Porphyra yezoensis mutation breeding method based on pigment mutant selection

Abstract

The invention discloses a porphyra yezoensis mutation breeding method based on pigment mutant selection. The porphyra yezoensis mutation breeding method comprises the steps of: carrying out induced mutation, carrying out enzymolysis and separation on differentiated cells, selecting good regenerated thallus according to an improved variety selection target for carrying out single-strain culture, qualitatively and quantitatively determining the selected good pigment mutant regenerated thallus; carrying out enzymolysis, culturing into regenerated thallus to obtain diploid pure-line protonema through a parthenogenesis technology, and culturing into F1-generation thallus; and crushing the good pure-line protonema and then carrying out a shell seedling culturing test, picking seedlings from shell protonema on a culture web curtain, and carrying out a thallus pigment mutant good strain culturing test in a sea area. According to the invention, the breeding of the large cultivation algae porphyra yezoensis is switched into early-stage prediction and judgment in a laboratory from the later-stage sea cultivation test detection, stable inheritance of the porphyra yezoensis pigment mutant is obtained by using ray radiation treatment, and mutants relevant to the breeding target trait are selected to be used as breeding targets for carrying out breeding.

Description

technical field [0001] The invention relates to a method for mutation breeding of Porphyra zebra. Background technique [0002] Porphyra zebra belongs to large-scale economically cultivated seaweed. Its life cycle includes haploid gametophyte (thallus) stage and diploid sporophyte (filament) stage. Because its fronds are cultivated in sea areas, natural sea areas The selection of breeding materials is difficult, so the research and breeding work needs to take the gametophyte cultured in the laboratory as the research object, through the directional selection of the gametophyte, obtain its diploid spores as germplasm, and observe its traits through sea breeding experiments , based on this, combined with multiple breeding means and methods such as selective breeding and cross breeding to carry out directional genetic improvement work, and cultivate excellent varieties of Porphyra zebra that can be applied to aquaculture production. The traditional laboratory mutation breeding...

AI Technical Summary

Problems solved by technology

The traditional laboratory mutation breeding work mainly focuses on the selection of mutants. For the early transformation stage, the production characteristics of the mutants are mainly used as the main basis for judgment, that is, when the mutant grows to the point where it can be clearly judged whether the economic traits are good or not. At the stage of judging and screening the traits of varieties (lines), it is impossible to identify the algal bodies of excellent strains that have been improved through experiments based on the external mutation traits of the algae (such as the type of chromatophore), and the breeding must be confirmed through marine culture. work effectiveness

Moreover, it takes a long time to directly carry out field breeding in the breeding sea area, and it takes several years to complete the purification of the characters before new varieties can be bred.

The problems of long breeding cycle and low efficiency caused by this technical method need to be solved by new breeding technical means

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