Rubber tree HbAG gene and application thereof

A rubber tree and gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of long sexual generation cycle, limitation of rubber tree breeding efficiency, unsuccessful combination of hybrid combinations, etc. The effect of improving breeding efficiency

Active Publication Date: 2017-07-07
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rubber trees are perennial trees with a long sexual generation cycle. In the breeding of new varieties of rubber trees, there are many problems such as many flowers and few fruits, and low fruit setting

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rubber tree HbAG gene and application thereof
  • Rubber tree HbAG gene and application thereof
  • Rubber tree HbAG gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention. The experimental method that does not indicate specific experimental condition in the following examples, generally according to conventional conditions, molecular cloning (Molecular Cloning: A Laboratory Manual, 3rd ed.) or yeast genetic method experiment guideline (Methodsin Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual , Adams A et al edited, Cold Spring Harbor Laboratory, 1998 publication), or according to the conditions suggested by the manufacturer.

[0020] 1. Cloning of Hevea HbAG gene

[0021] (1) Take the root, stem, stem tip, leaf, xylem, latex, fruit, inflorescence, male flower and female flower of the rubber tree and refer to Invitrogen The steps of the method for extracting RNA by Reagent are carried out to obtain total RNA that meets the requirements of subsequ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a rubber tree HbAG gene, having a nucleotide sequence as shown in SEQ ID NO: 1. The invention further provides a protein coded with the HbAG gene and application of the HbAG gene. The HbAG gene is cloned from a rubber tree for the first time, and the gene belongs to AG subfamily, participates in development of flowers, formation of ovules and maturation of seeds and executes the functions of D genes. Subcellular localization analysis shows that the gene is localized in a cell nucleus and thus conforms to the feature of a transcription factor. The gene is further transferred to arabidopsis to obtain a transgenic plant, and the transgenic plant is discovered to have such phenotypes that the flowering time is obviously advanced, the nutritional growth time is short, and the plant is dwarfed and the like, so the gene plays an important control role in flower organ development and reproductive development of rubber trees. By using the HbAG gene for genetic improvement on rubber trees, an effective gene resource can be provided for shortening the breeding periods of the rubber trees, improving the breeding efficiency and the like.

Description

technical field [0001] The invention relates to the field of biological technology and relates to a rubber tree HbAG gene and its application. Background technique [0002] Hevea brasiliensis, commonly known as rubber tree, is an important economic crop in tropical regions of China. The natural rubber it produces is a strategic resource related to the national economy and people's livelihood. With the increase of domestic natural rubber consumption year by year, due to the limitation of planting conditions and suitable rubber land, it is imminent to increase the output per unit area of ​​rubber trees. Rubber trees are perennial trees with a long sexual generation cycle. In the breeding of new varieties of rubber trees, there are many problems such as many flowers and few fruits, and low fruit setting rate, which leads to the failure of many hybrid combinations, which greatly affects the efficiency of rubber tree breeding. Limitation has become one of the problems to be solv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C12N15/10C12N15/82C12N15/11C07K14/415A01H5/00
CPCC07K14/415C12N15/827
Inventor 位明明王亚杰李维国黄华孙
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap