted from newborn cowhide by gradient method, dissolved with 0.5M glacial acetic acid, prepared into a 7mg / mL solution and placed at 4°C. When used, the pH value is adjusted to neutral with 1M NaOH. Place it at 37°C for more than 4-5 minutes to complete the liquid-solid phase transition and become a solid hydrogel.

[0035] 3. Construction experiment of tumor engineered ovarian cancer tumor model in vivo

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses application research of an in-vivo tumor engineering human oophoroma tumor model established on the basis of an injectable collagen aquogel composite human oophoroma cells in a noninvasive subcutaneous injection form to a nude mouse. The injectable collagen aquogel stent used as a substrate material is compounded with human oophoroma cell SKOV3 to noninvasively establish the in-vivo tumor engineering human oophoroma tumor model at the nude mouse subcutaneous tissues; and compared with the traditional human oophoroma tumor model established in an independent cell injection mode under the condition of no stent material, the detection on the tumor model indicates that the malignancy of the human oophoroma tumor established on the basis of the injectable collagen aquogel is higher, and is more approximate to that of the human oophoroma tumor, and the in-vivo tumor model established in such a mode has smaller trauma possibility. The tumor model established by the method has higher tumor formation rate (up to 95%), which is about 15% higher than the traditional mode, thereby providing a more reliable tumor model for further research.

Description

technical field [0001] The present invention relates to a medical tumor model—constructing an in vivo tumor engineered human ovarian cancer tumor model based on collagen hydrogel, specifically using injectable collagen hydrogel as a three-dimensional scaffold material and utilizing tumor engineering technology in nude mice Application of constructing human ovarian cancer model. Background technique [0002] The existing tumor model research is mainly based on two types, one is in vitro model, that is, the establishment of a two-dimensional tumor model; the other is the establishment of animal model, mainly a human tumor-bearing nude mouse model. [0003] As far as animal models are concerned, the traditional modeling method is to inject human tumor cells directly into the subcutaneous skin of nude mice. This method is easy to cause the loss of cells, and because there is no matrix support, the connection between cells is not tight, so the tumorigenicity is poor , There is a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/09A01K67/027
Inventor 郑立胡雪峰李力许国杰杨劲松
Owner GUANGXI MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap