Breeding method for inducing parthenogenesis of corn by propyzamide

A technology of propargin and parthenogenesis, applied in the breeding field of corn inbred lines, can solve the problems of difficult operation, high cost, low doubling rate and the like, and achieve the effects of easy operation, simple method and simple procedure

Inactive Publication Date: 2013-03-13
SHENYANG JINSEGU SPECIAL CORN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The technical problem to be solved in the present invention is: Utilize the inhibitory effect of benzamidrel on cell division, induce corn to directly form seeds through parthenogenety without double fertilization; Carcinog

Method used

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  • Breeding method for inducing parthenogenesis of corn by propyzamide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Preparation of inducer and selection of processing materials

[0027] Choose 50% propargyl acetamide wettable powder on the market, and dilute with water to an inducer with a concentration of 40 μmol / L.

[0028] The processing object is the corn hybrid "Liaodan 565".

[0029] (2) Induction treatment

[0030] Strictly bag the ears before spinning. When most of the filaments on the ears are spit out, cut the filaments short, leaving about 2.5 cm in length, and then spray the filaments with a small sprayer and put them back in the paper bag. Repeated spraying twice a day at 6:00 and 8:00, for 2 consecutive days. Treat 30 spikes.

[0031] (3) Harvest

[0032] Harvest the ears at maturity, carefully peel off the bracts and remove the seeds.

[0033] (4) Identification and reproduction

[0034] In the next season, the seeds will be sown with a single seed, and individual plants that do not meet the breeding goals will be eliminated, bagged and selfed, and 100 ears will be obtained;...

Embodiment 2

[0036] (1) Preparation of inducer and selection of processing materials

[0037] Use the commercially available propargyl propargyl and dilute with water to an inducer with a concentration of 60 μmol / L.

[0038] The processing object is the corn hybrid "Zhengdan 958".

[0039] (2) Induction treatment

[0040] Strictly bag the ears before spinning. When most of the filaments on the ears are spit out, cut the filaments to a length of about 2.0 cm, and then spray the filaments with a small sprayer, and finally put them back into the paper bag. Spraying was repeated 3 times at 6:00, 8:00, and 17:00, respectively, for 2 consecutive days. Process 40 spikes.

[0041] (3) Harvest

[0042] Harvest the ears after maturity, carefully peel off the bracts, remove the seeds, and obtain 180 seeds.

[0043] (4) Identification and reproduction

[0044] In the next season, the seeds will be sown with a single seed, and individual plants with good phenotypes will be selected, bagged and selfed, and 150 ea...

Embodiment 3

[0047] (1) Preparation of inducer and selection of processing materials

[0048] Use the commercially available propargyl dimethochlor and dilute with water to an inducer with a concentration of 80 μmol / L.

[0049] The processing object is "Jundan 20".

[0050] (2) Induction treatment

[0051] It is planted under the condition of space isolation of 1000 meters, and strictly emasculated before dispersing powder. When most of the filaments on the ears are spit out, and the filament length is about 2.0 cm, spray the filaments with a sprayer. Spraying was repeated 3 times at 6:00, 8:00, and 16:00 for 2 consecutive days. Process 50 spikes.

[0052] (3) Harvest

[0053] Harvest the ears after maturity, carefully peel off the bracts, remove the seeds, and harvest 200 seeds.

[0054] (4) Identification and reproduction

[0055] In the next season, the seeds will be sown with a single seed, 150 excellent individual plants will be selected according to the phenotype, bagged and selfed, and 100 ea...

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Abstract

The invention relates to a breeding method for inducing parthenogenesis of corn by propyzamide, belonging to the field of polyploid breeding of plants. The breeding method specifically comprises the following steps of: preparing an inducer with the content of 5 mu mol/L-200mu mol/L by the propyzamide and water; performing induction treatment on filaments, wherein the inducer is sprayed or smeared onto the filaments which grow to about 2.5cm long after emerging, and mature seeds are harvested to get genotype homozygous diploid seeds; and selecting and identifying the seeds according to a conventional method, and selecting a head progeny row which conforms to a breeding goal, thus finally getting a homozygous corn inbred line. The breeding method disclosed by the invention has the positive effects of omitting artificial pollination, being simple, being easy to implement, low in cost, high in efficiency and suitable for large-scale use.

Description

Technical field [0001] The invention belongs to the technical field of plant breeding, especially a breeding method of maize inbred lines. Background technique [0002] In the conventional method of maize inbred line breeding, at least 7 generations are required to complete the selection process of an inbred line, and more generations are required for materials with complex genetic background. In order to shorten the breeding cycle, the existing method at home and abroad is to use the Stock 6 induction line for genetic induction, after inducing the haploid seeds, then use colchicine and other chromosome doubling treatment agents to double them to diploid; Treatment of filaments with chemical reagents such as colchicine induces corn to directly produce homozygous diploid seeds through parthenogenesis. However, these methods have the following disadvantages: [0003] 1. After the Stock 6 genetic induction method is induced, it is necessary to identify each seed and find the induced...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 张喜华
Owner SHENYANG JINSEGU SPECIAL CORN
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