Method for establishing microcystin MC-LR promoted diethyl nitrosamine DEN induced rat liver cancer model

Abstract

The invention discloses a method for inducing a rat liver cancer model by injecting diethyl nitrosamine DEN in coordination with microcystin MC-LR into a rat body through intraperitoneal injection. Indexes such as liver weight to body weight ratio, liver appearance, liver histopathological slicing and GST-Pi (Glutathione S-transferase) protein expression level clearly show liver cell injury phase, liver cell hyperplasia-hepatocirrhosis phase and liver cell carcinomatous change process in a rat liver cancer occurring process, and find that the liver cancer model induced by the DEN in coordination with the microcystin MC-LR has a more obvious effect at the same time than that induced by DEN independently; the tumor formation time is shorter; and the model is an ideal model for researching occurrence of the liver cancer of a human body. The model has the characteristic of clearly showing each stage for occurrence of the liver cancer and contributing to research of a liver cancer occurrence process mechanism, detection of precancerous lesion and development of treatment medicaments.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for establishing a rat liver cancer model induced by diethylnitrosamine DEN induced by microcystin MC-LR. Background technique [0002] Liver cancer is one of the most common malignant tumors in my country, known as the "King of Cancer". Therefore, it is necessary to choose a suitable animal model of liver cancer to simulate the pathological process of human liver cancer to help the research of liver cancer. Induced liver cancer model refers to the liver cancer model formed by acting on animals with chemical, physical or biological carcinogens. Among them, diethylnitrosamine (DEN) is a widely used inducer of animal liver cancer, and the process of liver cancer induced by it is very similar to the evolution process of human cancer. At present, DEN has been identified as a carcinogen by the International Cancer Research Center. Microcystin MC is a hepatotoxin, and...

AI Technical Summary

Problems solved by technology

The main problem of this model is that the exposure dose is inaccurate and takes a long time. For example, Li Xiaoyan et al. used diethylnitrosamine (DEN, 80×10 -6 ) Drinking water to establish a rat liver cancer model, the model is the liver cell injury period of 1-8 weeks, the hyperplasia-sclerosing period of 9-16 weeks, and the canceration period of 17-25 weeks

It is suggested that algal toxins have cancer-promoting activity, but the algal toxins used are primary extracts from cyanobacteria, and there is no data on the purity and type identification of algal toxins. There are more than 60 types of algal toxins collected at different times and places The content and type of algal toxins in the water algae samples are different, which increases the uncertainty of the experimental results

In addition, the doses of algae toxins used are 20mg / kg.bw (high dose), 5mg / kg.bw (low dose) (equivalent to the mass of dry algae cells), and no series of concentration experiments have been carried out

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