Method for establishing microcystin MC-LR promoted diethyl nitrosamine DEN induced rat liver cancer model

A diethylnitrosamine, MC-LR technology, applied in the fields of medical science, cyclic peptide components, animal husbandry, etc., can solve the problems of increasing the uncertainty of experimental results, long time, inaccurate exposure dose, etc. The effect of good promotion and application prospects

Inactive Publication Date: 2012-07-04
ANHUI UNIVERSITY
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Problems solved by technology

The main problem of this model is that the exposure dose is inaccurate and takes a long time. For example, Li Xiaoyan et al. used diethylnitrosamine (DEN, 80×10 -6 ) Drinking water to establish a rat liver cancer model, the model is the liver cell injury period of 1-8 weeks, the hyperplasia-sclerosing period of 9-16 weeks, and the canceration period of 17-25 weeks
It is suggested that algal toxins have cancer-promoting activity, but the algal toxins used are primary extracts from cyanobacteria,...

Method used

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  • Method for establishing microcystin MC-LR promoted diethyl nitrosamine DEN induced rat liver cancer model
  • Method for establishing microcystin MC-LR promoted diethyl nitrosamine DEN induced rat liver cancer model
  • Method for establishing microcystin MC-LR promoted diethyl nitrosamine DEN induced rat liver cancer model

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Experimental program
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Embodiment Construction

[0035] 1. Select 15 male SD rats with a body weight of about 150±5g, and give them an adaptation time of 1 week. During the feeding period of the rats, regularly wipe and soak the rat cages with 0.01% benzalkonium bromide solution, and replace the pads in time. material. The feeding temperature is controlled at about 25°C, and sufficient food is given.

[0036] 2. Dilute the DEN solution to a 0.25% DEN solution.

[0037] 3. Dissolve the pure algal toxin powder in methanol, shake for 5 minutes in the dark, and prepare a 1 mg / ml algal toxin mother solution.

[0038] 4. Inject 0.25% DEN solution and cyanotoxin solution into the abdominal cavity of rats, so that the final concentration of DEN is 10 mg / kg, and the final concentration of cyanotoxin is 10 μg / kg.

[0039] 5. The above process is carried out every 5 days for a total of 4 months.

[0040] 6. Always observe the rats' appetite, drinking water, fur, and spirit during the model establishment process.

[0041] 7. Dissect t...

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Abstract

The invention discloses a method for inducing a rat liver cancer model by injecting diethyl nitrosamine DEN in coordination with microcystin MC-LR into a rat body through intraperitoneal injection. Indexes such as liver weight to body weight ratio, liver appearance, liver histopathological slicing and GST-Pi (Glutathione S-transferase) protein expression level clearly show liver cell injury phase, liver cell hyperplasia-hepatocirrhosis phase and liver cell carcinomatous change process in a rat liver cancer occurring process, and find that the liver cancer model induced by the DEN in coordination with the microcystin MC-LR has a more obvious effect at the same time than that induced by DEN independently; the tumor formation time is shorter; and the model is an ideal model for researching occurrence of the liver cancer of a human body. The model has the characteristic of clearly showing each stage for occurrence of the liver cancer and contributing to research of a liver cancer occurrence process mechanism, detection of precancerous lesion and development of treatment medicaments.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for establishing a rat liver cancer model induced by diethylnitrosamine DEN induced by microcystin MC-LR. Background technique [0002] Liver cancer is one of the most common malignant tumors in my country, known as the "King of Cancer". Therefore, it is necessary to choose a suitable animal model of liver cancer to simulate the pathological process of human liver cancer to help the research of liver cancer. Induced liver cancer model refers to the liver cancer model formed by acting on animals with chemical, physical or biological carcinogens. Among them, diethylnitrosamine (DEN) is a widely used inducer of animal liver cancer, and the process of liver cancer induced by it is very similar to the evolution process of human cancer. At present, DEN has been identified as a carcinogen by the International Cancer Research Center. Microcystin MC is a hepatotoxin, and...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K38/12A61D7/00
Inventor 黄蓓徐昌志孙韩艳汪宇沈敬良
Owner ANHUI UNIVERSITY
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